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All Journal ACTA VETERINARIA INDONESIANA International Journal of Integrated Health Sciences Althea Medical Journal Indonesian Journal of Cancer Chemoprevention Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat The Indonesian Biomedical Journal Oto Rhino Laryngologica Indonesiana
Gita Widya Pradini
Departemen Ilmu Kedokteran Dasar, Fakultas Kedokteran Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Engineering Health Professions Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health Veterinary

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Destructive Effect of Calcium Hypochlorite against Pseudomonas aeruginosa Biofilm Arifani, Ilma; Pradini, Gita Widya; Desy Arya, Insi Farisa; Cahyadi, Adi Imam
Althea Medical Journal Vol 4, No 3 (2017)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (681.179 KB) | DOI: 10.15850/amj.v4n3.1205

Abstract

Background: Pseudomonas aeruginosa is the most common bacteria contaminating the hemodialysis water and has high capability to form a biofilm. The presence of biofilm is hazardous because it becomes a constant source of bacterial and toxin release toward the hemodialysis patient’s blood. Calcium hypochlorite (Ca(OCl)2) is an easily obtained disinfectant. This study was aimed to detect the destructive effect of Ca(OCl)2 against P. aeruginosa biofilm and the optimal disinfectant concentration required to achieve significant effect.Methods: This experimental study was conducted in six replicates from September to October 2015 in Microbiology Laboratory of Faculty of Medicine Universitas Padjadjaran Bandung. A modified tissue culture plate method was performed to grow P. aeruginosa biofilms which were subsequently treated with Ca(OCl)2 in various chlorine concentrations, namely 20, 30, 40, and 500 parts per million (ppm). The data was analyzed using Welch Analysis of Variance (ANOVA) and Games-Howell post-hoc tests and presented in tables.Results: Data were obtained from 36 flat-bottomed polystyrene wells. There was a statistically significant mean difference between groups [F(4, 11.92)= 91.198, p<0.001)]. All of the tested chlorine concentrations caused significant decreases in biofilm optical densities (p = 0.027 for 20 ppm and p< 0.001 for 30, 40, and 500 ppm).Conclusions: Ca(OCl)2 with chlorine concentrations of 20, 30, 40, and 500 ppm have significant destructive effect against P. aeruginosa biofilm. The mean differences among treated groups were not significant. The most optimum concentration is 30 ppm.
KUALITAS MIKROBIOLOGI SUSU KAMBING SEGAR DARI SEBUAH PETERNAKAN KAMBING PERAH SKALA KECIL DI DESA CIMALAKA, SUMEDANG Gita Widya Pradini; Nisa Fauziah; Rini Widyastuti; Mas Rizky Anggun Adipurna Syamsunarno
Dharmakarya Vol 10, No 2 (2021): Juni, 2021
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/dharmakarya.v10i2.24991

Abstract

ABSTRAK. Susu kambing merupakan salah satu sumber susu yang dipercaya memiliki banyak manfaat oleh masyarakat. Dalam praktik penjualannya, sebagian besar peternak skala kecil di Indonesia tidak melakukan proses sterilisasi atau pasteurisasi susu. Adanya kepercayaan masyarakat bahwa susu kambing mentah lebih berkhasiat menjadi perhatian akan risiko penularan mikroba patogen. Kegiatan ini bertujuan untuk mengetahui kualitas mikrobiologi susu kambing mentah dari sebuah peternakan kambing perah di Desa Cimalaka serta memberikan penyuluhan dan survey kepada masyarakat dan peternak kambing perah di desa tersebut. Dari salah satu peternakan, didapatkan 6 ekor kambing perah peranakan Etawah. Setiap kambing dilakukan pemerahan susu sebanyak ±10ml. Sampel susu kemudian dinilai kualitas mikrobiologi berdasarkan hasil perhitungan angka lempeng total (ALT) serta identifikasi bakteri Staphylococcus koagulase positif dan bakteri koliform. Batas maksimal jumlah bakteri dalam ALT ditentukan berdasarkan Standar Nasional Indonesia (SNI) 1788:2009. Dalam kegiatan penyuluhan terdapat 20 responden yang mengikuti survey, 14 di antaranya merupakan peternak kambing perah. Hasil pemeriksaan mikrobiologi menunjukkan seluruh sampel susu memenuhi kriteria SNI berdasarkan ALT. Pertumbuhan bakteri koliform tidak ditemukan pada seluruh sampel. Ditemukan cemaran Staphylococcus koagulase positif pada dua sampel dan cemaran bakteri enterik non-koliform pada dua sampel lainnya yang berpotensi sebagai patogen. Hasil survey menunjukkan sebagian besar responden terbiasa mengonsumsi susu mentah, dan praktik peternak dalam menjaga sanitasi kandang dan higienitas susu masih belum baik Temuan ini menunjukkan pentingnya peningkatan pengetahuan dan praktik peternak dalam manajemen hewan ternak dan susu segar. Praktik meminum susu kambing mentah perlu dihindari karena berisiko tinggi terkontaminasi mikroba patogen.
Destructive Effect of Calcium Hypochlorite against Pseudomonas aeruginosa Biofilm Ilma Arifani; Gita Widya Pradini; Insi Farisa Desy Arya; Adi Imam Cahyadi
Althea Medical Journal Vol 4, No 3 (2017)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (681.179 KB) | DOI: 10.15850/amj.v4n3.1205

