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Norma Tiku Kambuno
Poltekkes Kemenkes Kupang
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ANALISIS CEMARAN LOGAM TIMBAL (PB) DAN TEMBAGA (CU) DALAM TEPUNG TERIGU DENGAN METODE SPEKTROFOTOMETRI SERAPAN ATOM LOGA, MARIA CARITAS N.A.I; KAMBUNO, NORMA TIKU
JURNAL INFO KESEHATAN Vol 12 No 1 (2014): JURNAL INFO KESEHATAN
Publisher : Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.88 KB) | DOI: 10.31965/infokes.v12i1.43

Abstract

National Standardization Agency of Indonesia in the SNI No. 01-3751-2006 on wheat flour as food ingredients include Lead (Pb) and Copper (Cu) as metal contaminants. Consumption of food or beverages that contain heavy metal contamination on - constantly, will lead the process of bioaccumulation of metals. The impactsare a disorder of the nervous system, stunted growth, reproductive disorders, paralysis and premature death, and may also reduce the level of intelligence of children. The purpose of this study was to determine the metal content of Lead (Pb) and Copper (Cu) in wheat flour brand Gatotkaca and compare with SNI requirements. This study uses a dry destruction by Atomic Absorption Spectrophotometry. The analysis showed that wheat flour brand Gatotkaca in the Oeba market Kupang containing lead (Pb) metallic of 2.2983 mg/kg while the metal content of copper (Cu) of 2.7813 mg / kg. It was concluded that the metal contamination levels of lead (Pb) are not match while the metal concentrations of copper (Cu) are match with SNI.
Identifikasi Bakteri Gram Negatif Galur Extended Spectrum Beta Lactamase Pada Ruang NICU RSUD Prof. DR. W. Z. Johannes Kupang Kambuno, Norma Tiku; Fanggidae, Dicky
JURNAL INFO KESEHATAN Vol 15 No 2 (2017): JURNAL INFO KESEHATAN
Publisher : Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (811.387 KB)

Abstract

Extended Spectrum Beta Lactamase (ESBL) adalah enzim yang mampu menghidrolisis antibiotik dari golongan penicillin, cephalosporin generasi I, II, III dan monobactam. ESBL paling banyak diisolasi dari Enterobacteriaceae khususnya Escherichia coli dan Klebsiella pneumoniae. Penyebaran Enterobacteriaceae penghasil ESBL juga dapat terjadi karena adanya mutasi. Kasus deteksi ESBL pada rumah sakit telah banyak dilaporkan di seluruh dunia termasuk Indonesia. Tujuan penelitian ini adalah untuk mengidentifikasi Enterobacteriaceae yang termasuk galur ESBL yang diisolasi dari ruangan NICU RSUD Prof. Dr. W. Z. Johannes Kupang tahun 2015. Penelitian ini adalah penelitian deskriptif dengan pendekatan cross sectional. Sampel yang digunakan adalah 18 spesimen swab dari fasilitas ruangan dikumpulkan dengan metode accidental sampling. Spesimen swab ditanam pada Blood Agar Plate dan Mac Conkey Agar. Metode identifikasi bakteri dilengkapi dengan uji mikroskopis, dan uji biokimia. Klebsiella sp berhasil diidentifikasi kemudian dilanjutkan dengan uji kepekaan antimikroba (Kirby Bauer) terhadap ceftazidime dan ceftriazone. Uji konfirmasi ESBL menggunakan metode Double Disc Sinergy Test (DDST). Hasil uji kepekaan antibiotik menunjukan Klebsialla sp. resisten terhadap antibiotik ceftazidime dan ceftriazone. Uji DDST menunjukkan produksi ESBL dari Klebsiella sp. Disimpulkan bahwa ditemukan Enterobacteriaceae penghasil ESBL yakni Klebsiella sp yang sudah menunjukkan resistensi pada cephalosporin generasi ketiga (Ceftazidime dan Ceftriazone).
Total Lymfosit Count (TLC) with CD4 in HIV/AIDS Patients at Kupang Wuan, Adrianus Ola; Herlinalt Gloria Banunu, Ayorince; Kambuno, Norma Tiku
Jurnal Teknologi Laboratorium Vol 8 No 2 (2019): 2019 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.602 KB) | DOI: 10.29238/teknolabjournal.v8i2.189

