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Analisis Total Plate Count (TPC) Mikroba pada Ikan Asin Kakap di Kota Sorong Papua Barat Sukmawati Sukmawati; Fatimah Hardianti
Jurnal Biodjati Vol 3, No 1 (2018): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i1.2368

Abstract

Ikan Asin kering merupakan salah satu produk perikanan yang banyak diminati oleh masyarakat khususnya di kota Sorong, ditandai dengan tingginya permintaan konsumen. Tingginya permintaan konsumen tidak menjamin kualitas ikan tersebut aman dari cemaran, baik cemaran zat kimia, fisika, dan cemaran mikrobiologi. Produk ikan asin kering harus terjaga dari cemaran agar dapat dikonsumsi dengan aman.  Produk pangan yang aman ialah produk yang  bebas dari bahan pengawet atau bahan kimia yang tidak dianjurkan seperti formalin. Selain itu, jumlah mikroba pada produk tersebut tidak boleh melewati batas standar maksimum nasional Indonesia. Tujuan penelitian ialah untuk menganalisis total plate count mikroba pada ikan asin kakap batu di kota Sorong. Metode yang digunakan adalah metode deskriptif. Hasil analisis data total plate count mikroba pada ikan asin kakap batu (Lutjanus vivanus) secara berturut-turut sebagai berikut; pada faktor pengenceran 10-4- 10-5  mulai dari sampel LG memiliki jumlah koloni 2.36 x 107 – 5 x 107 cfu/g, sampel KB 1.84 x 107 – 5.9 x107, dan sampel KS memiliki jumlah koloni 2.06 x 107- 6.7 x 107. Sehingga dapat disimpulkan bahwa ikan asin kakap batu tersebut tidak layak untuk dikonsumsi, sebab jumlah angka lempeng total (ALT) melewati batas maksimum standar nasional Indonesia.
Isolasi Bakteri Selulolitik dari Limbah Kulit Pisang Sukmawati Sukmawati
Biotropic : The Journal of Tropical Biology Vol. 2 No. 1 (2018): Biotropic, Volume 2, Nomor 1, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.868 KB) | DOI: 10.29080/biotropic.2018.2.1.46-52

Abstract

Cellulose can be found in whole parts of plants. Cellulose can be degraded by cellulose enzyme. Cellulose enzyme is produced by cellulolytic bacteria which has ability to degrade the cellulose and fiber. Cellulose enzyme is extremely important in industry sector. This research aims to isolate the cellulosic bacteria of peel waste of Banana in Sorong, West Papua. This research used isolation and purification method by using 1 gram of Banana peel. It was isolated in CMC (Carboxymethyl cellulose) selective media and morphology characteristic test using 400X-1000X microscope and zone index test. The result showed that the cellulosic ability was found in isolate B with pure zone index 3mm and the smallest pure zone index was in isolate H with pure zone index 0,8 mm. while, the isolate C had zone pure index 1,5 mm, isolate D 1,625 mm, isolate E 1 mm, isolate F 0,714mm, and isolate G 2 mm.
ANALISIS TINGKAT PENGETAHUAN MASYARAKAT DESA AIR SALOBAR TERHADAP BAHAYA RESISTENSI BAKTERI AKIBAT PENGGUNAAN ANTIBIOTIK YANG TIDAK RASIONAL Melda Yunita; Sukmawati Sukmawati
Teknosains Vol 15 No 1 (2021): Januari-April
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v15i1.17684

