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Perbedaan Kadar TNF-Î± Saliva dan Derajat Kesehatan pada Ibu Hamil Preeklamsia dan Ibu Hamil Tidak Preeklamsia K, Trisnawaty; Salim, Eddy Mart; Yakub, Kemas
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 29, No 3 (2017): Desember
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Pendahuluan: Beberapa teori menyebutkan penyakit periodontal dapat meningkatkan faktor resiko preeklamsia. Gingivitis dapat menyebabkan peningkatan kadar TNF-Î± saliva dan dapat mengakibatkan inflamasi sistemik yang memicu terjadinya preeklamsia. Tujuan penelitian ini adalah untuk mengetahui perbedaan kadar TNF-Î± saliva dan derajat kesehatan gingiva pada ibu hamil preeklamsia dan ibu hamil tidak preeklamsia. Metode: Penelitian ini merupakan penelitian analitik korelatif. Populasi dalam penelitian ini adalah ibu hamil yang datang ke Poli IGD dan Instalasi Rawat Inap Poli kebidanan RSMH Palembang. Teknik pengambilan sampel Consecutive Sampling dengan minimal sampel berjumlah 30 pasien dan yang diteliti 38 pasien dengan rincian 19 pasien sebagai kasus dan 19 pasien lainnya sebagai kontrol. Sampel diambil berdasarkan kriteria eksklusi dan inklusi pada ibu hamil dengan skor indeks gingiva 3, kemudian dilakukan uji ELISA. Analisis statistik dengan uji parametrik Independent T-test. Hasil: Nilai kadar TNF-Î± saliva kasus 143,27Â±87,20 pg/ml dan kontrol 142,46Â±44,76 pg/ml, p=0.972. Simpulan: Tidak terdapat perbedaan bermakna antara nilai kadar TNF-Î± saliva ibu hamil preeklamsia dan ibu hamil tidak preeklamsia.Introduction: Some theories stated that periodontal disease might increase the risk factors for preeclampsia. Gingivitis can cause elevated levels of the TNF-Î± saliva and may lead to a systemic inflammation and preeclampsia. The purpose of this study was to determine differences in the levels of TNF-Î± saliva and degree of gingival health in pregnant women with and without preeclampsia. Methods: This research was a correlative analytic research. The population in this study was the pregnant women who came to Emergency Department Polyclinics and Midwifery Inpatient Installation Polyclinics of Mohammad Hoesin General Hospital Palembang. The sampling technique used was a consecutive sampling with the minimum sample amounted to 30 patients, and as much as 38 patients were studied with the details of 19 patients as the case group and 19 other patients as the control group. Samples were taken based on the exclusion and inclusion criteria of the pregnant women with gingival index score of 3, then an ELISA test was performed. Statistical analysis was performed using the parametric test of independent T-test. Results: The value of TNF-Î± saliva case was 143.27 Â± 87.20 pg/ml and in the control group was 142.46 Â± 44.76 pg/ml, with p = 0.972. Conclusion: There was no significant difference between the salivary TNF-Î± saliva values of pregnant women with preeclampsia and without preeclampsia.

IFN-GAMMA SECRETION AND IL-10 AFTER STIMULATION OF ESAT-6-CFP-10 (EC610) FUSION ANTIGEN IN PATIENTS WITH ACTIVE TUBERCULOSIS AND LATENT TUBERCULOSIS Prihantika S., Sabrina; Kurniati, Nova; Rahadiyanto, Kemas Ya'kub; Saleh, M Irsan; Hafy, Zen; Tanoerahardjo, Francisca Srioetami; Nugraha, Jusak; Salim, Eddy Mart
Biomedical Journal of Indonesia Vol 5, No 3 (2019)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v5i3.8897