Abstract

Human Immunodeficiency Virus (HIV) is a retrovirus originating from the retroviridae family of the genus lentivirus that infects and damages cells that have a molecule Cluster of Differentiation 4 (CD4), especially T lymphocytes that have receptors with high affinity for HIV. Total lymphocyte count / TLC has been proposed as an alternative guide to CD4 in limited health facilities. This study aims to determine the correlation between Total Lymphocyte Count (TLC) and CD4 in HIV/AIDS patients in the W.Z. Johannes Kupang hospital. The type of this research was observational analytic with a cross-sectional design. The study was conducted on 121 samples of patients who performed CD4 examination and Total Lymphocytic Count (TLC) in the laboratory of W.Z.Johannes Kupang Hospital. The Spearman correlation test shows a significance value of 0,000 with a Spearman correlation value of 0.799. Based on the results of this study it can be concluded that there is a significant correlation between Total Lymphocyte Count and CD4 and shows the direction of positive correlation with a very strong relationship, where the increase in the number of Total Lymphocyte Count is in line with the increase in CD4 counts.
The potential of ethanol extract of white pomegranate leaves (Punica granatum L) as anti-bacterial Susilawati, Ni Made; Arnawa, I Gede Putu; Octrisdey, Karol; Kambuno, Norma Tiku
Jurnal Teknologi Laboratorium Vol 9 No 2 (2020): 2020 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i2.258

Abstract

Treatment of infections using penicillin-derived antibiotics such as methicillin has been found to cause antibiotic-resistant bacteria. This bacteria could produce a beta-lactamase enzyme to form a resistant strain. Research on antibacterial activity continues to develop. Pomegranate (Punica granatum L.) was one of the herbal plants whose fruit has long been used for the treatment and prevention of various diseases. This study aimed to determine the potential inhibition of white pomegranate leaf extracts (Punica granatum L.) on the growth of Gram-negative bacteria including Escherichia coli Extended-Spectrum Beta-Lactamase (ESBL) strain and Gram-positive bacteria Staphylococcus aureus Methicillin-Resistant Staphylococcus aureus (MRSA) strain. White pomegranate leaf extract macerated with ethanol 96%, evaporated to obtain pure extracts made with a concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and with 100% tested with invitro diffusion method. It was found that the extract of white pomegranate leaves with 30% (10.00 ± 0.0) concentration was able to inhibit the growth of positive Gram bacteria strains MRSA and the extract was unable to inhibit the growth of Escherichia coli bacteria strain ESBL.
Hepatitis B Virus Infection in Hemodialisis patient at Prof. DR. W.Z. Johannes Kupang Hospital, East Nusa Tenggara Irfan Irfan; Aris Wawomeo; Norma Tiku Kambuno
JURNAL KESEHATAN PRIMER Vol 4 No 1 (2019): JKP (Jurnal Kesehatan Primer)
Publisher : Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/jkp.v4i1.262

Abstract

Background: Hepatitis B or serum hepatitis is a liver infection caused by the hepatitis B virus (VHB) a member of the Hepadnavirus family that can lead to acute and chronic liver inflammation that can continue to be liver cirrhosis or liver cancer. Purpose: This research aims to determine the description of hepatitis B in hemodialysis patients in the Hospital Prof. Dr. W. Z. Johannes Kupang. Methods: This research is a descriptive study. Sampling was done by purposive sampling technique and a total sample of 63 hemodialysis patients. The inclusion criteria of the sample were those had got hemodialysis therapy> 6 months. Data was taken by interview based on a questionnaire to determine the history of hemodialysis, frequency of hemodialysis, history of blood transfusion and examination of blood serum by researchers who were accompanied by laboratory instructors. Results: The results showed that the infection of hepatitis B in hemodialysis patients in hospital Prof. Dr. W.Z. Johannes Kupang from 63 samples obtained 7 positive/reactive HBsAg (11%). Conclusion: History of hemodialysis, frequency of hemodialysis and history of blood transfusion were factors that have the potential to be a source of hepatitis B infection.
Pengaruh Paparan Pencemar Udara Terhadap Stres Oksidatif: Sistematik Review Kusmiyati Kusmiyati; Norma Tiku Kambuno; Pius Selasa; Ferry William Frangky Waangsir
Jurnal Ilmu Lingkungan Vol 20, No 3 (2022): July 2022
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.20.3.628-636

