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Khusniati, Tatik
Research Center for Biology-Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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KUALITAS NUTRISI ANEKA TEPUNG DAN KUE TALAM BERBASIS BAHAN PANGAN PULAU ENGGANO DENGAN PENAMBAHAN Lactobacillus plantarum B110 [Nutrition Quality of Various Flour and Talam Cake Based on Enggano Island Food Material Added with Lactobacillus plantarum B110] Khusniati, Tatik; Sulistiani, Sulistiani; Choliq, Abdul; Nanta, Dhea Loka; Wardani, Dita Kusuma; Saraswati, Dahniar
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i3.2222

Abstract

Nine foodstuffs from Enggano island were processed to flour as wheat flour alternative. To increase flour quality and its derivative product, Lactobacillus-plantarum B110 was added those flour and talam cake made from such flour. They were forest cassava (Dioscorea sp.), ararut sago (Marantha arundinacea), tacca (Tacca Leontopetaloides), egg taro (Alocasia sp.), oil taro (Alocasia sp.), gadung (Dioscorea hispida), gogo rice (Oryza sativa), corn (Zea mays), and belinjo (Gnetum gnemon).  The quality assessment consisted of HCN detection (qualitative), nutrition contents (proximate analysis), acid  (titration method), glucose (GOD-POD kit), and organoleptic tests (20 panelists). The results show that acid and glucose contents of the nine flours increased after L. plantarum B110 addition. Acid content of  the flours was 0.0144-0.2475%, while  glucose was  0.056-0.449%.  Carbohydrate, energy and acid contents of L. plantarum B110 talam cake were higher than control, which those were 34.13%, 190.33 cal/100gr, 0.00082%, while protein, lipid, water, ash were 1.75%, 5.22%, 57.9 %, 1.01%, respectively. The talam cake was accepted by panelists with values: 5.50 (taste), 5.6 (colour), 4.55 (flavour), 4.00 (texture), 4.35 (homogeneous). It was concluded that the flour and talam cake quality increased with addition of L. plantarum B110. 
POTENSI ANTIBAKTERI TIGA SPESIES BAKTERI ASAM LAKTAT ASLI ENGGANO TERHADAP BAKTERI PATOGEN DAN PEMBUSUK MAKANAN [Antibacterial Potential of Three Indigenous Lactic Acid Bacteria Species from Enggano Against Pathogenic and Food Spoilage Bacteria] sulistiani, sulistiani; Khusniati, Tatik
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2879.838 KB) | DOI: 10.14203/beritabiologi.v15i3.2410

Abstract

Lactic acid bacteria (LAB) strains have been known for their antimicrobial compounds that are important for food industry,including extension of expired time for food products. In this study, LAB isolates were isolated anaerobically from coconut sap using MRSA medium. Antibacterial analysis was carried out using microdilution method on microplate. The antibacterial assay showed that 85 isolates of LAB exhibit antibacterial activities against Escherichia coli, Salmonella enterica, Staphylococcus aureus, Pseudomonas aeruginosa, Listeria monocytogenes, and Bacillus cereus. Molecular identification of 13 physiologically characterized LAB strains based on 16S rDNA sequence determined three species, namely, Leuconostoc mesenteroides (strain EN17-1, EN17-8, EN17-12, EN17-15, EN17-34, EN17-41, EN17-43, EN17-45, EN17-46, EN38-34), Lactobacillus fermentum (strain EN17-2, EN38-44) and L. satsumensis strain EN38-32. The strong and wide broad spectrum antibacterial activity was produced by L. satsumensis strain EN38-32. 
Co-Authors Abdul Choliq Dita Kusuma Wardani Nanta, Dhea Loka Saraswati, Dahniar Sulistiani Sulistiani, Sulistiani