Tuberculosis (TB) is an infectious disease caused by the bacterium Mycobacterium tuberculosis which is still a health problem in the world including in Indonesia. TB infection arises due to interference with the regulation of the body's defense system. Cellular immune system plays a role in fighting TB infection, namely the role of T lymphocytes that differentiate into Th1 cells secrete pro-inflammatory cytokines IFN-? and Th2 cells that secrete IL-10 anti-inflammatory cytokines. The EC610 fusion antigen is specific and has a strong antigenicity to T cell stimulation. The aim of the study was to determine differences in the mean levels of IFN-? and IL-10 after EC610 antigen stimulation in patients with active TB and latent TB. The design of this study was a quasi-in vitro experiment with PBMC culture stimulated by EC610 antigens in the active TB and latent TB groups. The research and examination were carried out at the South Sumatra Province SSR and the Health Research and Development Laboratory Center, Central Jakarta. There were 21 subjects with active TB and 28 subjects with latent TB who met the inclusion criteria. Measurement of IFN-levels? and IL-10 was performed using ELISA-Reader. The results showed the levels of IFN-? and IL-10 after EC610 antigen stimulation were higher in active TB than latent TB. High levels of IFN-? active TB patients showed protective immune responses against M.tb germs while high levels of IL-10 showed their role as anti-inflammatory. There is no significant difference in the average level of IFN-? in patients with active TB and latent TB (p = 0.769) and there was a significant difference in the level of IL-10 levels after EC610 antigen stimulation was higher in active TB than latent TB (p = 0,000).

IFN-GAMMA AND IL-2 SECRETION AFTER ESAT-6-CFP-10 (EC-610) FUSION ANTIGEN STIMULATION FROM PATIENTS WITH ACTIVE LUNG TUBERCULOSIS AND LATENT LUNG TUBERCULOSIS Darwin, Bastian; Kurniati, Nova; Rahadiyanto, Kemas Ya'kub; Saleh, Mgs Irsan; Tanoehardjo, Francisca Srioetami; Nugraha, Jusak; Salim, Eddy Mart
Biomedical Journal of Indonesia Vol 6, No 2 (2020)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v6i2.10741

The Secretion of IFN-Gamma and IL-2 After ESAT-6-CFP-10 Fusion Antigen Stimulation in Active and Latent TB Patients. This study held to discover how immune responses work and to know the pathogenesis of active TB and latent TB patients. This study used PBMC to stimulate T Cells with ESAT-6 and CFP-10 antigen fusion, and measure the level of IFN-gamma and IL-2 with ELISA antibody sandwich (U-Cytech). 16 ml of blood were drawn to 5 tubes. ESAT-6 CFP-20 inducted one tube with QuantiFERON for IFN-gamma assay. The other four tubes were PBMC isolated using Ficoll-Paque, and pre-incubated with stimulation of ESAT-6 CFP-10 fusion antigen for 24-72 hours at 370 C and measured using T-Spot and ELISA reader. We got from this study that there are no significant differences in IFN-gamma levels for both groups with active TB and latent TB. Measurement of IL-2 levels showed significant differences between the two group.

IFN-GAMMA AND IL-2 SECRETION AFTER ESAT-6-CFP-10 (EC-610) FUSION ANTIGEN STIMULATION FROM PATIENTS WITH ACTIVE LUNG TUBERCULOSIS AND LATENT LUNG TUBERCULOSIS Darwin, Bastian; Kurniati, Nova; Rahadiyanto, Kemas Ya'kub; Saleh, Mgs Irsan; Tanoehardjo, Francisca Srioetami; Nugraha, Jusak; Salim, Eddy Mart
Biomedical Journal of Indonesia Vol 6, No 2 (2020)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v6i2.10741

