Feliatra, Feliatra
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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DEGRADATION ABILITY AND IDENTIFICATION OF OIL DEGRADATION BACTERIA BY 16S rRNA SEQUENCING Shaumi, Nurul; Nursyirwani, Nursyirwani; Feliatra, Feliatra
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTPollution in the marine environment caused by industrial activity, marine transportation, offshore mining and oil refineries, one of which comes from petroleum contamination. One of  efforts to prevent and to manage oil pollutionwas by the use of oil degrading bacteria. The objective of this research was to examine degradation ability of marine bacteria isolates and identify the bacterial isolate based on 16S rRNA sequence. The research was conducted from March to May 2018. The oil degradation analysis was conducted at Laboratory of Marine Microbiology,Faculty of Fishery and Marine Science, while the molecularidentification of bacteria was done in Genetics Laboratory, Faculty of Mathematics and Natural Sciences, University of Riau. The results showed that five isolates of oil degradation bacteria, selected(BM 1d, BM 7b, BM 7c, BM 14a and BM 14b) were able to degrade crude oil at different concentrations. The highest degradation at 1% concentration is indicated by BM 14b isolate, at 2% concentration was indicatedby BM 1d isolate and at 3% concentration was indicated BM 14b isolate. From the degradation analysis results, three isolates (BM 1d, BM 14a, BM 14b) have the highest degradation ability to determine the type of oil degrading bacteria. The molecular identification results by the 16S rRNA sequencing and BLAST analysis showed that BM 1d isolate were similar to Bacillus sp. (95%), BM 14a isolate were similar to Bacillus flexus (92%) and BM 14b isolate were similar to Bacillus cereus (98%). Keyword : Oil Degradation Bacteria , Crude Oil, 16S rRNA, Bacillus
DENSITY OF Pseudomonas sp. BACTERIA AND HETEROTROPHIC BACTERIA IN DUMAI SEAWATER OFRIAU PROVINCE Masdini, Alvira; Feliatra, Feliatra; Effendi, Irwan
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT Pseudomonas sp. is a hydrocarbonoclasticbacteria that is capable of degrading various types of hydrocarbons. Pseudomonas sp.including bacteria that have the potential to become pathogens. This research was conducted in April to May 2018. This study aims to calculate the density of Pseudomonas sp.bacteriaand heterotrophic bacteria in Dumai sea waters, comparison of the desity of Pseudomonas sp. bacteria to heterotrophic bacteria at each station and the resistance of Pseudomonas sp. to antibiotics. The result showed that densityPseudomonas sp. ranged from 2.25 x 105- 6.08 x 105 CFU/ml. The highest number of Pseudomonas sp.at station 1 and lowest at station 5. The total number of heterotrophic bacteria ranged from 7.37 x 106 - 10.38 x 106 CFU/ml. The highest number of heterotrophic bacteria at station 4 and the lowest at station 5.The highest percentage rasioof density of Pseudomonas sp. bacteri to heterotrophic bacteria was in station 1 (6.26%) and the lowest was in station 4 (2.86%). The results of isolation of bacteria obtained 25 pure isolates and tested for bacterial resistance to antobiotics. Results of isolation bacteria obtained 25 pure isolates and tested the resistance ofPseudomonassp. bacteriato antibiotics.Chloramphenicolantibiotic test results showed 2 isolates classified as sensitive with inhibition zone of 21,3 – 21,9 mm, 1 isolate including resistance with 9,7 mm inhibition zone, with a 22 isolates classified as intermediate with a inhibition zone of 9.7 – 19.6 mm. Two other antibiotics Penicillin and Isoniazid, all bacterial isolates were resistant with the inhibition zone range from 1.2 – 4.6 mm and 1 – 2.8 mm respectively Keyword: Density, Pseuomonas sp.,Heterotrophic Bacteria, Antibiotics 
MICROSCOPIC ANALYSIS THE GROWTH OF Azolla microphylla IN BRACKISH WATER Daulay, Anita Diah Permata Sari; Effendi, Irwan; Feliatra, Feliatra
