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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia Jurnal Natur Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Prosiding Seminar Biologi JSDH BERITA BIOLOGI Jurnal Penelitian Sains Jurnal Penelitian Pertanian Tanaman Pangan Menara Perkebunan Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik)

LISDAR I. SUDIRMAN

Bagian Mikologi, Departemen Biologi, Institut Pertanian Bogor

Author-ID : 624776

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Air Quality Bioindicator Using the Population of Epiphytic Macrolichens in Bogor City, West Java . RINDITA; LISDAR IDWAN SUDIRMAN; YONNY KOESMARYONO
HAYATI Journal of Biosciences Vol. 22 No. 2 (2015): April 2015
Publisher : Bogor Agricultural University, Indonesia

Studies about lichens and pollution in South-East Asia are restricted because the lichens are poorly known. A research project about air quality bioindicator using epiphytic macrolichens in Bogor City was conducted from March 2012 until July 2013. Purposive sampling method was applied in 3 plots: plot 1 was in the centre of Bogor Botanical Garden (BBG) that far from busy roads, plot 2 was in a part of BBG adjacent to main and busy roads, and plot 3 was along busy roads and near a factory. In each plot, macrolichens were observed in 8 canary trees using 2 mini quadrats (32 x 20 cm2). The population conditions of epiphytic macrolichens were analyzed and to be used as bioindicator of air quality. Seven genera of macrolichens were found: Coccocarpia, Leptogium, Canoparmelia, Parmotrema, Dirinaria, Physcia, and Pyxine. Plot 1 was having Coccocarpia and Leptogium that were not found in other plots and therefore they can be used as sensitive bioindicators, none of Canoparmelia and Pyxine, a few and infrequent Dirinaria but with larger average coverage (AC = 6.15 cm2), and Physcia was found abundantly (sensitive bioindicator). Conversely, plot 2 and 3 were having none of Coccocarpia and Leptogium, few or many Canoparmelia and Pyxine and therefore appeared to be tolerant, many and frequent Dirinaria but with smaller AC (plot 2 = 2.85 cm2, plot 3 = 1.16 cm2), and few or none of Physcia was found. Being found in all plots, Dirinaria showed a clear pattern of increasing thallus number and decreasing AC from plot 1 to plot 3, so it can be used as tolerant bioindicator. Conversely, Parmotrema had unclear pattern of population condition. Similar researches on different trees are necessary to explore more details regarding epiphytic macrolichens population condition.

Production of Fruiting Body and Antioxidant Activity of Wild Pleurotus Henny Sulistiany; Lisdar Idwan Sudirman; Okky Setyawati Dharmaputra
HAYATI Journal of Biosciences Vol. 23 No. 4 (2016): October 2016
Publisher : Bogor Agricultural University, Indonesia

The HS isolate of wild Pleurotus is a wood-rotting fungus found in Bogor, Indonesia. This study was conducted to determine the growth and fruiting body production of HS isolate on three types of substrates, antioxidant activities, and total phenolic contents (TPCs). HS isolate was grown on Paraserianthes falcataria sawdust (PFS substrates), oil palm empty fruit bunch (EFB) substrates, and mixture of PFS and EFB substrates (M substrates) with proportion 1:1, respectively. Analysis of antioxidant activity of mycelial and fruiting body extracts was conducted using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, whereas TPCs were conducted using Folin–Ciocalteu method. The results showed that HS isolate could grow and produce fruiting bodies on all substrates, but based on all observation parameters, M substrates were the best ones for the growth and fruiting body production of HS isolate with biological efficiency of 88.86%. Fruiting body extract of HS isolate had a better ability to reduce DPPH free radical (IC50, 0.45 ± 0.04 mg/mL) with total phenolic compound of fruiting body extract being higher (4.62 ± 0.08 mg gallic acid equivalent/g extract) than those of mycelia extract. Based on this study, HS isolate is potential as a source of natural antioxidants.

Antioxidant Activity of Endophytic Bacteria Derived from Hoya multiflora Blume Plant and Their Cellular Activities on Schizosaccharomyces pombe Nabila Tsoerayya Gustia Pudjas; Nisa Rachmania Mubarik; Rika Indri Astuti; Lisdar I Sudirman
HAYATI Journal of Biosciences Vol. 29 No. 2 (2022): March 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.2.214-221

Endophytic bacteria isolated from plant tissues can produce the same secondary metabolites as their host plants. One of the metabolites that the bacteria can produce is antioxidants. This research aimed to analyze and measure the antioxidant activities of two endophytic bacteria, i.e. Bacillus siamensis HMB1 and Bacillus aryabhattai HMD4 cultures, derived from Hoya multiflora Blume plant, a tropical epiphytic plant species that grows in Indonesia, and to identify their cellular effects on Schizosaccharomyces pombe. The active compounds went through extraction process, and the antioxidant activities were measured, the extracts went under phytochemical analysis, and their phenol and flavonoid contents were measured. In vitro analysis was carried out using S. pombe. The results of this research indicated that both cultures had antioxidant activities, where HMB1 showed the highest IC50 value (51.18 mg/ml) among all. In vitro analysis indicated that HMD4 bacterial crude extract in 250 ppm concentration showed the highest resistance effect and significantly enhanced S. pombe growth. In addition, the results of the LC-MS analysis suggested that a total of 14 compounds potentially had antioxidant activity.

