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Correlations between morphological traits and catechin content on tea (Camellia sinensis (L.) O. Kuntze) Syahrian, Heri; Carsono, Nono; Rostini, Neni; Rahadi, Vitria Puspitasari; Sriyadi, Bambang
Jurnal Penelitian Teh dan Kina Vol 18, No 1 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.981 KB) | DOI: 10.22302/pptk.jur.jptk.v18i1.64

Abstract

Catechin content in tea plant is a major character in tea breeding program for a healthy beverage. A tea breeding program for high catechin content maybe will performed by indirect selecting a trait or traits that signi­ficantly correlated with high catechin content. This research was performed to identify mor­phological traits which maybe significantly correlated with high catechin content in tea plant. A randomized complete block design with three replications was used in this experiment using 10 selected clones. The experiment was performed in Gambung Experimental Station ot the Research Institute for Tea and Cinchona, Gambung, West Java. Catechin content, tri­choma density of young leaves, weight of p+2, size of mother leaves, chlorophyll content of mother leaf, stomatal density of mother leaf, mother leaf thickness, and mother leaf angle, were measured. The results indicated that stomatal density and mother leaf angle were significantly correlated. Based on path analysis indirect selection for high catechin content could be performed by selecting stomatal density considering to trichoma density, mother leaf angle and chlorophyll content. In addition, indirect selection for high catechin content could be performed by selecting other leaf angle independently without considering other morphological traits.
The growth test of tea clones (Camellia sinensis) from cross pollination first generation clones Rahadi, Vitria Puspitasari; Syahrian, Heri; Sriyadi, Bambang
Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.399 KB) | DOI: 10.22302/pptk.jur.jptk.v19i2.118

Abstract

Efforts to improve the productivity of tea needed new high yielding clones were obtained from the cross and easily propagated vegetatively. Preliminary testing of the clones  needs to be done to determine the growth of cuttings and rooting properties of tea clones from hybridization which are selected by potential production. This study used a Randomized Complate Block Design with three replications. The treatments consist of 14 clones from crosses and GMB 7 as clone comparison. The data is analyzed by using ANOVA and Scott Knott test with the level of 5%. The observations are intended to look at the growth of roots and shoots by dismantling seedling in polybag predetermined randomly at each observation. The results showed that the clones tested had good growth with a low mortality rate, and easily propagated vegetatively. The S / R ratio also showed shoot growth more faster than the growth of root. This character is advantageous because the most appropriate selection criteria for planting in the field.
Vegetative growth ability of artificial cross pollinated of tea clones Syahrian, Heri; Rahadi, Vitria Puspita; Ruhaendi, Endi; Santoso, Budi
Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.846 KB) | DOI: 10.22302/pptk.jur.jptk.v19i2.119

Abstract

Vegetative propagation is common system that carried out in tea propagation. Seedling propagation was not reliable due to time consuming and phenotypic variability in seed plant. Despite yield potential, good quality, pest and disease resistant characters, the easy of vegetative propagation is very important character in tea breeding process. The aim of this study is to selected clones that resulted by artificial pollination for an ability of vegetative propagation character. Plant materials that tested in this experiment were the seed from the vegetative propagation of 14 clones, and GMB 7 clone as a control. Randomized Block Design was used in this experiment by 3 replications. The data analysis was ANOVA and Scott Knott test. The result of the experiment showed that clone number 41, 45, 38, 149, X5, X3, 122, 78, and 44, were the easiest clones to be vegetative propagated.
Identification of catechin content and its derivates of tea germplasm collection clones Syahrian, Heri
Jurnal Penelitian Teh dan Kina Vol 19, No 1 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.352 KB) | DOI: 10.22302/pptk.jur.jptk.v19i1.95

Abstract

Katekin merupakan salah satu poliphenol utama pada tanaman teh yang menjadikan teh sebagai minuman yang baik untuk dikonsumsi. Program pemuliaan tanaman teh dalam merakit klon yang memiliki kandungan katekin tinggi tidak dapat dilepaskan dari plasma nutfah teh. Informasi akan kandungan katekin klon koleksi plasma nutfah dapat menjadi pegangan bagi pemulia dalam melakukan seleksi tetua untuk proses persilangan buatan. Selain itu klon – klon koleksi plasma nutfah dengan kandungan katekin tinggi dapat dikembangkan langsung sebagai klon anjuran yang dapat meningkatkan kandungan katekin bagi para pekebun. Penelitian ini bertujuan untuk mengetahui kandungan katekin pada klon koleksi plasma nutfah teh. Percobaan dilakukan di Kebun Percobaan Gambung Pusat Penelitian Teh dan Kina, Kabupaten Bandung, Jawa Barat. Ketinggian tempat 1300 mdpl, dengan jenis tanah Andisol. Analisis kandungan katekin dilakukan di laboratorium dengan menggunakan metode yang tertera pada AOAC (1984). Bahan tanaman yang digunakan merupakan klon teh generasi pertama koleksi plasma nutfah sebanyak 10 klon, yaitu klon: Cin 20, Cin 29, Cin 149, RS 1, RB 1, RB 2, PS 354, PS 385, KP 2, SA 73. Karakter yang diamati dalam percobaan ini adalah kandungan katekin total, EGC, ECG dan EGCG. Analisis data yang digunakan adalah analisis keragaman dan uji lanjut menggunakan uji gerombol Scott-Knott. Hasil dari penelitian ini adalah (1) Kandungan katekin total dan derivatnya daiantara klon koleksi aksesi plasma nutfah berbeda-beda; (2) Klon dengan kandungan katekin total tertinggi adalah klon RB 1 dan terendah adalah  klon RS 1dan Cin 149; (3) Klon dengan kandungan EGCG yang tertinggi adalah klon RB 1 dan RB 2 dan terendah adalah klon Cin 149 dan RS 1. (4) Klon dengan kandungan ECG tertinggi terdapat pada klon Cin 149 dan terendah pada klon PS 354 dan Cin 20.
Respon beberapa klon bibit kina (Chinchona sp) asal setek sambung dua spesies di berbagai media tanam Maxiselly, Yudithia; Shohibboniawan, Ahmad; Sutari, Wawan; Wicaksana, Noladhi; Syahrian, Heri
Kultivasi Vol 16, No 3 (2017)
Publisher : Fakultas Pertanian UNPAD