Abstract

Penyakit infeksi pada masyarakat Indonesia khususnya di Provinsi Maluku, Ambon masih menjadi masalah kesehatan masyarakat yang penting untuk diperhatikan. Penyakit infeksi ini bisa diobati dengan menggunakan antibiotik. Namun penggunaan antibiotik yang tidak rasional dapat menimbulkan penyakit baru pada penderitanya. Tujuan dari penelitian ini adalah untuk menganalisis tingkat pengetahuan masyarakat Desa Air Salobar terhadap bahaya resistensi bakteri akibat penggunaan antibiotik yang tidak rasional. Metode yang digunakan adalah metode deskriptif yaitu dengan memberikan pretest dan posttest kepada masyarakat Desa Air Salobar RT / RW 003/005 Kecamatan Nusaniwe Ambon. Hasil pengabdian masyarakat bahwa baik responden perempuan maupun laki-laki menunjukkan adanya peningkatan pengetahuan tentang bahaya resistensi bakteri akibat penggunaan antibiotik yang tidak rasional. Responden perempuan mengalami peningkatan nilai sebesar 20,55% dari 18 responden, sedangkan untuk responden laki-laki terjadi peningkatan nilai sebesar 32,85% dari 7 responden. Total rata-rata tingkat peningkatan pengetahuan tentang bahaya resistensi bakteri akibat penggunaan antibiotik yang tidak rasional adalah 24% dari total jumlah responden.
Analysis of hemolysis activity of pathogenic bacteria on salted Lutjanus vivanus at Remu traditional market, Sorong city Sukmawati Sukmawati; Cikita Putri Tania Tindoy; Sipriyadi Sipriyadi
Bioscience Vol 6, No 1 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202261114211-0-00

Abstract

Fish is one of the fishery food ingredients needed by humans because fish meat has compounds that are needed by the body. These compounds consist of proteins, fats, carbohydrates, vitamins and mineral salts. Indonesia is an archipelagic country that has a lot of potential in agriculture and fisheries, and Sorong, West Papua, is no exception. The results of people's fisheries in Sorong, West Papua Province are quite high. Based on data from the Sorong City Fisheries Service, there are five types of fish that are exported abroad, such as China, Korea, and Japan. These types of fish include tuna, mackerel, bubarah fish, red snapper, and grouper or rock snapper. Stone snapper is not only consumed in the form of fresh fish, fish collectors also process it into dried salted fish. The purpose of this study was to analyze the hemolysis activity of pathogenic bacteria in salted snapper in the Remu market, Sorong city. This study is a descriptive study by providing a description of the object under study, namely the analysis of the hemolytic activity of pathogenic bacteria in salted rock snapper (Lutjanus vivanus) originating from the Remu traditional market in Sorong city. The conclusion of the research that has been carried out is that the hemolytic index of pathogenic bacteria for each sample shows the hemolytic index area from the highest to the lowest, sequentially from the untreated sample, namely the sample Do (110.0 mm), Ko (116.6 mm), To (453.3 mm), samples that were treated with frying before isolation were samples Dg (52.5 mm), Kg (3.5 mm), Tg (40.0 mm), while the smallest hemolytic index value was the sample that was boiled before The isolated samples were Dm (41.6 mm), Km (3.5 mm), and Tm samples (18.3 mm). Furthermore, all test samples showed β- hemolytic or total hemolytic.Ikan sangat dibutuhkan manusia sebab daging ikan memiliki senyawa-senyawa yang sangat dibutuhkan oleh tubuh. Senyawa-senyawa tersebut terdiri dari protein, lemak, karbohidrat, vitamin dan garam-garam mineral. Indonesia merupakan negara kepulauan yang memiliki banyak potensi dalam bidang pertanian maupun bidang perikanan, tidak terkecuali di Sorong Papua Barat. Hasil perikanan rakyat di Sorong Provinsi Papua Barat cukup tinggi. Berdasarkan data dari Dinas Perikanan Kota Sorong ada lima jenis ikan yang di ekspor keluar negeri seperti china, Korea, dan jepang. Jenis ikan tersebut diantaranya ialah ikan tuna, ikan tenggiri, ikan bubarah, ikan kakap merah, dan ikan kerapuh atau kakap batu. Ikan kakap batu selain dikonsumsi dalam bentuk ikan segar, para pengepul ikan juga mengolahnya ke dalam bentuk ikan asin kering. Adapun tujuan dari penelitian ini ialah untuk menganalisis aktivitas hemolysis bakteri pathogen pada ikan asin kakap batu di pasar Remu kota Sorong. Penelitian ini merupakan penelitian deskriptif dengan memberikan penggambaran tentang objek yang diteliti yaitu analisis aktivitas hemolisis bakteri patogen pada ikan asin kakap batu (Lutjanus vivanus) yang berasal dari pasar Remu kota Sorong. Adapun kesimpulan dari penelitian yang telah dilaksanakan ialah indeks hemolitik bakteri patogen untuk tiap sampel berturut-turut menunjukkan luas indeks hemolitik dari yang tertinggi hingga ke rendah, berturut-turut mulai dari sampel yang tidak diberikan perlakuan yakni sampel Do (110.0 mm), Ko (116.6 mm), To (453.3 mm), sampel yang diberi perlakuan penggorengan sebelum isolasi ialah sampel Dg (52.5 mm), Kg (3.5 mm), Tg (40.0 mm), sedangkan nilai indeks hemolitik yang paling kecil ialah sampel yang diberi perlakuan rebus sebelum diisolasi yaitu sampel Dm (41.6 mm), Km (3.5 mm), dan sampel Tm (18.3 mm). Selanjutnya seluruh sampel uji menunjukkan sifat β-hemolitik atau hemolitik total.
Identify Of Floc-Forming Bacteria In Shrimp Pond In Pangkep District Sukmawati Sukmawati
Bioscience Vol 1, No 2 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.613 KB) | DOI: 10.24036/02017128060-0-00