Pengembangan Teknologi Proses Produksi Bionanokomposit Filler Biomassa Rotan Siti Nikmatin; Lisdar Idwan Sudirman; Mersi Kurniati
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 3 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1281.081 KB)

Rattan biomass is a fiber waste from processing industry of rattan. Its abundant availability, as well as does not threaten the balance of food and feed, make it a potential source as raw material for composite filler of cellulose nanoparticles. To obtain a high cellulose content, it was inoculated with White rote fungi and Aspergillus niger. The experiments were conducted at inoculation time of 15, 21, and 30 days. The results showed that biomass of rattan extracted with White rote fungi and Aspergillus niger reached maximum cellulose content at the inoculation time of 21 days ie 76.47% cellulose, lignin 2.39%, and 20% moisture content. Cellulose has a monoclinic crystal structure, a =7.87; b=10.31; c=10.13 α= γ = 90, β=120. Nanoparticles were produced by disk mill-hummer mill method with variation milling time of 15, 30, and 45 minutes. Collision, friction, and heat for 30 minutes of milling could produce energy that was transferred to the particles and caused cavitation which resulted particles of 16.22-51.30 nm particle size. Production of test piece and prototype of nanocomposite using TSE and injection molding produced material which has 2 phases of crystal structure, namely monoclinic, and orthorhombic phases. The mechanical properties of impact strength was 67.769 J/m and hardness of 79.97 HRR. Thermal properties and density of bionanokomposit showed comparable values with synthetic composites.

Seleksi dan Karakterisasi Bakteri Penghasil Kitinase Penghambat Pertumbuhan Cendawan Patogen Tanaman Azizah Nurmalinda; Nisa Rachmania Mubarik; Lisdar Sudirman
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 1 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Colletotrichum capsici and Fusarium oxysporum are pathogenic fungi that cause anthracnose disease on chili plants (Capsicum annum L.) which can decrease the productivity of chili. The control of the fungus attack is carried out by biocontrol agent of the fungi utilizing chitinase-producing bacteria. Chitinase is an enzyme capable of destroying the fungal cell wall composed of chitin compounds. The aims of the research were to select and to characterize chitinase producing bacteria as biological control agent of Colletotrichum capsici and Fusarium oxysporum. Several methods were carried out in this research, among others: isolation of chitinase bacteria, characterization of chitinase bacteria, measurement of enzyme activity, bacterial antagonistic with pathogenic fungi, observation of fungal hypha damage, and identification of 16S rRNA. The BAE36 and BAD211 isolates are chitinase bacteria isolated from chili plant rhizosphere. The isolate belongs to the Gram-negative bacteria. The inhibitory properties of BAE36 and BAD211 against F. oxysporum and C. capsici are on a scale of 3 (51-75%). The percentage of inhibitory of BAE36 isolate was 66.1% capable of inhibiting the growth of C. capsici and 55% capable of inhibiting the growth of F. oxysporum. The hyphae of C. capsici fungus on the antagonistic test showed a thick and the surface of the hyphae was wavy while the F. oxysporum hyphae was thin and looks empty. Identification of isolate with 16S rRNA described that 99% of BAE36 and BAD211 isolates were similar to Lysobacter enzymogenes. Precipitation of chitinase enzyme of Lysobacter enzymogenes with 30% ammonium sulphate could increase the enzyme activity. The enzyme precipitation could inhibit the growth of mycelia fungi C. capsici and F. oxysporum. Keywords: chili, chitinolytic, Gram-negative, Lysobacter