Abstract

Pencemaran udara mempengaruhi kualitas lingkungan dan kesehatan masyarakat.  Pencemar udara dapat menyebabkan perubahan molekul dalam tubuh sebagai penanda dini gangguan kesehatan. Tujuan penelitian ini adalah memperoleh gambaran tentang pengaruh pencemar udara terhadap stres oksidatif. Jenis penelitian adalah sistematik review dari artikel penelitian yang dilakukan sebelumnya. Penelitian dilaksanakan pada Apri-Mei 2021. Pencarian literature dilakukan melalui data base Science Direct pada periode publikasi 2017-2021.  Kata kunci yang digunakan adalah “air pollutant” OR “oxidative stress” AND “air pollution” OR “oxidative stress”.   Artikel yang diperoleh menggunakan kata kunci dan memenuhi kriteria inklusi selanjutnya dilakukan penilaian kelayakan menggunakan The JBI Critical Appraisal.  Dari menilaian kelayakan diperoleh 15 artikel dari data base Science Direct yang selanjutnya ditelaah. Hasil pdari penelusuran literature diketahui jenis pencemar udara antara lain PM2,5, NO2, Ozon, partikulat, PM10, PM2.5, NO2, dan CO, PM udara ambien, abu vulkanik, bahan bakar dan sebagainya. Paparan polutan udara dapat menyebabkan stress oksidatif dalam berbagai penanda antara lain peningkatan produksi spesies oksigen reaktif, penurunan antioksidan, 8-OHDG.  Kesimpulan dari penelitian ini bahwa pajanan pencemar udara menyebabkan perubahan pada penanda biologis stress oksidatif yang merupakan indikasi dini adanya gangguan kesehatan sebagai dampak dari pencemar udara.  Perlu upaya pengendalian pencemaran udara agar tidak menimbulkan dampak negatif pada manusia.ABSTRACTAir pollution had an impact on environmental quality and public health. Air pollutants could cause molecular changes in the body as an early marker of health problems. The purpose of this study was to obtain an overview of the effect of air pollutants on oxidative stress. This study was a systematic review of previous research articles. The research was carried out in April-May 2021. The literature search was carried out through the Science Direct database in the 2017-2021 publication period. The keywords used were “air pollutant” OR “oxidative stress” AND “air pollution” OR “oxidative stress”. Articles obtained using keywords and meeting the inclusion criteria were then assessed for feasibility using The JBI Critical Appraisal. From the feasibility assessment, 15 articles were obtained which were then reviewed. The results of a literature search showed that the types of air pollutants include PM2.5, NO2, Ozone, particulates, PM10, PM2.5, NO2, and CO, ambient air PM, volcanic ash, fuel and so on. Exposure to air pollutants caused oxidative stress in various markers, including increased production of reactive oxygen species, decreased antioxidants, 8-OHDG. The conclusion of this study is that exposure to air pollutants causes changes in biological markers of oxidative stress which is an early indication of health problems as a result of air pollutants. Efforts are needed to control air pollution so that it does not have a negative impact on humans.
Konseling Keluarga Pasien TB dan Pemberdayaan Pemuda Gerakan Anti-TB di Desa Naibonat Kecamatan Kupang Timur Kabupaten Kupang, Nusa Tenggara Timur Norma Tiku Kambuno; Ni Made Susilawati; Adrianus Ola Wuan; Novian A. Yudhaswara; Karol Octrisdey; Neiny P. Foekh; Yustina K. Wawo Aja
E-Dimas: Jurnal Pengabdian kepada Masyarakat Vol 11, No 3 (2020): E-DIMAS
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/e-dimas.v11i3.4831

Abstract

Tuberkulosis (TB) masih merupakan masalah penyakit menular di Indonesia termasuk Nusa Tenggara Timur. Desa Naibonat, Kabupaten Kupang Timur dilaporkan masih dengan angka insiden yang tinggi. Penyakit ini disebabkan oleh bakteri basil yang dikenal dengan nama Mycobacerium tuberculosis. Penularan melalui udara saat pasien batuk dan mengeluarkan droplet, anggota keluarga merupakan kelompok yang sangat rentan tertular karena tidak bisa menghindari kontak secara langsung. Penyakit TBC dapat disembuhkan dengan pengobatan teratur dan tidak putus selain itu pencegahan dapat dilakukan dengan menjaga sanitasi lingkungan, peningkatan daya tahan tubuh anggota keluarga, dukungan dari anggota keluarga kepada pasien yang sedang menjalani pengobatan sampai sembuh. Konseling keluarga dan pemberdayaan pemuda untuk menurunkan angka penularan TB kami nilai sebagai salah satu strategi yang efektif. Kegiatan inilah yang kami laksanakan dalam kegiatan pengabdian masyarakat dengan fokus pada wilayah layanan Puskesmas Naibonat, wilayah gereja katolik Gereja Katolik Sto. Yohanes Maria Vianey-Naibonat dan SMAN 3 Kabupaten Kupang Timur.
Factors Affecting the Incidence of Filariasis in Welamosa Village Ende District East Nusa Tenggara Irfan Irfan; Norma Tiku Kambuno; Israfil Israfil
Global Medical & Health Communication (GMHC) Vol 6, No 2 (2018)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (520.132 KB) | DOI: 10.29313/gmhc.v6i2.3208