Abstract

This research aims to identify the kinds of floc-forming bacteria in shrimp pond in Pangkep District. This study was a descriptive research. Floc-forming bacteria were isolated from the water and mud samples. According to microbial technique isolation procedure, by using Nutrient Agar (NA) as isolation medium. The result of this study showed that there were 37 isolates. And Then floculated activity was measured: The result showed the theme 5 isolates which have more than 79% of floculating activity namely, isolat 1,isolat 2, isolat 3, isolat 4, isolat 5, floculating activity: 90,34%, 90,34 %, 94,03%, 79,68%, 92,47% respectively. At can be cocluded that isolat 1. Enterococcus sp, isolat 2. Bacillus spdan isolat 3. Vagococcus sp..Keywords: Identification, floc-formingbacteria,shrimp pond
The Effect of N-Hexan Extract of Sambiloto Leaf (Andrographis paniculata) on Reproduction Function of Female ICR of Mice (Mus musculus) Period of Praimplantation and Pascaimplantation Muliana Muliana; Sukmawati Sukmawati
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.165 KB) | DOI: 10.24036/0201931103252-0-00

Abstract

Abstract. The objective of this research is to know the effect of giving of n-hexan extract of A. Paniculata leaf on the reproduction function on female mice and when hypodermic of how many dose of extract n-hexan most having an effect on the reproduction function on female mice. Extract gift conducted by inoculation. Mice or test animal obtained from Hall of Animal Research (BPH) of Regency Maros. Old age the mencit 10-12 week of weighing of mean body 21g, while test substance used by leaf of Andrographis paniculata obtained in regency of Bone of subdistrict of Sibulue of countryside of Pattiro Bajo. Parameter perceived that is s the number of implantation, number of life fetus, gestation loss, dead foetus, embryo resorbsi, corpus luteum, and death pascaimplantasi. This Research represent the research of experiment and desain research used that is Complete Random Device ( RAL) consisted of by three treatment group five times is restating so that obtained by fifteen combination. Result of research indicate that the gift of n-hexan extract of A. Paniculata leaf at dose level tested degrade the number of implantation, number of life fetus, improving number of gestation loss, and number of died fetus , also generate the effect fitotoksik of body weight of mice mains. From inferential research result that extract of n-hexan A. Paniculata leaf can effect on the reproduction function on female mice with the hypodermic time most having an in with day of pregnancy to-0 until pregnancy day to-4.
Identification and Characterization of PrTK 2 Bacterial Isolate Producing Extracelular Protease Enzym From Tempeh Rubber Seeds Cico Jhon Karunia Simamora; Sukmawati Sukmawati
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.805 KB) | DOI: 10.24036/0202041108255-0-00

