Berry Juliandi
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Bogor Agricultural University (IPB University), Kampus IPB Dramaga, Bogor 16680

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Permanent flame-blunted monofilament of middle cerebral artery occlusion technique for ischemia stroke induction in animal models Ramli, Yetty; Alwahdy, Ahmad S.; Kurniawan, Mohammad; Juliandi, Berry; Wuyung, Puspita E.; Susanto, Yayi D.B.
Medical Journal of Indonesia Vol 26, No 3 (2017): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.83 KB) | DOI: 10.13181/mji.v26i3.1645

Abstract

Background: Rat is the most frequently used animal for ischemic stroke studies. Recently, middle cerebral artery occlusion (MCAO) by introducing various types of surgical monofilament intraluminally has been widely used, with their advantages and disadvantages. For permanent occlusion, problems with mortality in rats are higher than transient. In this study, we used permanent occlusion using modified monofilament by flaming on its tip which may reduce mortality rate, so that chronic phase of stroke can be learned extensively.Methods: Three male Sprague-Dawley rats underwent permanent MCAO. The flame-blunted monofilament was introduced through common carotid artery. Hematoxylin eosin histopathology confirmation and functional assessment post-stroke induction were then evaluated.Results: Evaluation was conducted on 3 rats in different time post-stroke induction (48 hours, 72 hours, and 3 weeks). Using histopathological examination, the infarction was proved in all 3 rats showing red neurons, perivascular edema and neutrophil spongiosis, in infarct and peri-infarct area. The changes in histopathology showed spongiosis were more dominant in 3 week-post-MCAO rats. On the other hand, red neurons and perivascular edema were less compared to 48 and 72-hour-post-MCAO rats.Conclusion: Flame–blunted monofilament showed its efficacy in producing infarct area. The advantages of this technique are easy to perform with simple and less expensive modification of the monofilament. Conducting successful permanent occlusion with less mortality rate will give chances to do further research on stroke in chronic phase and its effect on novel treatment.
Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium Budiariati, Vista; Budiono, Dwi; Fahrudin, Mokhamad; Juliandi, Berry; Rinendyaputri, Ratih; Boediono, Arief
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.143 KB) | DOI: 10.4308/hjb.27.2.89

Abstract

Parthenogenesis is an artificial oocytes activation process without paternal contribution. Blastocyst, derived from parthenogenesis, is one of potential source for pluripotent stem cell propagation. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate. One of the component that predicted inhibit parthenogenetic embryo development is phosphate. Therefore, we try to modify culture medium in order to overcome that problem. The aim of this research was to produce and analyze the characteristics of parthenogenetic blastocyst developed in phosphate-free medium. Mouse oocytes obtained from adult female DDY by superovulation. The activator was strontium chloride 10 mM and diploidization with cytochalasin B 5 μg/ml. Medium for activation and culture medium were modified rat 1 cell embryo medium (MR1ECM) which is phosphate free. The results showed that parthenotes that were cultured in phosphate free medium reached higher blastocyst rate compared to the other groups. The increase of phosphate in culture medium lead to impaired parthenogenetic embryos development. Further experiment was made to analyze the differences between fertilized and parthenogenetic embryo in this medium. The experiment showed that diploid parthenogenetic could achieve high blastocyst rate (30.9±1.3%). The quality of diploid parthenogenetic blastocyst, based on cells number, viability, and ICM ratio, was lower than fertilized blastocyst.
Intravenous Versus Intraarterial Transplantation of Human Umbilical Cord Blood Mononuclear Cells for Brain Ischemia in Rats Yetty Ramli; Ahmad Sulaiman Alwahdy; Mohammad Kurniawan; Berry Juliandi; Puspita Eka Wuyung; Yayi Dwina Bilianti Susanto
HAYATI Journal of Biosciences Vol. 24 No. 4 (2017): October 2017
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1838.272 KB) | DOI: 10.4308/hjb.24.4.187

