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All Journal Journal of Biomedicine and Translational Research Mutiara Medika: Jurnal Kedokteran dan Kesehatan Biomedika KOVALEN: Jurnal Riset Kimia Medika Tadulako Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Media Medika Muda Healthy Tadulako Journal (Jurnal Kesehatan Tadulako)
David Pakaya
Departemen Histologi Fakultas Kedokteran Universitas Tadulako, Palu
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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EFEK AIR KELAPA (Cocos Nucifera Linn) DALAM MENCEGAH DEMAM Nayoan, Christin Rony; Fitriani, Junjun; Pakaya, David
Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan Vol 5, No 2 (2018)
Publisher : Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan

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Abstract

ABSTRAK Latar belakang: Tanaman kelapa (Cocos nucifera lin) memiliki banyak unsur-unsur yang bermanfaat untuk tubuh, dan air kelapa muda digunakan masyarakat untuk mengobati demam. Diperlukan uji untuk membuktikan bahwa air kelapa berkhasiat sebagai antipiretik. Tujuan: Untuk mengetahui efek antipiretik Air kelapa muda (Cocos nucifera lin), pada mencit yang diinduksi demam dengan vaksin DPT-Hb. Metode: Penelitian ini menggunakan rancangan acak lengkap, dengan sampel 25 ekor mencit jantan, berusia 8 minggu, berat badan (BB) 20–30 gr, yang dibagi menjadi 5 kelompok. K1: Kelompok Kontrol Negatif, K2: Kelompok Kontrol Positif (terapi paracetamol dosis 1,3 mg/20 grBB), K3: Kelompok Terapi Air Kelapa 0,5 cc, K4: Kelompok Terapi Air Kelapa 1 cc, K5: Kelompok Terapi Kelapa 1,5 cc. Mencit diinduksi vaksin DPT–Hb secara intraperitoneal, dilakukan pengukuran suhu rektal 2 jam dan 4 jam pasca induksi vaksin DPT–Hb. Analisis hasil penelitian menggunakan SPSS versi 22. Data yang didapat dilakukan uji Friedman. Hasil: Terdapat perbedaan suhu yang bermakna pada kedua waktu pengukuran suhu (p=0,003)dan terdapat penurunan suhu yang setara antara kelompok kontrol positif dengan kelompok perlakuan yang diterapi dengan air kelapa dosis 0,5 cc. Kesimpulan: Air kelapa (Cocos nucifera L.) mampu mencegah terjadinya demam setelah induksi DPT-Hb pada mencit. Kata Kunci: cocos nucifera L., antipiretik
PERANAN VITAMIN C PADA KULIT Pakaya, David
Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan Vol 1, No 2 (2014)
Publisher : Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan

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Abstract

Bagian Histologi, Fakultas Kedokteran dan Ilmu Kesehatan Universitas Tadulako
THE CORRELATION BETWEEN SEX AND AGE WITH THE HISTOPATHOLOGY OF LUNG CANCER PATIENTS IN Dr. SARDJITO HOSPITAL 2010 Pakaya, David; Maramis, Maynard Andrew; Setyawati, Tri
Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan Vol 4, No 2 (2017)
Publisher : Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan

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Abstract

Introduction: Lung cancer is the world's highest incidence and mortality. Sex and age are risk factors that affect the incidence of lung cancer besides smoking behavior. These risk factors associated with histopathologic which to base a diagnosis of lung cancer.Objective: To analyze the correlation of sex and age with histopathological of lung cancer patients who were treated at the Dr. Sardjito Hospital in 2010.Methods: The study was a retrospective cross-sectional design. Conducted at the Medical Records of Dr. Sardjito Hospital in February 2016. The samples were all lung cancer patients (total sampling) who were treated at the Dr. Sardjito Hospital from January 2010 - December 2010. The data was processed with SPSS software ver. 21.0 and analyzed with Chi square. Results: The male is that most lung cancer patients are 41 (62.1%). The age group most lung cancer patients in the age group > 40 years were 58 cases (87.9%). NSCLC is the most common histopathology of 60 people (90.9%). After analyzed with Chi square test the data showed p=0.810 for sex with histopathological correlation and p=0,095 for the age with histopathological correlation. Conclusions: There was no correlation between sex and age with histopathology of lung cancer patients who were treated at the Dr. Sardjito Hospital in 2010. Keywords: Sex, age, histopathology, lung cancer
GAMBARAN VARIASI GEN SEX HORMONE BINDING PROTEIN GLOBULIN (SHBG) MENGGUNAKAN PCR-RFLP Pakaya, David; Armadhari, Inna; Lestari, Ira Cinta
Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan Vol 4, No 3 (2017)
Publisher : Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan

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Abstract

Background: Sex hormone binding globulin (SHBG) are glycoprotein plasma that binding human sex steroid hormone. SHBG coded by gene at short arm 12-13 chromosome 17. SHBG variety are the product of mutation at exons 8 causing a single base substitution on codon 327 that coding the amino acid conversion and the addition of N-glycosylation group. Objective: To know the SHBG gene variation at the DNA from peripheral blood sample, using PCR-RFLP with BbsI restricted enzyme. Methods: DNA extraction from 5 peripheral blood sample and doing an amplification SHBG gene with PCR. The product are we do RFLP using restricted enzyme BbsI that visualized by electrophoresis at 3% agarose gel with EtBr. Result: 1 sample sliced at 290 bp and 4 sliced at 290 bp, 223 bp and 67 bp. Conclusion: There are found the genotip variation, homozygotes AA genotype and hetezygotes GA and homozygote AA genotype not founded. Key Word: SHBG Gene, variation, PCR-RFLP  Latar belakang: Sex hormone binding globulin (SHBG) merupakan glikoprotein plasma yang mengikat hormon steroid seks manusia. SHBG dikode oleh gen pada lengan pendek 12-13 kromosom 17. Adanya varian SHBG disebabkan mutasi titik pada ekson 8, menyebabkan substitusi basa tunggal pada kodon 327 (GACàAAC) yang mengkode perubahan asam amino (Asp327Asn)  serta menyebabkan penambahan gugus N-glikosilasi. Tujuan: Untuk mengetahui gambaran variasi gen SHBG pada DNA dari sampel darah tepi dengan menggunakan teknik PCR-RFLP menggunakan enzim BbsI. Metode: Dilakukan ekstraksi DNA dari 5 sampel darah tepi dan dilakukan amplifikasi gen SHBG dengan PCR, hasilnya dilakukan RFLP menggunakan enzim restriksi BbsI yang divisualisasikan dengan elektroforesis pada gel agarose 3% dengan EtBr.Hasil: Didapatkan 1 sampel terpotong pada 290 bp dan 4 sampel yang terpotong pada 290 bp, 223 bp dan 67 bp. Kesimpulan: Adanya variasi genotip homozigot AA dan heterozigot GA dan tidak ditemukan genotip homozigot GG. Kata Kunci: gen SHBG, variasi, PCR-RFLP
PERBANDINGAN DETEKSI PROLIFERASI SEL DENGAN IMUNOHISTOKIMIA PCNA DAN BrdU PADA PANKREAS TIKUS Pakaya, David; Setyowati, Wiwit; Susilowati, Rina
Biomedika Vol 12, No 2 (2020): Biomedika Agustus 2020
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v12i2.10641