Abstract

Protease is an enzyme that can hydrolyze proteins into simpler compounds such as small peptides and amino acids. The enzymes produced by microorganisms can be isolated by separating cells by centrifugation. This study aims to determine the biological characteristics of the PrTK 02 bacterial isolate and the characteristics of the crude extract of the protease enzyme produced by the PrTK 02 bacterial isolate. The study was conducted by inoculating 1 ose PrTK 02 bacteria on the Nutrient Broth (NB) media. Pre-culture was carried out at 37 °C for 48 hours with an agitation speed of 120 rpm. Protease enzyme extraction was carried out by centrifugation of bacterial growth media at a speed of 3000 rpm for 15 minutes at 4 °C. Supernatant as a sample was tested for protease activity and protein content. The results of this study were obtained from the protease enzyme from bacterial isolate PrTK 02 marked by a clear zone in the medium of high school and the highest activity at the time of production 24 hours with the specific activity of the enzyme 1.2154 unit / mg protein. Biochemical and microscopic identification shows that the isolate of PrTk 02 bacteria is a class of Bacillus sp. PrTk 01 which is a Gram-positive bacteria that is found in tempeh.
TOTAL MICROBIAL PLATES ON BEEF AND BEEF OFFAL Sukmawati Sukmawati
Bioscience Vol 2, No 1 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.178 KB) | DOI: 10.24036/02018219825-0-00

Abstract

Efforts to improve people’s nutritional health can be obtained through nutritional food. Beef is a source of animal protein needed. But foodstuffs such as meat and eggs other than as a source of protein is also one of the microorganisms breeding media, which can be a type of human disease. The aim this study was to find out whether the sample of beef, beef heart, and beef liver are safe to consume and marketable. This research method includes tool sterilization, microbial growth medium, and the analysis phase used total plate count method. Samples of beef number of microbial colonies as much as 660 cells/ml. samples of cow’s heart contained colonies 3.150 cells/ml, and in liver samples there are 3.000 cells/ml. so it can be concluded that beef and dairy products are feasible to marketable and to consumption. Keywords: Total Microbial, Beef, Beef Offal
Analysis of Hedonic Test and Total Microbial Plate Numbers on Fish Siomay in Sorong City West Papua Sukmawati Sukmawati
Bioscience Vol 4, No 2 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202042108256-0-00

Abstract

One of the Indonesian snacks that can be found in all regions is fish siomay. fish siomay in the city of Sorong, there have been no reports on both the level of community preference and food security. The purpose of this study was to determine the level of community preference and the total number of microbial plate numbers snacks on fish siomay in the city of Sorong, West Papua. The method used in this research is descriptive method and experimental method. The results showed that for the hedonic test had a varying degree of preference for each sample, while the total number of plates did not exceed the maximum limit, then for the correlation of the two tests showed there was a relationship. The hedonic test results from the five samples show the best level of preference is the BS sample, and the EH sample, then the AQ sample, and the DS sample and the lowest preference level is the CR sample. While the sample of fish siomay scattered in the city of Sorong was suitable for consumption. 
Screening of Probiotic Bacteria Candidates in The Mangrove Tourism Area in Klawalu Sorong City West Papua Sukmawati Sukmawati; M Iksan Badaruddin
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.608 KB) | DOI: 10.24036/0201932105397-0-00

Abstract

Probiotic bacteria was one of the biological control agents (biological control). It had a role in suppressing or killing pathogenic microorganisms. In addition, it also played a role in improving water quality as in fish maintenance media. The basic principle of probiotics was the utilization of the ability of microorganisms to increase absorption in the digestive tract of fish. Probiotic bacteria were able to produce antimicrobial compounds such as lactic acid, hydrogen peroxide, bacteriocin. These compounds were antimicrobial and antibiotic that can suppress the growth of pathogenic bacteria. The purpose of this study was to detect candidate probiotic bacteria from the mangrove tourism area in Klawalu, Sorong City, West Papua. The results of screening for probiotic bacteria candidates by using selective media, from 16 samples consisting of water samples and mud samples’ obtained 11 positive samples, there were probiotic bacteria candidates with the bacterial morphological characteristics of each colony was almost similar. Keywords: Probiotic Bacteria, Mangrove Land, Sorong City