Mikobiota pada Buah Cabai untuk Pengendalian Hayati Colletotrichum capsici Okky Setyawati Dharmaputra; Lisdar Idwan Sudirman; Melly Fitriani
Jurnal Fitopatologi Indonesia Vol 11 No 5 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Colletotrichum capsici is a pathogenic fungus causing anthracnose on various tropical fruits, especially chilli. Biological control agents have been used as an alternative method to control postharvest diseases. This study aims to examine the antagonistic potential of mycobiota on red chilli fruit against C. capsici. The pathogen was obtained from diseased red chilli fruits collected from three traditional markets in Municipality of Bogor, isolated on potato dextrose agar (PDA) medium containing chloramphenicol (100 mg L-1). Candidates of antagonistic mycobiota were isolated from healthy chilli fruits using serial diution method, followed by pour-plate method on PDA medium containing chloramphenicol (100 mg L-1). C. capsici isolate BIO51046 showed highest pathogenicity on chilli fruit var. IPB Perbani compared to other isolates. Seven isolates of filamentous fungi and 7 yeast isolates were obtained from healthy chilli fruits. Test of antagonism using dual culture method obtained 3 filamentous fungal isolates (Plectosphaerella cucumerina, MF2 and Aspergillus flavus) and 1 yeast isolate (Issatchenkia orientalis) which inhibited the growth of C. capsici BIO 51046 more than 70%. Plectosphaerella cucumerina and I.orientalis did not cause any diseases on chilli fruits var. IPB Perbani. Therefore, these 2 isolates were considered as potential antagonist against C. capsici BIO51046 as the causal agent of anthracnose of chilli

Penggunaan Bakteri Kitinolitik sebagai Pengendali Hayati Colletotrichum capsici pada Tanaman Cabai Dian Syahfitri; Nisa Rachmania Mubarik; Lisdar A Manaf
Jurnal Fitopatologi Indonesia Vol 14 No 4 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Use of Chitinolytic Bacteria as Biological Control of Colletotrichum capsici on Chili PlantsColletotrichum capsici is known as the causal agent of anthracnose disease in chili plant and may cause reduction of crop yield. Chitinolytic bacteria, namely Serratia marcescens KAHN 15.12, Bacillus thuringiensis SAHA 12.12, and BAE 36 were reported to have antagonistic activity against C. capsici. Therefore, a study was conducted to determine the potential of chitinolytic bacteria on controlling C. capsici on chili plants in greenhouse experiment. Three bacterial isolates used as biocontrol agent was formulated by using talcum as carrier materials. The methodologies consisted of characterization of bacterial isolates, formulation of biocontrol agent, viability test of bacterial isolate, efficacy of biocontrol agents in the laboratory and in the greenhouse. Disease severity in the laboratory reached 64% when chili treated with isolate formulation of BAE 36. In the greenhouse, BAE 36 isolate formulation and consortium formulation were able to suppress infection of C. capsici; each was indicated by disease incidence of 25% and 50%, respectively. These results indicated that chitinolytic bacterial formulations could be potencial as biocontrol agents of C. capsici.

Mikobiota pada Buah Pisang Kultivar Lampung untuk Pengendalian Hayati Fusarium semitectum Okky Setyawati Dharmaputra; Lisdar Idwan Sudirman; Evarini Anna Ratnaningsih
Jurnal Fitopatologi Indonesia Vol. 14 No. 1 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Fusarium semitectum is a pathogenic fungus causing fruit rot of banana fruits. Biological control agents have been used as an alternative to control postharvest diseases. The objective of this study was to test antagonistic potential of mycobiota of banana fruits (Musa cuminata cultivar Lampung) against F. semitectum BIO 91055. The tested fungi were isolated from healthy banana cultivar Lampung collected from Gembrong market located in Bogor using serial dilution method, followed by pour plate method. Test of antagonism activity was carried out using dual culture method. Seventeen fungal isolates were isolated, they consisted of 14 filamentous fungal isolates and 3 yeast isolates. Four filamentous fungal isolates inhibited the growth of F. semitectum BIO 91055 more than 70%, they were Aspergillus niger, Cercosporella sp., Plectosphaerella sp., and Trichoderma hamatum. Three isolates (Cercosporella sp., Plectosphaerella sp., and Trichoderma hamatum) did not cause any diseases of banana fruits and they were considered as potential biocontrol agents.

Potensi Khamir sebagai Agens Pengendalian Hayati Colletotrichum capsici, Cendawan Penyebab Antraknosa pada Buah Cabai Okky Setyawati Dharmaputra; Lisdar I. Sudirman; Maria M. Misnawati
Jurnal Hortikultura Indonesia Vol. 7 No. 2 (2016): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