Abstract

Background: Pseudomonas aeruginosa is the most common bacteria contaminating the hemodialysis water and has high capability to form a biofilm. The presence of biofilm is hazardous because it becomes a constant source of bacterial and toxin release toward the hemodialysis patient’s blood. Calcium hypochlorite (Ca(OCl)2) is an easily obtained disinfectant. This study was aimed to detect the destructive effect of Ca(OCl)2 against P. aeruginosa biofilm and the optimal disinfectant concentration required to achieve significant effect.Methods: This experimental study was conducted in six replicates from September to October 2015 in Microbiology Laboratory of Faculty of Medicine Universitas Padjadjaran Bandung. A modified tissue culture plate method was performed to grow P. aeruginosa biofilms which were subsequently treated with Ca(OCl)2 in various chlorine concentrations, namely 20, 30, 40, and 500 parts per million (ppm). The data was analyzed using Welch Analysis of Variance (ANOVA) and Games-Howell post-hoc tests and presented in tables.Results: Data were obtained from 36 flat-bottomed polystyrene wells. There was a statistically significant mean difference between groups [F(4, 11.92)= 91.198, p<0.001)]. All of the tested chlorine concentrations caused significant decreases in biofilm optical densities (p = 0.027 for 20 ppm and p< 0.001 for 30, 40, and 500 ppm).Conclusions: Ca(OCl)2 with chlorine concentrations of 20, 30, 40, and 500 ppm have significant destructive effect against P. aeruginosa biofilm. The mean differences among treated groups were not significant. The most optimum concentration is 30 ppm.
Prevalence of Trichomonas vaginalis Based on Clinical Manifestation and Polymerase Chain Reaction among Reproductive Women Huriyah Hafizhotul &#039;Ummah; Gita Widya Pradini; Reiva Farah Dwiyana; Muhammad Ersyad Hamda
International Journal of Integrated Health Sciences Vol 7, No 1 (2019)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/ijihs.v7n1.1490

Abstract

Objective: To measure the prevalence of Trichomonas vaginalis (T. vaginalis) based on clinical manifestations and polymerase chain reaction (PCR) among reproductive women.Methods: Subjects of the study were the vaginal swab obtained from reproductive women who attended the gynecology examination at Kandanghaur and Sindang primary health care of Indramayu District, West Java in 2016. This study was a descriptive study with cross-sectional method. Sampling was performed with total sampling method and 76 of vaginal swabs were included in this study. The prevalence of T. vaginalis was measured using PCR. The vaginal specimens were collected and then processed for PCR analysis using TVK3/TVK7.Results: Prevalence of T. vaginalis among reproductive women in Indramayu District, West Java that analyzed using PCR was 0%. This result could be affected by the study setting in community, presence or absence of symptoms, and population studied.Conclusion: There were no positive results of T. vaginalis, suggested by the samples that obtained from community-based of a low-risked population.Keywords: Trichomonas vaginalis, polymerase chain reaction, prevalence, reproductive women 
Phylogeny of HPV-16 and HPV-18 Multiple Infection of a Patient with Cervical Cancer from Dr. Hasan Sadikin General Hospital, Bandung: A Case Report Vera Amalia Lestari; Ika Agus Rini; Gita Widya Pradini; Edhyana Sahiratmadja; Herman Susanto
The Indonesian Biomedical Journal Vol 10, No 3 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i3.383