Abstract

ABSTRAKPankreas merupakan jaringan labil karena terdiri dari sel-sel asinar yang selalu berproliferasi sepanjang hidupnya untuk menggantikan sel yang rusak. Proliferasi tersebut dapat diidentifikasi dengan pemeriksaan histologis, yaitu mikroteknik dengan pewarnaan imunohistokmia (IHC). Penelitian ini bertujuan untuk membandingkan deteksi proliferasi sel dengan IHC anti-PCNA dan anti-BrdU pada pankreas. Tikus diinjeksi BrdU secara intraperitoneal sebanyak 4 kali dengan selang waktu 3 jam dengan dosis BrdU 0,125 ml, 0,375 ml, 0,250 ml, dan 0,250 ml dan dibiarkan selama 1 malam. Jaringan pankreas dipotong sepanjang ± 5 mm dan direndam dalam fiksatif paraformaldehid 4%, kemudian dibuat blok paraffinnya. Blok parafin diiris dengan ketebalan 6µm untuk pengecatan hematoksilin eosin (HE) dan 4 µm untuk pengecatan IHC. Pengecatan IHC dengan antibodi anti PCNA (antibodi monoklonal PCNA 1;1000 dalam PBS). Pengecatan IHC dengan antibodi anti BrdU menggunakan antibodi primer (antibodi anti BrdU 1:1000). Pengecatan dianalisis secara kualitatif. IHC menggunakan antibodi anti-PCNA dan anti-BrdU menunjukkan adanya penyebaran warna coklat gelap yang menandakan proliferasi sel aktif, dimana sel yang terekspresi pada BrdU lebih sedikit dibandingkan PCNA.  Penelitian ini menyimpulkan bahwa proliferasi sel pankreas dapat terekspresi melalui perwarnaan IHC dengan antibodi anti PCNA dan anti BrdU.Kata kunci: Imunohistokimia, PCNA, Brdu, Proliferasi ABSTRACT                   The pancreas is a labile tissue because it consists of acinar cells which proliferate throughout their lives to replace damaged cells. The proliferation can be identified by histological examination, that is microtechnics with immunohistochmia (IHC) staining. This study aims to compare the detection of cell proliferation with anti-PCNA and anti-BrdU IHC in the pancreas. The rats were injected BrdU intraperitoneally 4 times with an interval of 3 hours with a dose of BrdU 0.125 ml, 0.375 ml, 0.250 ml, and 0.250 ml and left for 1 night. Pancreatic tissue were necropsy along ± 5 mm and soaked in 4% paraformaldehyde fixative, then made paraffin block. The paraffin block was sliced to a thickness of 6 µm for hematoxylin eosin (HE) and 4 μm for IHC painting. IHC used anti PCNA antibodies (PCNA monoclonal antibody 1; 1000 in PBS) and anti BrdU antibodies (anti BrdU primary antibodies 1: 1000). Staining was analyzed qualitatively. IHC with anti-PCNA and anti-BrdU antibodies showed the spread of dark brown which indicates active cell proliferation, whereas cells expressed in BrdU are less than PCNA. This study concluded that pancreatic cell proliferation can be expressed through the coloring of IHC with anti-PCNA and anti-BrdU antibodies.Keyword: Immunohistochemistry, PCNA, Brdu, Proliferation
PEMANFAATAN HEWAN COBA PADA PENELITIAN NEURODEGERATIF Pakaya, David; Susilowati, Rina
Healthy Tadulako Journal (Jurnal Kesehatan Tadulako) Vol. 6 No. 2 (2020)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.682 KB) | DOI: 10.22487/htj.v6i2.91

Abstract

Penyakit neurodegeneratif berhubungan dengan kerusakan bahkan kematian sel-sel saraf progresif. Untuk mempelajarinya diperlukan suatu kondisi serupa dari penyakit manusia pada hewan coba. Famili muridae merupakan hewan coba yang paling sering digunakan. Penelitian ini bertujuan untuk Mengetahui berbagai metode pengkondisian hewan coba telah dikembangkan dan digunakan dalam penelitian penyakit neurodegenratif. Dilakukan penelusuran literatur secara on-line dengan menggunakan search engine PubMed dengan kata kunci "animal model" AND "neurodegenerative". Berbagai metode pengkondisian hewan coba tersebut dapat dilakukan dengan cara merekayasa genetika, model cedera ataupun induksi bahan kimiawi dengan berbagai metode.
ANALISIS SITUASI PENANGGULANGAN TUBERKULOSIS PARU DI KABUPATEN SIGI Suarayasa, Ketut; Pakaya, David; Felandina, Yeni
Healthy Tadulako Journal (Jurnal Kesehatan Tadulako) Vol. 5 No. 1 (2019)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.704 KB) | DOI: 10.22487/htj.v5i1.106

Abstract

Penyakit tuberkulosis (TB) masih merupakan masalah kesehatan yang membebani negara-negara berkembang. Pemerintah terus berupaya melalukan penyelesaian masalah TB di Indonesia melalui Rencana Aksi Nasional Penanggulangan TB tahun 2016 – 2019, sebagai langkah konkrit penanggulangan TB secara komprehensif di seluruh Indonesia termasuk di kabupaten Sigi. Sehingga dalam pelaksanaannya harus dilakukan evaluasi. Setelah setahun pelaksanaan renana aksi Nasional tersebut angka penemuan kasus TB di kabupaten Sigi masih rendah. Banyak faktor yang mempengaruhi hal tersebut, seperti pelaporan kasus yang belum maksimal, mutu laboratorium diagnosis yang belum baik maupun belum terlaksananya pengobatan pencegahan yang baik pada kelompok berisiko. Akan tetapi angka keberhasilan pengobatan justru berada diatas rata-rata Nasional.
Nanotechnology in Metformin Delivery: Fasting Blood Glucose and Neutrophil-Lymphocyte Ratio of Diabetic Rat Model David Pakaya; Laurents Christovel Iban Demen; Haerani Harun; Sarifuddin Anwar; Gabriella Bamba Ratih Lintin
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 22, No 1 (2022): January
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v22i1.13358

