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Imelda, Maria
Research Center for Biology-Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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ANALISA KESTABILAN GENETIK PISANG KEPOK ‘UNTI SAYANG’ HASIL MIKROPROGASI DENGAN MARKA RAPD DAN ISSR [Genetic Stability Analyses of Micropropagated Pisang Kepok ‘Unti Sayang’ by RAPD and ISSR Markers] Poerba, Yuyu Suryasari; Imelda, Maria; Martanti, Diyah
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.276 KB) | DOI: 10.14203/beritabiologi.v11i2.497

Abstract

Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeats (ISSR) markers were used to evaluate the genetic stability of micropropagated plants of ‘Pisang Kepok Unti Sayang’ at various stage of in vitro sub-cultures and in vivo plant material.All RAPD and ISSR profiles from micropropagated plants were monomorphic and similar to those of field grown control plants until stage tenth of sub cultures (V1S10). No variation was detected within the micropropagated plants, except for C12 (V1S44),G7 (V1S48 ), I11 and I12 (V1S10).RAPD and ISSR marker were both could be used to test the genetic stability of micropropagated bananas using the developed protocol.
MIKROPROPAGASI TANAMAN ILES-ILES {Amorphophallus muelleri Blume) Imelda, Maria; Wulansari, Aida; Poerba, Yuyu S
BERITA BIOLOGI Vol 8, No 4 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (907.57 KB) | DOI: 10.14203/beritabiologi.v8i4.2117

Abstract

In Indonesia, iles-iles (Amorphophallus muelleri Blume) has not yet been cultivated intensively; their cultivation area is still limited. This species contains high glucomannan, which is useful as food diet, paper pulp, textile, paint, film-negative, celluloid and cosmetic industry. The cultivation of A. muelleri is hampered by limited genetic quality of plant.The species is triploid (2n=3x=39), the seed is developed apomictically, and pollen production is low.This may explain that the species is difficult to breed conventionally and genetic variability in the existing landraces cultivars is rather limited. Genetic variability of this plant is therefore can be achieve by induced mutation through tissue cultures for use in breeding program to develop better cultivars.Developing an efficient and effective micropropagation of the species is therefore important for use in the genetic improvement program.In other hands, the prospect for development and export of iles-iles is high since the demand from Japan alone has not been fulfilled. Propagation of iles-iles is generally done by splitting tubers, bulbils or leaf cuttings, but this method can not yield planting materials in large quantities within a relatively short time. In this research, young shoots which had just appeared from tubers were used as a source of explants. Sterilization of the explants was carried out in 0.05 % HgCl, solution for 20 min, rinsed several times with sterile distilled water and then cultured on Murashige and Skoog (MS) medium containing 0.1-0.2 mg/1 Thidiazuron (TDZ), 0.5-1.0 mg/1 Benzylaminopurine (BAP) and 0.5-1.0 mg/1 Kinetin (KIN) singly or in combination. Acclimatization of plantlets was done on 3 kinds of media namely (A), soil + compost, (B) soil + compost.+ cocopeat, and (C) soil + cocopeat. The results showed that the best medium is MS containing 0.2 mg/1 TDZ and 0,5 mg/1 BAP for in vitro shootbuds induction and proliferation of iles-iles, while MS without plant growth regulators is suitable for shoot growth and root formation and soil + compost + cocopeat for acclimatization of plantlets.
PERBANYAKAN IN VITRO KELADI TIKUS (Typhonium flagelliforme (Lodd) BI.), TANAMANA YANG BERPOTENSI SEBAGAI OBAT KANKER Imelda, Maria
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3676.286 KB) | DOI: 10.14203/beritabiologi.v6i4.3452

Abstract

Keladi tikus (Typhonium flagelliforme (Lodd) BI.) of the Araceae generally grows wild in the open or in slightly shaded areas. The active compounds of the plant have not yet been known, however its potential for cancer medication has been reported by several media. Since the plant is being hevaly removed in their habitat, research for adoption of in vitro technique for production of their planting materilals is needed. Lateral shoots of a 2-cm tuber, were sterilized with 30% sodium hypochlorite for 15 minutes. After several rinses with sterile distilled water, the shoots were cultured on a soild Murashige & Skoog (MS) medium supplemented with BAP (0,25;0,5;0,75;1,0 mg/l) in combination with IBA (0,25;0,5;0,75;1,0 mg/l). All cultures were incubated at 26 C under fluorestcent lights of 16 hours/day. Rooting of shoots was induced on MS medium without growth regulators and the plantets were established in a potting medium of sand,compost and soil (1:1:1). The result showed that the highest rate of shoot proliferation and growth was on MS medium supplemented with 0,5 mg/l 1 BAP and 0.25 mg/l IBA. Using this medium,  a sixteen-fold increase in shoot multiplication can be achieved with survival rate of 95%.
Co-Authors Diyah Martanti, Diyah Wulansari, Aida Yuyu S Poerba Yuyu Suryasari Poerba