Abstract

Cerebral ischemia is among the most common type of stroke seen in patient. Regeneration of death neurons remains questionable. Human umbilical cord blood mononuclear cell(cbMNC) is one of the treatment options for ischemia stroke through their various advantages; availability, pluripotency, and immaturity. One group of healthy rats and three groups (n = 6 per group) of male Wistar rats undergone permanent middle cerebral artery occlusion (MCAO). Rats were allowed to recover for 7 days before intraarterial and intravenous injection of 1 × 106 cells/kg of human cbMNC. Behavioral tests were performed before the MCAO, 1 week after MCAO, and at 3, 9, and 14 days after cbMNC injection. Brain infarct area and neurons in hippocampus were evaluated. Spontaneous activity was much significantly improved compared with the placebo group (p < 0.05). Comparing the neuron cells in hippocampus, intraarterial and intravenous have more changes in neurons morphology. No effect of cbMNC implantation in decreasing infarct area. Safety of xenogenic was confirmed by this study when the dosage of 1 × 106 cells/kg was used and showed their beneficial effects.
Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium Vista Budiariati; Dwi Budiono; Mokhamad Fahrudin; Berry Juliandi; Ratih Rinendyaputri; Arief Boediono
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.143 KB) | DOI: 10.4308/hjb.27.2.89

Abstract

Parthenogenesis is an artificial oocytes activation process without paternal contribution. Blastocyst, derived from parthenogenesis, is one of potential source for pluripotent stem cell propagation. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate. One of the component that predicted inhibit parthenogenetic embryo development is phosphate. Therefore, we try to modify culture medium in order to overcome that problem. The aim of this research was to produce and analyze the characteristics of parthenogenetic blastocyst developed in phosphate-free medium. Mouse oocytes obtained from adult female DDY by superovulation. The activator was strontium chloride 10 mM and diploidization with cytochalasin B 5 μg/ml. Medium for activation and culture medium were modified rat 1 cell embryo medium (MR1ECM) which is phosphate free. The results showed that parthenotes that were cultured in phosphate free medium reached higher blastocyst rate compared to the other groups. The increase of phosphate in culture medium lead to impaired parthenogenetic embryos development. Further experiment was made to analyze the differences between fertilized and parthenogenetic embryo in this medium. The experiment showed that diploid parthenogenetic could achieve high blastocyst rate (30.9±1.3%). The quality of diploid parthenogenetic blastocyst, based on cells number, viability, and ICM ratio, was lower than fertilized blastocyst.
Intra- and Interspecies Wing Venation Variations of Apis cerana and Apis nigrocincta Species in Indonesia Nisfia Rakhmatun Nisa; Berry Juliandi; Rika Raffiudin; Jauharlina Jauharlina; Mahardika Gama Pradana; Araz Meilin; Jasmi Jasmi; Yulia Pujiastuti; Puji Lestari; Fahri Fahri; Windra Priawandiputra; Tri Atmowidi
HAYATI Journal of Biosciences Vol. 29 No. 2 (2022): March 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.2.222-233

Abstract

Apis cerana has a wide distribution in Asia, including Sundaland, and is currently found in Wallacea, while the sister species, A. nigrocincta, is native in Sulawesi. The wide geographic distribution and the island isolation led to form morphological differences in the bees. The morph and wing venations are known to have a high genetic inheritance. Therefore, this research aimed to (1) analyze the landmark variation of wing venations of A. cerana from Sundaland and Wallacea, and A. nigrocincta from Sulawesi, (2) determine the relationship between these two bee species. The research was conducted by digitizing 550 wing venations based on nineteen landmarks. Our study on intraspecies showed that A. cerana Sumatra revealed a high variation in bending energy. Overall, the deformation grid of A. cerana from Sundaland has higher displacement than those from Wallacea, meaning higher variations of the Sundaland A. cerana. We found geometric morphometric markers of landmarks 16 and 17 in intraspecies and interspecies bees. Thus, these landmarks known as a cubital index can be used for species identification. The differentiation of interspecies has been shown in the PCA. Apis nigrocincta was separated from the single group of the centroid A. cerana and was supported by the Neighbor-Joining tree.
PCS-1 Development of Mouse Parthenogenetic Embryos in Phosphate Free Medium Vista Budiariati; Dwi Budiono; Mokhamad Fahrudin; Berry Juliandi; Ratih Rinendyaputri; Arief Boediono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (516.416 KB)