The Secretion of IFN-Gamma and IL-2 After ESAT-6-CFP-10 Fusion Antigen Stimulation in Active and Latent TB Patients. This study held to discover how immune responses work and to know the pathogenesis of active TB and latent TB patients. This study used PBMC to stimulate T Cells with ESAT-6 and CFP-10 antigen fusion, and measure the level of IFN-gamma and IL-2 with ELISA antibody sandwich (U-Cytech). 16 ml of blood were drawn to 5 tubes. ESAT-6 CFP-20 inducted one tube with QuantiFERON for IFN-gamma assay. The other four tubes were PBMC isolated using Ficoll-Paque, and pre-incubated with stimulation of ESAT-6 CFP-10 fusion antigen for 24-72 hours at 370 C and measured using T-Spot and ELISA reader. We got from this study that there are no significant differences in IFN-gamma levels for both groups with active TB and latent TB. Measurement of IL-2 levels showed significant differences between the two group.

Comparison of Serum Interleukin 10 Levels between Leprosy and Non-Leprosy Population Kodrati, Adika; Salim, Eddy Mart; Hafy, Zen
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 21, No 1: January 2021
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v21i1.6514

Leprosy is a chronic granulomatous infectious disease that attacks the peripheral nerves, skin, and other organs. The cytokine IL-10 can deactivate macrophages, inhibit IL-12 production, which also inhibits IFN-Ï’ production. IL-10 directly inhibits CD4+T cells and antigen-presenting cell (APC) function in cells infected withÂ M. leprae. The purpose of this study was to determine the differences in IL-10 levels in leprosy patients and non-patients at dr. Mohammad Hoesin Palembang Hospital. This study used a case-control design for comparing 2 groups. Respondents in this study were 80 people. The case samples were all leprosy patients who were treated at the dr. Mohammad Hoesin Hospital, Palembang for the period of January 16 - February 16, 2019, while the control group was medical personnel and paramedics who worked at the same hospital. The examination was carried out using the ELISA Sandwich method. The results showed that there was a significant difference in IL-10 levels between leprosy patients and non-leprosy leprosy (p=0.000). The average IL-10 level in leprosy patients (13.24 pg/ml) was lower than that in non-patients (40.15 pg/ml). It is concluded that there is a difference in IL-10 levels between leprosy patients and non-leprosy patients in leprosy sufferers are lower than non-leprosy persons.Â

The Correlation of Depression Level with Tumor Necrosis FactorAlpha (TNF-α) Concentration in Uncontrolled Bronchial AsthmaPatients Apriansyah, Muhammad Ali; Putranto, Rudi; Salim, Eddy Mart; Shatri, Hamzah
Jurnal Penyakit Dalam Indonesia Vol. 3, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Introduction. Depression occurs at high rates in people with chronic diseases, including bronchial asthma, with the prevalence of depression approaches 50% in tertiary care asthma clinic. Tumor necrosis factor alpha (TNF-α) is known to play a critical role in the pathogenic mechanism of a number of chronic inflammatory disease, including bronchial asthma and depression. There has not been any research data on the subject in Indonesia. The objective of this study was to investigate the correlation between depressive level and TNF-α level in uncontrolled bronchial asthma. Methods. This was a cross sectional study conducted in 40 patients with uncontrolled bronchial asthma at the allergy immunology clinic outpatient of Dr Moh Hoesin Hospital Palembang, during June 2014 until August 2014. Uncontrolled bronchial asthma was assessed using the Asthma Control Test (ACT) questionnaire, whereas depressive symptoms were assessed by Beck Depression Inventory (BDI) questionnaire, and diagnosis was confirmed by the criteria of the Diagnostic and Statistical Manual for Psychiatry-IV Text Revision (DSM-IV TR) / International Code Diagnose 10 (ICD-10). Serum levels of TNF-α was measured by the method of quantitative enzyme-linked immunosorbent assay (ELISA). Results. The median value of the level of depression and serum TNF- α in this study were 16 (10 - 45) and 4.09 (1.29 - 19.57) pg/mL. There was no significant correlation between depressive level and TNF-α level ( r = -0.265 , p = 0.098 ). Conclusions. There was no significant correlation between depressive level and TNF-α level in uncontrolled bronchial asthma

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