Abstract

Diabetes Mellitus (DM) is a chronic inflammatory disease characterized by hyperglycemia and increasing the neutrophil-lymphocyte ratio (NLR). Metformin has been widely used to treat hyperglycemia. Metformin nanoparticles can improve bioavailability and may reduce inflammation. This study aims to analyze the effectiveness of metformin nanoparticles delivery through fasting blood glucose (FGB) level and NLR in the diabetic rat model. This study used 16 white male Wistar rats, 8 weeks of age, and body weight (BW) 250-350 grams. The streptozotocin (STZ) 40 mg/kg BW were injected i.p. Rats were divided into 4 groups; K1: normal control; K2: negative control (diabetes model); K3: diabetes model treated with metformin 100mg/Kg BW; K4: diabetes model treated with nanoparticle metformin 100mg/kg BW. Blood analysis tests were conducted using the Pentra hematology analyzer. Data were analyzed using the Graphpad Prism program with the nonparametric Kruskal Wallis test. The K3 group showed a periodical decrease in FBG level from day 7 to day 28 by 122 ± 11.31 mg/dL, and the mean NLR value was 0.48 ± 0.3 x 103/uL. Group of K4 periodically decreased in FBG level, indicating that it was closer to normal than K3. The result showed that at day 28.79 ± 15.39 mg/dL, the mean NLR value slightly increased compared to the K3 group by 0.54 ± 0.3 x103/uL. The statistical tests showed a significant difference between the level of FBG (p 0.0089) but no significant difference in NLR (p 0.347). Metformin nanoparticles could decrease FBG levels and effectively reduce the NLR in the diabetic rat model.
PERBANDINGAN DETEKSI PROLIFERASI SEL DENGAN IMUNOHISTOKIMIA PCNA DAN BrdU PADA PANKREAS TIKUS David Pakaya; Wiwit Setyowati; Rina Susilowati
Biomedika Vol 12, No 2 (2020): Biomedika Agustus 2020
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v12i2.10641

