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Uji aktivitas antibakteri ekstrak etanolik umbi mentimun papasan (Coccinia grandis L.Voigt) terhadap Shigella dysenteriae dan Staphylococcus aureus Rukmana, Rizal Maarif; Nugroho, Rahmat Budi; Wisnumurti, Dwi Admani; Wibawa, Andang Arif
Riset Informasi Kesehatan Vol 8 No 2 (2019): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.24 KB) | DOI: 10.30644/rik.v8i2.233

Abstract

Abstrak Latar Belakang: Penyakit infeksi merupakan salah satu penyakit yang menjadi masalah serius di negara berkembang khususnya di Indonesia. Bakteri yang sering menimbulkan penyakit infeksi adalah bakteri Shigella dysentriae dan Staphylococcus aureus. Metode: Serbuk umbi Mentimun Papasan (Coccinia grandis L.Voight) dilakukan ekstraksi dengan metode maserasi. Pelarut yang dipakai adalah etanol 96%. Identifikasi golongan senyawa dilakukan dengan menggunakan pereaksi kimia. Uji aktivitas antibakteri dilakukan dengan metode difusi sumuran. Hasil: Hasil identifikasi golongan senyawa menunjukkan adanya golongan senyawa flavonoid, tanin, saponin, alkaloid dan polifenol. Hasil uji antibakteri ekstrak umbi Mentimun Papasan kosentrasi 1 gr/ml, 2 gr/ml, 3 gr/ml dan 4 gr/ml terhadap bakteri Shigella dysentriae berturut-turut: 18mm, 19,66 mm, 21,23 mm dan 23,33 mm. Hasil uji antibakteri ekstrak umbi Mentimun Papasan kosentrasi 1 gr/ml, 2 gr/ml, 3 gr/ml dan 4 gr/ml terhadap bakteri Staphylococcus aureus berturut-turut: 18,67 mm, 20,67 mm, 23,33 mm dan 27 mm. Kesimpulan: Ekstrak etanolik umbi Mentimun Papasan dapat menghambat bakteri Shigella dysentriae dan Staphylococcus aureus.
Aktivitas sitotoksik dan antiangiogenesis umbi mentimun papasan (Coccinia grandis L.Voight) terhadap sel kanker hela yang diinduksi protein bFGF Djamal, Julia Megawati; P, Jason Merari; Rukmana, Rizal Maarif
Riset Informasi Kesehatan Vol 9 No 1 (2020): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (389.015 KB) | DOI: 10.30644/rik.v9i1.309

Abstract

Background: Cancer is a leading cause of death in Indonesia and in the world. Cervical cancer is one of the most common cancers in women. One of the plants that has the potential to be developed as an alternative treatment for cancer is the papasan cucumber tuber (Coccinia grandis (L) Voight). This study aims to determine the activity of cytotoxic effects on Hela cancer cells and antiangiogenesis activity against Chorioallantoic membrane. Method: In this study, papasan cucumber tubers were extracted with 96% ethanol solvent. The ethanol extract was then continued with fractionation using the liquid-liquid partition method, followed by thin-layer chromatography used to determine of compounds contained in the papasan cucumber tubers. Cytotoxic activity tests were carried out using the MTT assay method and antiangiogenesis tests were carried out using CAM methods of chicken embryos induced with bFGF protein. Results: The results showed that the extract, n-hexane fraction, ethyl acetate fraction and water fraction had IC50 values 986,604 µg/ml, 208,776 µg/ml, 187,824 µg/ml and 553,393 µg/ml. The IC50 value of the n-hexane fraction was then continued with antiangiogenesis testing. The KLT analysis showed that the n-hexane fraction contained alkaloids, flavonoids, tannins, saponins, and terpenes. Antiangiogenesis test result show that the inhibitory at concentration of 104 µg / ml was 17,56%, at a concentration of 208 µg / ml 43,23% and at a concentration of 418 µg / ml 56,78%. Conclusion: n-hexane fraction and ethyl acetate fraction have moderate cytotoxic activity against Hela cancer cells and n-hexane fraction has antiangiogenesis activity. Key words: Coccnia grandis L. Voight, Cytotoxic, Antiangiogenesis, Hela, Chorioallantoic Membrane
UJI AKTIVITAS ANTI BAKTERI EKSTRAK ETANOLIK UMBI AKAR BATU (Coccinia grandis L.Voight) TERHADAP BAKTERI SALMONELLA SP Rukmana, Rizal Maarif; Nugroho, Rahmat Budi; Wisnumurti, Dwi Admani
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 2: September 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i2.8390

Abstract

AbstractSalmonella sp are bacteria that often infect human and animal. This type of bacteria can cause Salmonellosis in human. Previous research has been carried out on secondary metabolites of plants as medicinal ingredients. One of the plants that has potential as a medicine is stone Akar Batu (Coccinia grandis L Voight). This study aims at examining the antibacterial activities of ethanolic extract of stone Akar Batu on Salmonella sp. The extraction method was carried out by maceration and the solvent used was ethanol 96%. Antibacterial activity was conducted using diffusion method. Identification of compound groups of extracts was done by reacting extracts with various chemical reagents. The results showed that stone of Akar Batu powder had a moisture content of 5.99% and a yield of 1.76%. The identification results of ethanolic extract of stone Akar Batu chemical group indicated that the extract contained flavonoids, alkaloids, saponins and tannins. The results of antibacterial activity test have revealed that ethanolic extract of stone Akar Batu can inhibit Salmonella sp.