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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan
Pratitis, Asri
Research and Development Center for Marine and Fisheries Product Processing and Biotechnol
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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STUDY ON THE EFFECT OF POLLUTANTS ON THE PRODUCTION OF AAPTAMINES AND THE CYTOTOXICITY OF CRUDE EXTRACT FROM Aaptos suberitoides Dewi, Ariyanti Suhita; Hadi, Tri Aryono; Januar, Hedi Indra; Pratitis, Asri; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 3 (2012): December 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i3.4

Abstract

This experiment was conducted to study the effects of anthropogenic stressor on the spatialvariability of secondary metabolites from marine sponge Aaptos suberitoides. Samplings wereconducted at 7 sites in Marine National Park of Thousand Islands that are extended within 30 kmoff Jakarta bay on late February 2011. Sponges were collected and quantified by means of liquidchromatography coupled with photo-diode array detection, whereas, cytotoxicity of sponges extractswas determined against T47D (breast) cancer cell lines. Results showed that the spatialproduction of aaptamine and isoaaptamine did not correlate with the quality of their surroundinghabitat, despite nitrite and nitrate levels significantly affected the bioactivity of crude extracts.
IDENTIFICATION AND CULTIVATION OF MFW 23-08 ISOLATED FROM MARINE SPONGES FOR BIOACTIVE COMPOUND PRODUCTION Chasanah, Ekowati; Pratitis, Asri; Mangunwardoyo, Wibowo
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 2 (2012): August 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i2.16

Abstract

Production of marine bioactive compound for commercial usage has been hampered due tothe problem of raw material supply. To overcome this, marine microbes especially those associatedwith the bioactive-compound producer biota, has been explored as bioactive sources, with severaladvantages such as shorter production time, cheaper production cost and avoiding over exploitationof marine biota sources. Previous research showed that fungi MFW 23-08 was one of the potentialisolates from Wakatobi sponges which produced bioactive compounds that was active againstbreast cancer cell line and as antioxidant. This study was intended to identify MFW 23-08 andoptimize the production of its bioactive compound through optimization of MFW 23-08 culture.Culture optimization was conducted using 3 liquid media, i.e. malt extract broth (MEB), glucosepeptone yeast (GPY), and minimal fungal media (MFM), and cultivation periods, i.e. 2, 4, 6, 8, and10 wk. Results revealed that MFW 23-08 crude extract of 2 wk-MFM cultivation, at the concentrationof 30 μg/ml, was able to retard 87% breast cancer (T47D) cell growth. While at concentration of100 μg/ml, the 6 wk. MEB cultivated extract was able to hamper free radicals (56%). However, thecrude extract from MFM media cultivation, in the concentration of 50 and 100 μg/ml was not able toinhibit Escherichia coli and Staphylococcus aureus growth. Based on molecular identificationusing ITS1-ITS4 primers, MFW 23-08 isolate was 99% similar to  Penicillium citrinum, P.griseofolvum and Penicillium  sp.
Composition of Fatty Acids in Evaluation of Sea Cucumber Potency for Nutraceutical Product Development Susilowati, Rini; Pratitis, Asri; Januar, Hedi Indra
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 2 (2016): August 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i2.238

Abstract

Unsaturated fatty acid in food has an effect to improve human health, while saturated fatty acid has the opposite impact. Ratio between both types of fatty acids is important to evaluate the biological material for nutraceutical product development. This paper aimed to evaluate the nutraceutical potency of sea cucumber from Kendari Bay Waters, South East Sulawesi, based on their fatty acids composition. The analysis was conducted by the 1H-NMR (Hidrogen-Nuclear Magnetic Resonance) method. Several species within 4 genera (Holothuria, Bohadschia, Actinopyga, and Stichopus) were selected as the subject of the study. Comparison of fatty acid composition has been done in genera level, the preliminary study found that variation between species among the same genera was insignificant (p >0.05). Results of the study detected that Holothuria contained with an even ratio of Poly Unsaturated Fatty Acids (PUFA) and Saturated Fatty Acids (SFA), but Actinopyga was detected with low PUFA/SFA ratio. It may suggest that Holothuria from this region is the most potential raw material for nutraceutical product development. Meanwhile, precaution is needed for genera that contained with low PUFA/SFA ratio. Furthermore, comparison with other studies detected that fatty acids composition is not morphological specific, but vary, according to food viability, environmental and geographic location. Therefore, preliminary screening is important in evaluation of sea cucumber material for nutraceutical product development.  
Produksi Senyawa Bioaktif dari Aspergillus ustus MFW 26-08 yang Berasosiasi dengan Spons Laut dalam Berbagai Media Pratitis, Asri; Patantis, Gintung; Mangunwardoyo, Wibowo; Chasanah, Ekowati
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 5, No 2 (2010): Desember 2010
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v5i2.411

Abstract

Penggunaan mikroba sebagai sumber senyawa bioaktif memiliki beberapa kelebihan di antaranya mempersingkat waktu produksi dan menghindari pemanfaatan sumberdaya laut secara berlebih. Penelitian terdahulu menghasilkan beberapa isolat mikroba yang berpotensi sebagai penghasil senyawa bioaktif, di antaranya adalah isolat kapang MFW 26-08. Penelitian ini bertujuan untuk melakukan optimasi pertumbuhan isolat kapang MFW 26-08 dan produksi senyawa bioaktif yang disekresikan oleh kapang tersebut. Optimasi dilakukan dengan menggunakan 3 jenis media: Malt Extract Broth (MEB), Glucose Peptone Yeast (GPY), dan Minimal Fungal Media(MFM); serta waktu kultivasi 2, 4, 6, 8, dan 10 minggu. Hasil riset menunjukkan bahwa ekstrak kasar kapang MFW 26-08 hasil kultivasi 2 minggu dalam medium MFM, pada konsentrasi 30 µg/mL, mampu menghambat 89% pertumbuhan sel kanker payudara T47D. Sedangkan pada konsentrasi 100 µg/mL ekstrak kapang yang dikultivasi dalam medium MEB selama 6 minggu, mampu menghambat pembentukan radikal bebas sampai 56%. Hasil identifikasi berdasarkan sifat-sifat morfologi dan molekuler menggunakan 18S rRNA, ITS1, dan ITS4 menunjukkan bahwa isolat MFW 26-08 memiliki kemiripan dengan Aspergillus ustussebesar 99%
Co-Authors ARIYANTI SUHITA DEWI Ekowati Chasanah Gintung Patantis HEDI INDRA JANUAR Rini Susilowati, Rini Tri Aryono Hadi WIBOWO MANGUNWARDOYO