Abstract

Parthenogenesis is an artificial oocytes activation process without paternal contribution so that embryos will develop without fertilization [3]. The process of parthenogenesis as a reproductive strategy occurs in species of insect, pisces, or amphibian, which not require any implantation. Naturally, parthenogenesis is not common in mammals, but by understanding cellular mechanism during fertilization, it is possible to artificially activate mammalian oocytes.Blastocyst, derived from parthenogenesis, can be used for developmental study, embryo reconstruction, and one of potential source for pluripotent stem cells. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate.One of the component that has been predicted to inhibit parthenogenetic embryo development is phosphate. Haraguchi et al. (1996)    reported that phosphate caused a negative effect on in vitro culture of AKR/N mice fertilized embryos, removal of phosphate elements was significantly improved the blastocyst rate up to 42.6% [1]. The effects of phosphate also became an interesting finding in the study that reported mouse fertilized embryos could well developed in modified medium rat 1 cell embryo medium (MR1ECM) which not contained any phosphate [2].The effect of phosphate on in vitro culture of mouse parthenogenetic embryo has not been clear. The aim of this research was to analyze inhibitory effect caused by phosphate in the medium and compare the development pattern between parthenogenetic and fertilized embryos in order to reach optimal production of parthenogenetic blastocyst for further purposes.  
RAT BLOOD PROFILE EVALUATION AFTER Fe3O4/CHITOSAN COLLOID INJECTION Aloma Karo Karo; Irham Abdul Azis; Wildan Z.L.; Grace Tj. Sulungbudi; Berry Juliandi; Mujamilah Mujamilah
Jurnal Sains Materi Indonesia Vol 20, No 3: APRIL 2019
Publisher : Center for Science & Technology of Advanced Materials - National Nuclear Energy Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (807.897 KB) | DOI: 10.17146/jsmi.2019.20.3.5479

Abstract

RAT BLOOD PROFILE EVALUATION AFTER Fe3O4/CHITOSAN COLLOID INJECTION. The application of iron oxide (Fe3O4) magnetic nanoparticles in the biomedical field is still being explored, mainly related to its toxicity and side effects. This article reported results of the study aimed at analyzing the effect of chitosan-coated magnetic nanoparticles (NPM-C) on rat blood profiles. Magnetic colloid as much as 1 ml (concentration of 5 mg NPM-C / mL aquabidest) for 1 kG rat body weight was injected through intra-venous to the treated rat group (4 Wistar rats aged 6 months; weight ± 275 grams; male sex) while another four rats injected with sterile aquabidest used as a control group. The blood taking from each group of rats was carried out on 1 day before injection and several days after injection (days 1, 7, 14, 21, 28) through veins in the tail. To these blood samples, a series of blood profile analyzes is carried out including basic hematology, blood chemistry, and fragility of the erythrocyte membrane. The results of the analysis showed no significant differences between blood profiles after treatment and control, which indicated that chitosan-coated magnetic nanoparticles did not trigger cellular stress responses in the blood. The stability of blood magnetism analyzed by VSM (Vibrating Sample Magnetometer) also shows that magnetic nanoparticles are detected in the blood and tend to decrease in number with increasing time, so it is thought that these nanoparticles can be degraded or have been distributed into organs. These stable properties are analyzed due to an existence of chitosan coating around magnetic nanoparticles. Based on this study it can be concluded that up to the given concentration limit, iron oxide nanoparticles coated by chitosan are not toxic and have the potential to be used as drug carriers, MRI contrast agents, and other biomedical applications.
Mid-gestational exposure to histone deacetylase inhibitor suberoylanilide hydroxamic acid influence cortical interneuron and astrocyte in mouse brain Nunung Yuniarti; Berry Juliandi; Tsukasa Sanosaka; Kinichi Nakashima
Indonesian Journal of Biotechnology Vol 22, No 1 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (835.413 KB) | DOI: 10.22146/ijbiotech.25986