Abstract

Filariasis is a chronic communicable disease caused by filarial worms, which consists of three species: Wucherria bancrofti, Brugaria malayi, and Brugaria timori. This disease is transmitted through mosquito bites, infects lymph tissue (lymph) and causes swelling of the legs, breasts, arms and genital organs. Welamosa village, Ende district, located in East Nusa Tenggara (NTT) province is reported as one of the highest cases of 40 cases in 2015. This research aims to analyze the influence of social factor of demography and socio-cultural environment factor to elephantiasis incident in Welamosa village, Ende district. The study was conducted in July–September 2016 in Welamosa village and Wolowaru sub-district, Ende district. The type of research was observational analytic with case-control with 49 people as sampling. The research instrument used questionnaire and check list. The data analysis used statistical test of SPSS program with backward regression logistic test. The results showed five variables as risk factors of elephantiasis occurrence, age (OR=42.518), education (OR=38.248), occupation (OR=8.404), outdoor activity at night (OR=5.097) and sex (OR=0.193). In conclusion, social demographic factors (age, gender, occupation, and education) and environmental and social-cultural factors of attitude (outdoor activities at night) are risk factors for filariasis incidence in Welamosa village, Ende district. FAKTOR YANG MEMENGARUHI KEJADIAN PENYAKIT FILARIASIS DI DESA WELAMOSA KABUPATEN ENDE NUSA TENGGARA TIMURFilariasis adalah penyakit menular menahun yang disebabkan oleh parasit berupa cacing filaria yang terdiri atas tiga spesies, yaitu Wucherria bancrofti, Brugaria malayi, dan Brugaria timori. Penyakit ini menginfeksi jaringan limfe (getah bening) dan menular melalui gigitan nyamuk, serta menyebabkan pembengkakan kaki, tungkai, payudara, lengan, dan organ genital. Desa Welamosa, Kabupaten Ende terletak di Provinsi Nusa Tenggara Timur (NTT) dilaporkan sebagai salah satu kecamatan dengan kasus filariasis tertinggi, yakni 40 kasus pada tahun 2015. Tujuan penelitian adalah menganalisis pengaruh faktor sosial demografi dan faktor lingkungan sosial budaya terhadap kejadian filariasis di Desa Welamosa, Kabupaten Ende. Penelitian dilaksanakan pada bulan Juli–September 2016 di Desa Welamosa dan Kecamatan Wolowaru, Kabupaten Ende. Jenis penelitian merupakan analitik observasional dan pengambilan sampel menggunakan case control sebanyak 49 orang. Instrumen penelitian menggunakan kuesioner dan ceklis. Analisis data menggunakan uji statistik program SPSS dengan backward regression logistic test. Hasil penelitian menunjukkan lima variabel yang merupakan faktor risiko kejadian filariasis, yaitu usia (OR=42,518), pendidikan (OR=38,248), pekerjaan (OR=8,404), aktivitas di luar rumah pada malam hari (OR=5,097), dan jenis kelamin (OR=0,193). Simpulan, faktor sosial demografi (usia, jenis kelamin, pekerjaan, dan pendidikan) serta faktor lingkungan sosial budaya sikap (aktivitas di luar rumah pada malam hari) merupakan faktor risiko terhadap kejadian filariasis di Desa Welamosa, Kabupaten Ende. 
Risk Factors of Intra-familial Hepatitis B Virus Transmission among Hepatitis B Patients in Kupang, Indonesia Norma Tiku Kambuno; Meylani Fernanda Bessie; Marni Tangkelangi; Agustina Welhelmina Djuma
Global Medical & Health Communication (GMHC) Vol 7, No 2 (2019)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (904.389 KB) | DOI: 10.29313/gmhc.v7i2.4149