ABSTRACTAntrachnose on chilli fruit caused by Colletotrichum capsici can reduce yield and quality of chilli fruit. Biological control agent can be used as an alternative to control C. capsici. Yeast is one of biological control agent which is potential to control the pathogen. This study was aimed at testing antagonistic potential of yeast on fruits and vegetables against C. capsici. Twenty two yeast isolates were isolated from banana, rambutan, red chilli, tomato, and eggplant fruits. Screening for antagonistic yeast using well test showed that 5 isolates of yeast (CMM-1, CMM-3, CMM-4, TMM-1, and EMM-11) completely inhibited the growth of C. capsici. Based on the result of biocontrol assay of the pathogen in vivo, four yeast isolates (CMM-3, CMM-4, TMM-1, and EMM-11) completely inhibited C. capsici in vivo. Identification using morphological and molecular characteristics showed that these four yeast isolates were Issatchenkia orientalis.Keywords: antagonistic yeast, antrachnose, biocontrol, Issatchenkia orientalis ABSTRAK Antraknosa pada buah cabai yang disebabkan oleh Colletotrichum capsici dapat menyebabkan penurunan produksi dan kualitas buah cabai. Penggunaan agens pengendalian hayati dapat menjadi salah satu alternatif untuk mengendalikan C. capsici. Khamir merupakan salah satu agens pengendalian hayati yang berpotensi mengendalikan C. capsici. Penelitian ini bertujuan menguji potensi antagonistik khamir pada buah-buahan dan sayuran terhadap C. capsici. Sebanyak 22 isolat khamir diisolasi dari buah rambutan, pisang, cabai merah besar, tomat, dan terung ungu. Seleksi khamir antagonis menggunakan uji sumur diperoleh sebanyak 5 isolat khamir, yaitu isolat CMM-1, CMM-3, CMM-4, TMM-1, dan EMM-11 menghambat total pertumbuhan C. capsici. Isolat CMM-3, CMM-4, TMM-1, dan EMM-11 menghambat total pertumbuhan C. capsici in vivo. Berdasarkan hasil identifikasi secara morfologi dan molekuler, isolat CMM-3, CMM-4, TMM-1, dan EMM-11 adalah Issatchenkia orientalis.Kata kunci: antraknosa, Issatchenkia orientalis, khamir antagonis, pengendalian hayati

Fisiologi Lentinus spp. dengan Reaksi Oksidasi pada Medium Agar Asamgalat, Agar Asamtanat, dan Agar Tirosin Yunilda Rosa; Lisdar A.Manaf I. Sudirman; Fahrizal Hazra
Jurnal Penelitian Sains Vol 16, No 1 (2013)
Publisher : Faculty of Mathtmatics and Natural Sciences

Hutan tropis Indonesia kaya akan jenis jamur (Mushroom). Keragaman ini merupakan faktor pendo-rong perlunya dilakukan usaha mengidentifikasi dari jamur-jamur yang ada, salah satunya Lentinus spp. Ada enam Lentinus yang telah diteliti dan dipublikasikan tetapi hanya satu yang berasal dari daerah tropis (Kame-run), yaitu Lentinus squarrosulus. Lentinus berpotensi sebagai makanan atau pencampur makanan. Selain itu juga berpotensi sebagai obat-obatan, diantaranya berkhasiat sebagai antibakteri dan antifungi disamping kha-siat lainnya. Penelitian ini bertujuan untuk mengetahui sifat-sifat fisiologi jamur Lentinus spp. berdasarkan reaksi oksidasi pada medium Agar Asamgalat, Agar Asamtanat dan Agar Tirosin. Data fisiologi ini dipakai se-bagai acuan untuk penelitian selanjutnya dalam produksi senyawa antimikroba. Tahap pelaksanaan meliputi pembuatan stok kultur dan peremajaan biakan murni, melihat reaksi oksidasi pada medium asamgalat agar (AAG), agar asamtanat (AAT), dan agar Tirosin (AT). Hasil penelitian memperlihatkan semua isolat Lentinus spp. menunjukkan reaksi yang positif pada medium AAG, AAT, sebaliknya menunjukkan reaksi negatif pada medium AT. Hal ini ditandai dengan terbentuknya zona coklat terang sampai coklat gelap pada medium AAG dan AAT di sekitar hamparan koloni. Sedangkan pada medium AT tidak ditemui adanya zona hitam di sekitar hamparan koloni.

Co-Authors . RINDITA A.A. Ketut Agung Cahyawan W AUNU RAUF Azizah Nurmalinda Dian Syahfitri Didiek Hadjar Goenadi Evarini Anna Ratnaningsih Fahrizal Hazra Fitratul Aini Fitratul Aini, Fitratul HENNY SULISTANY Henny Sulistiany HENNY SULISTIANY, HENNY Kartosuwondo, Utomo Laksmita Prima Santi Maria M. Misnawati Melly Fitriani Mersi Kurniati Munara, Yayi Nabila Tsoerayya Gustia Pudjas NISA RACHMANIA MUBARIK NURLIANA NURLIANA Okky Setyawati Dharmaputra Okky Setyawati Dharmaputra Okky Setyawati Dharmaputra Okky Setyawati Dharmaputra Prayogo, Yusmani Rika Indri Astuti Ruly Anwar Samingan Samingan Santoso, Teguh SEMPURNA GINTING SEMPURNA GINTING Siti Azzira Rahma Siti Nikmatin Soemartono Sosromarsono Trizelia . Yonny Koesmaryono Yunilda Rosa YUNIMAR YUNIMAR Yusmani Prayogo

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