Abstract

Suberoylanilide hydroxamic acid (SAHA) has been reported preclinically to diffuse across the placenta and to be found in fetal plasma, suggesting that it can influence the fetus if taken by a pregnant cancer patient. In utero exposure of SAHA to mouse embryos during mid-gestation was found to perturb corticogenesis. However, the influence of in utero administration of SAHA to mouse embryos during mid-gestation on astrocyte, glial cell, and inhibitory neurons (interneurons) is yet to be reported. Pregnant dams were divided into control and SAHA groups and given methyl cellulose (as control) and SAHA orally once a day for 3 days during mid-gestation, starting from embryonic day (E)12 until E14. Astrocyte, interneuron, and behavior analyses were performed on the pups from postnatal day 7 until adulthood (3 months old). Brains were harvested and immunohistochemistry, Western Blot, and RT-PCR were performed on their cortex area. Transient exposure of SAHA to mouse embryos resulted in a decrease and increase in cortical astrocyte and interneuron, respectively. Meanwhile, adult SAHA mice displayed significantly increased anxiety, decreased memory, altered long-term cognitive functions, and reduced social interactions. Our study suggests that exposure to SAHA during prominent neurogenic periods might imbalance the normal excitatory:inhibitory neuron ratio required for the precise regulation of physiological functions in the brain.
Spatial learning and memory of young and aging rats following injection with human Wharton’s jelly‐mesenchymal stem cells Berry Juliandi; Wildan Mubarok; Dian Anggraini; Arief Boediono; Mawar Subangkit; Indra Bachtiar; Harry Murti; Kelvin Yaprianto; Boenjamin Setiawan
Indonesian Journal of Biotechnology Vol 26, No 2 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.64734

Abstract

Human Wharton’s jelly‐mesenchymal stem cells (hWJ‐MSC) are an emerging potential source of stem cells derived from the umbilical cord. Previous studies have shown their potential as treatment for traumatic brain injury and Parkinson’s disease. However, no study has yet investigated the effect of hWJ‐MSC injections in countering spatial learning and memory impairment in aging rats. The effect of hWJ‐MSC injection on young rats is also unknown. The objective of this research was to analyze the effect of an hWJ‐MSC injection on spatial learning, memory, density of putative neural progenitor cells (pNPC), and neuronal apoptosis in the dentate gyrus (DG) of young and aging rats. Injection of hWJ‐MSC did not change spatial learning and memory in young rats until two months post‐injection. This might be due to retained pNPC density and neuronal apoptosis in the DG of young rats after injection of hWJ‐MSC. In contrast, injection of hWJ‐MSC promoted both spatial learning and memory in aging rats, a finding that might be attributable to the increased pNPC density and attenuated neuronal apoptosis in DG of aging rats during the two months post‐injection. Our study suggests that a single injection of hWJ‐MSC might be sufficient to promote improvement in long‐term learning and memory in aging rats.
Kompleksitas Obyek dan Running-Wheel Mempengaruhi Novel Object Recognition Test pada Mencit (Mus musculus) NISFA HANIM; SALMAH WIDYASTUTI; ACHMAD ALFIYAN; MUHAMMAD AZHARI AKBAR; BERRY JULIANDI
Jurnal Sumberdaya Hayati Vol. 4 No. 1 (2018)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.4.1.7-11

Abstract

This research aimed to confirm the tendency of mice to novel object, effect of exercise (in running-wheel) toward memory of mice and to test tendency of mice in avoiding predator signal in novel object. Novel object recognition test (NORT) used to test the memory the day after acquisition phase (NORT I) and memory one week after exercise was given (running-wheel) (NORT II). The result showed that there was no tendency of mice in exploring toward novel object in both NORT I and NORT II. This might happen because the complexity of familiar object higher than novel object, so the familiar object could accommodate more activities. Exercise using running-wheel in mice had an effect on memory, it could be seen in decreasing duration of object exploration time from NORT I to NORT II. There was no tendency in avoiding predator’s signal on novel object which was attached by urine addition (odor signal).