Abstract

ABSTRAKPankreas merupakan jaringan labil karena terdiri dari sel-sel asinar yang selalu berproliferasi sepanjang hidupnya untuk menggantikan sel yang rusak. Proliferasi tersebut dapat diidentifikasi dengan pemeriksaan histologis, yaitu mikroteknik dengan pewarnaan imunohistokmia (IHC). Penelitian ini bertujuan untuk membandingkan deteksi proliferasi sel dengan IHC anti-PCNA dan anti-BrdU pada pankreas. Tikus diinjeksi BrdU secara intraperitoneal sebanyak 4 kali dengan selang waktu 3 jam dengan dosis BrdU 0,125 ml, 0,375 ml, 0,250 ml, dan 0,250 ml dan dibiarkan selama 1 malam. Jaringan pankreas dipotong sepanjang ± 5 mm dan direndam dalam fiksatif paraformaldehid 4%, kemudian dibuat blok paraffinnya. Blok parafin diiris dengan ketebalan 6µm untuk pengecatan hematoksilin eosin (HE) dan 4 µm untuk pengecatan IHC. Pengecatan IHC dengan antibodi anti PCNA (antibodi monoklonal PCNA 1;1000 dalam PBS). Pengecatan IHC dengan antibodi anti BrdU menggunakan antibodi primer (antibodi anti BrdU 1:1000). Pengecatan dianalisis secara kualitatif. IHC menggunakan antibodi anti-PCNA dan anti-BrdU menunjukkan adanya penyebaran warna coklat gelap yang menandakan proliferasi sel aktif, dimana sel yang terekspresi pada BrdU lebih sedikit dibandingkan PCNA.  Penelitian ini menyimpulkan bahwa proliferasi sel pankreas dapat terekspresi melalui perwarnaan IHC dengan antibodi anti PCNA dan anti BrdU.Kata kunci: Imunohistokimia, PCNA, Brdu, Proliferasi ABSTRACT                   The pancreas is a labile tissue because it consists of acinar cells which proliferate throughout their lives to replace damaged cells. The proliferation can be identified by histological examination, that is microtechnics with immunohistochmia (IHC) staining. This study aims to compare the detection of cell proliferation with anti-PCNA and anti-BrdU IHC in the pancreas. The rats were injected BrdU intraperitoneally 4 times with an interval of 3 hours with a dose of BrdU 0.125 ml, 0.375 ml, 0.250 ml, and 0.250 ml and left for 1 night. Pancreatic tissue were necropsy along ± 5 mm and soaked in 4% paraformaldehyde fixative, then made paraffin block. The paraffin block was sliced to a thickness of 6 µm for hematoxylin eosin (HE) and 4 μm for IHC painting. IHC used anti PCNA antibodies (PCNA monoclonal antibody 1; 1000 in PBS) and anti BrdU antibodies (anti BrdU primary antibodies 1: 1000). Staining was analyzed qualitatively. IHC with anti-PCNA and anti-BrdU antibodies showed the spread of dark brown which indicates active cell proliferation, whereas cells expressed in BrdU are less than PCNA. This study concluded that pancreatic cell proliferation can be expressed through the coloring of IHC with anti-PCNA and anti-BrdU antibodies.Keyword: Immunohistochemistry, PCNA, Brdu, Proliferation
Aktivitas Antischistosomiasis Sediaan Nanopartikel Ekstrak Biji Pinang pada Tikus Putih Jantan Terinfeksi Schistosoma japonicum: The Antischistosomiasis Activity Test on Nanoparticles Ethanolic Extract of Betel Nut to Male Rats Infected with Schistosoma japonicum Marzela Dewi; David Pakaya; Joni Tandi
KOVALEN: Jurnal Riset Kimia Vol. 7 No. 1 (2021): Edisi April
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2021.v7.i1.15438

Abstract

Schistosomiasis is a disease caused by Schistosoma japonicum worms and Oncomelania hupensis lindoensis as the intermediate snails. Praziquantel is a drug used to reduce the prevalence of schistosomiasis. However, its use has several side effects such as headaches, dizziness, nausea, vomiting as well as abdominal, joint, and muscle pain. The development of alternative drugs is a solution to reduce side effects. Betel (A. catechu) seeds are known to the public to have an anthelmintic effect because they contain alkaloids, tannins, flavan, phenolic compounds, as well as arecoline. To increase the bioavailability and efficacy of the extract, the seeds were prepared in the formulation of nanoparticles. This study aims to determine the antischistosomiasis activity of the ethanol extract nanoparticles of betel nut as an antischistosomiasis. The rats were divided into 9 treatment groups consisting of normal, negative and positive control groups, extract treatment and treatment of nanoparticle preparations with dose variations of 30, 60, and 120 mg/kgBW. The parameter observed was the number of S. japonicum eggs in rat feces before and after treatment. Data obtained from the mean number of S. japonicum eggs was tested for normality with Saphiro-Wilk test, and showed that the data were not normally distributed. Furthermore, nonparametric statistical analysis was carried out with Kruskal-Wallis which showed there was no significant difference in the mean number of S. japonicum eggs in all treatment groups. The results showed that the ethanol extract nanoparticles of A. catechu had antischistosomiasis activity.
Co-Authors Anak Agung Istri Sri Wiadnyani Arlita Leniseptaria Antari Armadhari, Inna Aryoko Widodo Christin Rony Nayoan Eka Mutiara Herman Elfiana Ibrahim Felandina, Yeni Fikhta Agnesya Tarusu Fitriani, Junjun Gabriella Bamba Ratih Lintin Haerani harun Imtihanah Amri Indah Saraswati Iniche Tinta Ira Cinta Lestari Joni Tandi, Joni Ketut Suarayasa Laurents Christovel Iban Demen Maramis, Maynard Andrew Marzela Dewi Marzela Dewi Niluh Puspita Dewi Puput Octaviani Solehah Qosimah, Dahliatul Rina Susilowati Sarifuddin Anwar Tri Setyawati Wiwit Setyowati Yuli Fitriana