Abstract

BACKGROUND: From all of human papillomaviruses (HPV) genotypes capable of causing cervical cancer, it is estimated that 70 percent are HPV-16 and HPV-18. HPV-16 can infect the tissues in single infection or together with other high-risk types of HPV, and the most common is with HPV-18. The origin of HPV can be identified by its phylogenetic tree. The aim of this study was to determine the phylogeny of HPV-16 and HPV-18 multiple infection in cervical cancer, whether both HPVs were from the same origin.METHODS: Cervical tissue biopsies (n=33) were obtained from Hasan Sadikin Hospital in the period of September to November 2016. HPV genotyping test was performed to confirm the HPV-16 and HPV-18 multiple infection. L1 gene of both HPV-16 and HPV-18 were sequenced for phylogenetic analysis.RESULTS: Phylogenetic analysis of L1 HPV-16 and HPV-18 showed the closest relationship with sequence from China and Thailand, respectively.CONCLUSION: HPV-16 and HPV-18 multiple infection of a cervical cancer patient from Dr. Hasan Sadikin General Hospital Bandung showed a very close L1 phylogeny relationship with isolate from Asian region.KEYWORDS: HPV-16, HPV-18, multiple infection, cervical cancer, Bandung
Phylogenetic Analysis of Human Papillomavirus 16 and 52 L1 Gene from Cervical Cancer in Bandung, Indonesia Mutia Latief; Ika Agus Rini; Gita Widya Pradini; Gatot Nyarumenteng Adhipurnawan Winarno; Edhyana Sahiratmadja; Herman Susanto
The Indonesian Biomedical Journal Vol 10, No 1 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i1.296

Abstract

BACKGROUND: Chronic infection with high-risk type of human papillomavirus (HPV) can cause cervical cancer. Previous studies showed that multiple infections of HPV are found in cervical cancer caused by multiple HPV infections and the most common are HPV-16 and HPV-52. The origin of HPV-16 circulating in Indonesia varies. The purpose of this study was to explore the origin of multiple infections of HPV-52 and HPV-16 in cervical cancer by using a phylogenetic tree.METHODS: During July-November 2010, 100 women were diagnosed with cervical cancer in the Department of Obstetrics and Gynecology, Dr. Hasan Sadikin General Hospital, Bandung, Indonesia. Only 96 patients were involved in this study. Ninety-six samples of HPV deoxyribonucleic acid (DNA) were isolated from biopsied tissue of cervical cancer. Multiple infections of HPV genotypes HPV-16 and HPV-52 were confirmed by using the linear assay for HPV genotyping test. Afterward,HPV-52L1 gene was amplified by using self-designed primer. L1 gene was also sequenced and analyzed using phylogenetic program (MEGA6.06).RESULTS: The result of phylogenetic tree construction showed that isolated HPV-52 originated from multiple infections of HPV-16 and HPV-52 from cervical cancer patients in Bandung were in a subgroup with isolates originating from EU077219 Canada (America) and KT799980 southwest China (Asia). Isolate HPV-16 in one subgroup with isolates originating from KU951191.1 (Southwest China).CONCLUSION: L1 gene sequence from multiple infections isolated from HPV-16 and HPV-52 from cervical cancer patients in Bandung refers to the variation of L1 gene reported from Canada and southwest China. This proves that Indonesia’s HPV clusters are located in the strains found in America and Asia.KEYWORDS: multiple infections, HPV-16, HPV-52, L1 gene, phylogenetic
Generating Paclitaxel-Resistant in Cervical Cancer HeLa Cell Line Muhammad Hasan Bashari; Fachreza Aryo Damara; Isna Nisrina Hardani; Gita Widya Pradini; Tenny Putri; Eko Fuji Ariyanto
Indonesian Journal of Cancer Chemoprevention Vol 11, No 2 (2020)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev11iss2pp90-96

