Indri Lakhsmi Putri, Indri Lakhsmi
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Time Recommendation and Alternative Parameters for Severe Maxillofacial Trauma Reconstruction Indri Lakhsmi Putri1, Magda Rosalina Hutagalung1, Ida Bagus Narmada2, David Sontani Perdanakusuma1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.3160

Abstract

Background: Severe maxillofacial trauma often associated with other injuries, therefore the delay of reconstruction often occur until the patients are stable. Early reconstruction results a better facial function and appearances. Aim: The aim of this study to give the recommendation and alternative parameters for severe maxillofacial trauma reconstruction. Methods: The method of this study is adult patients with bimaxillary and bilateral maxillofacial trauma were eligible for this study, while pregnant women were excluded. Nine patients with maxillofacial trauma were involved in this study and we measured 15 facial anthropometric and 41 lateral cephalometricon 7, 14, 21 days compared to 3 months post-reconstruction. Results: The result of this study showed reconstruction can be planned 14 days after trauma for lower jaw fracture and 21 days after bimaxillary fracture. Lateral cephalometry was a reliable method to measure facial edema following surgery which combined facial anthropometry with lateral cephalometry using parameters that are not affected by edema. This can be applied as an additional guiding tool in surgical planning for maxillofacial trauma patients especially those with bimaxillary and bilateral fractures. Conclusion: Combination of anthropometric and cephalometric parameters which are not affected by edema can be applied as an additional guiding tool in surgical planning for maxillofacial trauma patients.
THE EFFECT OF CONDITIONED-MEDIUM HUMAN ADIPOSE-DERIVED MESENCHYMAL STEM CELL IN APOPTOSIS OF BLADDER CANCER CELLS Mawdudi, Ari Alauddin; Hidayatullah, Furqan; Bachtiar, Indra; Rachman, Arif; Putri, Indri Lakhsmi; Castiglione, Fabio; Djatisoesanto, Wahjoe; Hakim, Lukman
Indonesian Journal of Urology Vol 28 No 1 (2021)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v28i1.653

Abstract

Objective: To determine the effect of conditioned medium human Adipose-Derived Mesenchymal Stem Cells (CM-hADMSC) on apoptosis of urothelial bladder cancer cells. Material & Methods: Bladder (5637) cancer cell lines cultured in conditioned media harvested from human adipose-derived mesenchymal stem cells (hADMSC). Flow cytometry tests were carried out using the Flowcytometry Acquisition cell sorting (FACS) Calibur to measure apoptosis. Results: There was a significant difference in the percentage of late apoptosis in the group receiving culture medium treatment: CM-hADMSC 1: 1 to the entire study group. Further analysis revealed no difference in the average percentage of late apoptosis in groups exposed to culture medium: CM-hADMSC 1: 2 and culture medium: CM-hADMSC 1: 4 (p> 0.05). Conclusion: CM-hADMSC at a 1: 1 dose concentration to culture medium obtain a significant increase of apoptosis in bladder cancer cells.
THE EFFECT OF CONDITIONED MEDIUM ADIPOSE DERIVED MESENCHYMAL STEM CELLS IN UROTHELIAL CARCINOMA CULTURE CELLS VIABILITY Prasetyo, Suryo; Hidayatullah, Furqan; Bachtiar, Indra; Rachman, Arif; Putri, Indri Lakhsmi; Castiglione, Fabio; Soebadi, Doddy M.; Hakim, Lukman
Indonesian Journal of Urology Vol 28 No 1 (2021)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v28i1.654

Abstract

Objective: This study aimed to gives a perspective in CM-ADMSCs effect in urothelial bladder cancer viability. Material & Methods: Human bladder cell carcinoma type 5637 was used as the subject of this in vitro study. This study contains four different groups: untreated control group, Culture medium: hADMSCs with 1:1, 1:2, and 1:4 concentration group. Each group consists of 6 replications to prevent bias of the study. Viability was determined with MTT assay methods and evaluation performed after 48 h exposure of conditioned medium. Results: A post hoc test was conducted to analyze the data. The 5637 bladder cancer cell line demonstrated significantly decreased viability after exposure to culture medium: CM-hADMSCs 1:1 (p: 0.002) compared to the negative control group, but there are no significant differences in viability between the control groups with groups that were exposed to culture medium: CM-hADMSCs 1:2 and culture medium: CM-hADMSCs 1:4 with p: 0.480 and p: 0.060 respectively. Conclusion: Decreased viability of urothelial bladder cancer cells after exposure to CM-hADMSCs occurs at a concentration of 1:1 and Dosage addition more than 1:1 concentration doesn’t give any advantages.