Abstract

Hepatitis B is caused by acute or chronic hepatitis B virus infection. It is the most dangerous liver disease compared to other liver diseases due to its lack of apparent symptoms. The symptoms include slight jaundice in the eyes and skin accompanied by lethargy. This study aimed to determine the risk factors for intra-familial transmission of hepatitis B virus for household contacts of hepatitis B patients. The analytical correlation study with a cross-sectional design was conducted from June to July 2018 in Alak subdistrict, Kupang, Indonesia. Venous blood was collected from 45 subjects consisting of 12 patients and 33 family member. Examination was then performed using HBsAg test strip, resulting in the percentage of transmission of 15.15%. Statistical analysis revealed p>0.05. In conclusions, no relationship between gender, age, education, marital status, occupation, and HBsAg status. These characteristics are not risk factors for conversion of HBsAg status. FAKTOR RISIKO PENULARAN VIRUS HEPATITIS B KONTAK SERUMAH DI ANTARA PASIEN HEPATITIS B DI KUPANG, INDONESIAHepatitis B disebabkan oleh infeksi virus hepatitis B yang bersifat akut atau kronik. Penyakit ini termasuk penyakit hati yang paling berbahaya dibanding dengan penyakit hati yang lain karena tidak menunjukkan gejala yang jelas. Gejalanya hanya sedikit warna kuning pada mata dan kulit disertai lesu. Penelitian ini bertujuan mengetahui faktor risiko penularan virus hepatitis B pada kontak serumah pasien hepatitis B. Penelitian korelasi analitik dengan desain cross-sectional ini dilaksanakan pada bulan Juni sampai Juli 2018 di Kecamatan Alak, Kupang, Indonesia. Darah vena diambil dari 45 subjek yang terdiri atas 12 pasien dan 33 anggota keluarga. Pemeriksaan kemudian dilakukan menggunakan strip tes HBsAg menghasilkan persentase penularan 15,15%. Analisis statistik didapatkan p>0,05. Simpulan, tidak terdapat hubungan antara jenis kelamin, usia, pendidikan, status pernikahan, pekerjaan, dan status HbsAg. Karakteristik ini bukan faktor risiko untuk konversi status HBsAg.
Empowerment of Sto.Yohanes Maria Vianey Catholic Church Youth Naibonat of East Kupang, East Nusa Tenggara as A Supervision of Tuberculosis Ingestion Novian A Yudhaswara; M Ibraar Ayatullah; Novelia G So o; Irwan Budiana; Norma Tiku Kambuno
ABDIMAS: Jurnal Pengabdian Masyarakat Vol. 4 No. 2 (2021): ABDIMAS UMTAS: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM Universitas Muhammadiyah Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.122 KB) | DOI: 10.35568/abdimas.v4i2.1283

Abstract

Tuberculosis (TB) is an infectious disease that causes death, and is the third leading cause of death in Indonesia. The increase in cases of tuberculosis infection is still a major health problem in Naibonat Village, Kupang Regency, East Nusa Tenggara Province. Treatment failure, among others, was caused by patient non-compliance in ingesting drugs. It is hoped that the presence of supervisors to swallow drugs for TB patients is expected to help reduce TB infection cases. In Indonesian, a person who supervisors to swallow drugs is called “PMO” or “Pengawas menelan obat (PMO)” . Church Youth is an educated / educated group that is expected to have the ability to function as PMO. The purpose of this activity is to carry out PMO training for STO. Yohanes Maria Vianey Naibonat church youth through the method of lectures, discussions and microscopic training. The level of knowledge was measured using a questionnaire in the pre-test and post-test training. Youth who have been trained will be directed to become PMO’s for their families and closest neighbors who suffer from TB as well as agents of change in changing healthy behavior to prevent TB infection. The results of the activity showed a change in the level of knowledge before and after training from72% enough category to be good 89%. It was concluded that empowering church youth as PMO is an effort to suppress the increase in TB cases. The provision of PMO training can increase level of knowledge about TB so that regular and scheduled training will increase the ability and understanding of the role of PMO in reducing the incidence of TB in the community.
Co-Authors Agustina W. Djuma Agustina Welhelmina Djuma Akrom, Akrom Anita Dwi Putri Galla Aris Wawomeo Arnawa, I Gede Putu Ayatullah, M. Ibraar DAVID HANDOJO MULJONO Dicky Fanggidae Donald Emilio Kalonio Elisabeth N Barung Elisabeth N. Barung Elisabeth Patricia Febriani Jonison Fanggidae, Dicky Ferry WF Waangsir Herlinalt Gloria Banunu, Ayorince Irfan Irfan Irwan Budiana Israfil, Israfil Karol Octrisdey Khusnul Dwi Istnaini Kusmiyati Kusmiyati LOGA, MARIA CARITAS N.A.I Loisa Ratna Yuvita Olla MARIA CARITAS N.A.I LOGA Marni Tangkelangi Meylani Fernanda Bessie Muh. Nasrum Massi Neiny Prisy Foekh Ni Made Susilawati Novelia G So o Nurdin, Kuntum Ekawati Pius Selasa Rahmahsari Bala Sherly Dewu Wuan, Adrianus Ola Yos Banne Yunita Helmina Senge Yustina K. Wawo Aja