Abstract

Cervical cancer is one of the most leading causes of women death. Currently, paclitaxel is still one of the main therapeutic regimens for cervical cancer patients. However, some patients developed to be paclitaxel-resistant. Hence, studies to find out the novel strategies to resolve this problem are important. Generating resistant cancer cell lines can be utilized as the potent tool to evaluate the efficacy of any therapeutic agent toward cancer drug-resistant problems. Current studies describing the methods to establish chemoresistance are lacking. Moreover, study in Indonesia conducting chemoresistance in cell line is limited. This study was aimed to elaborate the characteristics of HeLa cells during generation of paclitaxel-resistant cervical cancer cells. The parental HeLa cells were exposed to an escalating concentration of paclitaxel for a long time period. Subsequently, cells were divided into two groups for the evaluation of resistance characteristics. The values of inhibitory concentration 50 (IC50) and inhibitory concentration 90 (IC90) were analyzed using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Our data showed that the longer exposing periods of paclitaxel, the higher IC50 and IC90 values of HeLa cells are. IC90 of paclitaxel in HeLa Pac RB was increased from 69 pM, 440 pM, 2,561 pM and 10,337 pM on 0th, 1st, 2nd, 3rd and 4th months, respectively. Interestingly, the resistant cells were recovered to be paclitaxel-sensitive when they were not being continuously exposed to paclitaxel. In addition, the paclitaxel resistant cells become less sensitive against 5-FU but not doxorubicin, cisplatin and etoposide. We were able to generate cervical cancer HeLa paclitaxel-resistant cell line. These cell line could potentially be utilized for further studies in order to understand the molecular mechanisms of drug resistance in cervical cancer and as a tool for cancer drug discovery.Keywords: cervical cancer, drug resistant cell line, paclitaxel resistant cells, stepwise escalating concentration.
Microbiota profile in sinonasal mucosa of chronic rhinosinusitis as an indicator for therapeutic outcome Imam Megantara; Muhammad Fadhil Ihsan Yazid; Gita Widya Pradini; Melati Sudiro; Chrysanti Murad
Oto Rhino Laryngologica Indonesiana Vol 51, No 2 (2021): VOLUME 51, NO. 2 JULY - DECEMBER 2021
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32637/orli.v51i2.423

Abstract

ABSTRACTBackground: Chronic rhinosinusitis (CRS) is characterized by persistent inflammation of the sinonasal mucosa, which often requires surgical and additional post-operative therapy. Microbial dysbiosis due to the imbalance of commensal bacteria and pathogens plays a crucial role in the inflammatory process within the sinonasal mucosa and in therapy outcome. Further knowledge about the microbiota profile in CRS is needed to improve the management strategy for CRS patients. Purpose: To review recent studies on the microbiota profile in sinonasal mucosa of CRS patients, and its potential as an indicator for therapeutic outcome. Literature review: Recent data from several studies has documented increased microbiota richness and diversity in post-operative CRS patients’ sinonasal mucosa, as well as good post-operative outcomes. Increased genus Corynebacterium abundance was also consistently associated with good post-operative outcomes. Conclusion: There was a difference in microbiota profiles in pre and post-operative CRS patients, indicating an association with clinical improvement. Corynebacterium was associated with better post-operative outcomes. Thus, the presence of these bacteria potentially could be used as an indicator for therapeutic outcome.Keywords: chronic rhinosinusitis, microbiota, endoscopic sinus surgery, therapeutic outcomeABSTRAKLatar belakang: Rinosinusitis kronis (RSK) merupakan kondisi inflamasi yang menetap pada mukosa sinonasal dan sering memerlukan operasi dan terapi tambahan pasca-operasi. Kondisi disbiosis mikrobiota akibat ketidakseimbangan bakteri komensal dan patogen diduga memengaruhi proses inflamasi pada mukosa sinonasal dan luaran terapi. Diperlukan pengetahuan tentang profil mikrobiota pada RSK sehingga diharapkan dapat meningkatkan strategi penanganan pasien RSK. Tujuan: Mempelajari studi terkini tentang profil mikrobiota pada mukosa sinonasal pasien RSK, dan potensinya sebagai indikator luaran terapi. Tinjauan pustaka: Data terkini dari beberapa penelitian menemukan peningkatan kekayaan dan keragaman mikrobiota pada mukosa sinonasal pasien RSK pasca-operasi dan memiliki hubungan dengan luaran operasi yang baik. Peningkatan kelimpahan genus Corynebacterium ditemukan secara konsisten berkaitan dengan luaran operasi yang baik. Kesimpulan: Terdapat perbedaan profil mikrobiota pada pasien RSK pra dan pasca-operasi yang mengindikasikan kaitannya dengan perbaikan klinis. Corynebacterium adalah bakteri yang ditemukan memiliki hubungan dengan luaran operasi yang lebih baik, sehingga keberadaan bakteri ini berpotensi untuk dapat dimanfaatkan sebagai indikator luaran terapi.Kata kunci: rinosinusitis kronis, mikrobiota, operasi sinus endoskopik, luaran terapi
Mekanisme Resistensi Antibiotik pada Lactobacillus dan Potensinya untuk Mengatasi Salmonellosis pada Ayam Broiler Salsabilla Fasya Khoerunnisa; Roostita Lobo Balia; Gita Widya Pradini
Acta VETERINARIA Indonesiana Vol. 10 No. 2 (2022): Juli 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.2.111-123