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (863.878 KB) | DOI: 10.24198/kltv.v16i3.14450

Abstract

Kina merupakan tanaman yang selama ini dimanfaatkan sebagai obat berbagai penyakit, seperti malaria dan jantung karena memiliki kandungan alkaloid yang beragam. Peningkatan produktifitas tanaman kina dibutuhkan untuk menunjang kuantitas dan kualitas obat bahan alam. Salah satu factor penting dalam kualitas tanaman kina adalah bahan tanam yang baik. Bahan tanam juga didukung dengan kecocokan media tanam. Media tanam yang selama ini digunakan pada pembibitan kina memiliki kendala di bobot media sehingga sulit untuk pendistribusian bibit. Penelitian ini bertujuan mencari media baru yang cocok untuk bahan tanam kina. Bahan tanam menggunakan 6 klon kina succi yang disambung dengan 1 jenis kina ledger pada 5 media tanam. Metode experiment menggunakan RAK sederhana yang diulang 2 kali. Pengamatan meliputi persentasi hidup, tinggi tanaman, jumlah daun, diameter batang, dan jumlah tunas yang diamati saat bibit kina berumur 3 bulan. Hasil pengamatan menunjukkanterdapat pengaruh pada presentasi hidup dan diameter batang. Klon 1, 4, dan 5 menunjukkan respon baik diberbagai media tanam pada variable tersebut Hasil penelitian ini menunjukkan adanya potensi media yang dapat menggantikan media tanam kina yang selama ini digunakan. Kata Kunci : C.ledgeriana, C.succirubra, Fluff, Topsoil,
DEEP CNNBASED DETECTION FOR TEA CLONE IDENTIFICATION Ramdan, Ade; Suryawati, Endang; Kusumo, R. Budiarianto Suryo; Pardede, Hilman F.; Mahendra, Oka; Dahlan, Rico; Fauziah, Fani; Syahrian, Heri
Jurnal Elektronika dan Telekomunikasi Vol 19, No 2 (2019)
Publisher : Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jet.v19.45-50

Abstract

One factor affecting the quality of tea is the selection of plant material that would be planted on the field. Clonal selection is a common way to produce tea with better quality. However, as a natural cross pollination species, tea often consists of various clones or progenies of cross-pollinated process. This commonly occurs on plantations owned by smallholder farmers. To produce a consistent quality tea, the clones or progenies need to be identified. Usually, human experts distinguish the plants from leaves by visual inspection on the physical attributes of the leaves, such as the textures, the bone structures, and the colors. It is very difficult for non-experts or common farmers to do such identifications. In this, we propose a deep learning-based identification of tea clones. We apply deep convolutional neural network (CNN) to identify 3 types of tea clones of Gambung series, a series of tea clones developed at Research Institute of Tea and Cinchona. Our study indicates that the performance of the CNN systems are affected by the depth of the convolutional layers. VGGNet, a popular CNN architectures with 16 layers, achieves better accuracy compared to AlexNet, a CNN with 6 layers.
In vitro lipase enzyme inhibitory activities of green tea and other herbs Megawati, Megawati; Artanti, Nina; Mulyani, Hani; Darmawan, Akhmad; Syahrian, Heri; Lotulung, Puspa Dewi N.; Supriadi, Edi; Widiyarti, Galuh; Dewi, Rizna Triana; Meilawati, Lia; Ernawati, Teni; Dewijanti, Indah Dwiatmi; Minarti, Minarti
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 9, No 1 (2020)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jgi.9.1.48-52

Abstract

Background: Increased lipase activity can increase the amount of monoglycerides and fatty acids absorbed by the body, this is what affects obesity. If pancreatic lipase activity is inhibited, the production of fatty acids will decrease, consequently the level of fat in the blood will also decrease.Objective: To study anti obesity potential of a single herb or a mixture of green tea and other herbs (Syzygium polyanthum. Lv, Artocarpus communis.Lv, Cinnamomum verum..Lv, Manilkara zapota.Lv, Tectona grandis.Lv) by the in vitro inhibition assay of the lipase enzyme activityMethod: A single (8 herbs) or a mixture of three herbs powder (12 formulas) was put into a tea bag with a total weight of 1.5 g to be brewed in 100 mL bottled mineral water at 70⁰-90⁰C for 10 minutes. In vitro lipase enzyme inhibition assay were conducted to measure the ability of these samples as lipase inhibitor. Orlistat used as positive lipase inhibitor. Results: There was a difference results of lipase inhibition activity between the sample of a single and a mixture of herbs. Interestingly, the results shows that formula 3 (mixture of green tea, Manilkara zapota and cinnamon). has 53.942 % inhibition, formula 9 (a mixture of green tea, Syzygium polyanthum and cinnamon) has 67.322 % inhibition and formula 12 (a mixture of green tea, Tectona grandis and cinnamon) has 56.612 % inhibition which close to lipase inhibitory activity by standard Orlistat.Conclusion: The mixture of green tea and two other herbs has a lipase inhibitory activity similar to Orlistat, when compared to the single herb. The highest lipase inhibitory activity is found in formula 9.