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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                                  AbtrakAzolla microphyllais a water fern that floats and rich of protein, essential amino acids, vitamins and minerals. The ferncan be found in ponds, ditches,warm temperature fields and tropical regions throughout the world. Research on microscopic analysis the growth of Azolla microphylla in brackish water was carried out from April to June 2018. A. microphylla was grown in containers with a volume capacity of 20 liters. The container was filled with planting media, then filled with brackish water to a volume of 10-15 liters and this ecosystem is set to 0 ‰, 1 ‰, 2 ‰ and 3 ‰. The growth of A. microphylla was observed by measuring biomass growth, colony growth, and double time growth, microscopic observations of leaves, rhizomes and rootsA. microphylla. From the results of the study it was found that A. microphylla can grew in brackish water (salinity 1‰, 2‰ and 3‰), however growth tends to be slower than in fresh water (salinity 0 ‰), and also can be seen based on the cross section of leaves, roots and rhizomes that grew optimally at salinity 0 ‰, and experience changes in shape and color in the cross section and do not grew optimally at salinity 1‰, 2‰ and 3‰.Keywords: Azolla microphylla growth, microscopic analysis, brackish water
Secondary Metabolite Test of Heterotrophic Bacteria from Sea Water in Sungai Kayu Ara Village Against Pathogenic Bacteria Y Hutasoit, Anjeli; Feliatra, Feliatra; Dahliaty, Andy
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT This research was conducted from April - July 2018 at the Laboratory of Organic Chemistry and Natural Materials Synthesis Research, Faculty of Mathematics and Natural Sciences and Marine Microbiology Laboratory in the Department of Marine Sciences, Faculty of Fisheries and Marine Sciences, University of Riau. The purpose of this research is to know the ability of secondary metabolites to inhibit heterotrophic bacteria in inhibiting the activity of pathogenic bacteria (Vibrioalginolyticus, Aeromonas hydrophila and Pseudomonas aeruginosa). The research method used is the experimental method. 10 isolates of heterotrophic bacteria were obtained from a collection of Marine Microbiology, Faculty of Fisheries and Marine laboratories which were able to produce secondary metabolites. Each bacterial isolate was tested on pathogenic bacteria, namely Aeromonashydrophila (P), Pseudomonas aeruginosa (Q), Vibrio alginolyticus (R) using paper discs and each isolate was carried out 3 repetitions, so that 90 experiments were obtained. Test results of secondary metabolites of 10 heterotrophic bacterial isolates have the ability to inhibit the growth of pathogenic bacteria. The best isolate is J isolates with clear zones formed wider than the other test bacteria which is 12,35 mm and the activity of the bacteria was 1877,78 mm2 / ml. This inhibitory power is caused by the presence of compounds that are anti-microbial namely secondary metabolites. Isolates A, D, F, G, H are resistant to pathogenic bacteria Aeromonas hydrophilla, isolates D, E, G, J are resistant to pathogenic bacteria Pseudomonas aeruginosa. This is indicated by the presence of a cloudy zone around the disc paper that is formed during the inhibitory test Keyword : Secondary Metabolites, Heterotrophic Bacteria, Inhibitory Power
THE POTENTIAL OF THE ISOLATED PROBIOTICS BACTERIAL FROM GIANT PRAWNS’ DIGESTIVE TRACT (Macrobrachium Rosenbergii, De Man) WITH 16S DNA SEQUENCES TECHNIQUE Feliatra, Feliatra; Yoswaty, Dessy; Lukystyowati, Isye; Hasyimi, Wahid
Aquacultura Indonesiana Vol 15, No 2 (2014): Volume 15 Issue 2 Year 2014
Publisher : Indonesian Aquaculture Society (MAI)

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Probiotics is a bacterial product that has beneficial for fish; both for growth and body's defenses from bacteria desease. The aim of the study is to determine the types of candidate of probiotic bacteria from the digestive tract of giant prawns that is found in Teratak Buluh Pekanbaru, Riau. This research was conducted at the Laboratory of Microbiology and Biotechnology of Fishery Faculty of Fisheries and Marine Sciences University of Riau, Pekanbaru to get a candidate of Probiotic Bacteria and do molecular examination that consists of purification of electrophoresis gel and DNA sequencing at the Institute for Biotechnology Serpong, Tangerang Banten. The method of this study is a survey method that used physical and biochemical test and molecular identification . The results showed that eleven isolates of bacterial are the species Bacillus BDU4 strain from A Bacteria isolates with 99 %  homology and 10 isolates of  bacterial are bacillus genus because the degree of homology below 97% and they are considered as the original indonesia’s new species probiotic bacteria.