Abstract

Ayam broiler harus dipastikan terhindar dari penyakit zoonosis, salah satunya Salmonellosis. Resistensi Salmonella sp. terhadap berbagai antibiotik menjadi salah satu alasan dalam penggunaan probiotik sebagai pilihan alternatif untuk mengatasi Salmonellosis. Lactobacillus sp. umum digunakan sebagai probiotik untuk menanggulangi Salmonellosis pada ayam broiler. Lactobacillus sp. dapat menerima agen resisten terhadap antibiotik, baik dari lingkungan maupun di saluran pencernaan ayam. Lactobacillus sp. dapat menjadi reservoir dalam penyebaran agen resisten antibiotik. Berdasarkan hasil studi literatur, Lactobacillus sp. mampu melakukan CE (competitive exclusion) sehingga dapat mengurangi populasi bakteri patogen. Lactobacillus sp. yang diambil dari biakan, saluran pencernaan, feses, daging serta sumber air di sekitar peternakan dan rumah potong unggas memiliki sifat resisten terhadap antibiotik vankomisin, kloramfenikol, tetrasiklin dan eritromisin. Lactobacillus sp. bertindak sebagai pendonor maupun penerima gen resisten terhadap antibiotik kloramfenikol, tetrasiklin dan eritromisin yang disebarkan secara horizontal melalui konjugasi plasmid dan transposon. Lactobacillus sp. yang teridentifikasi resisten terhadap antibiotik vankomisin memiliki potensi transfer gen resisten yang rendah dikarenakan sifat resisten terhadap vankomisin merupakan resistensi instrinsik sehingga secara tidak langsung sifat tersebut sangat kecil kemungkinannya dapat disebarkan secara horizontal kepada mikroorganisme lainnya.
Co-Authors Adi Imam Cahyadi Arifani, Ilma Bashari, Muhammad Hasan Chrysanti Murad Edhyana Sahiratmadja Edhyana Sahiratmadja Eko Fuji Ariyanto Fachreza Aryo Damara Fauziah, Nisa Gatot Nyarumenteng Adhipurnawan Winarno Herman Susanto Herman Susanto Huriyah Hafizhotul &#039;Ummah Ika Agus Rini Ika Agus Rini Ilma Arifani Imam Megantara Insi Farisa Desy Arya Isna Nisrina Hardani Mas Rizky A.A. Syamsunarno Melati Sudiro Muhammad Ersyad Hamda Muhammad Fadhil Ihsan Yazid Mutia Latief Reiva Farah Dwiyana Rini Widyastuti Roostita L Balia Salsabilla Fasya Khoerunnisa Tenny Putri Vera Amalia Lestari