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EXPLORATION AND SELECTION OF RHIZOBACTERIA THAT INHIBIT PHYTOPHTHORA CAPSICI IN VITRO Zakia, Aulia; Ilyas, Satriyas; Budiman, Candra; ., Syamsuddin; Manohara, Dyah
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 18, No 1 (2018): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Exploration and Selection of Rhizobacteria that Inhibit Phytophthora capsici in vitro. Phytophthora capsici, a seed borne and the soil borne fungal pathogen is the cause of phytophthora blight on chili. The disease is difficult to control because of the resistant varieties unavailability in Indonesia. The aimed was to obtain isolates of rhizobacteria which has the ability to inhibit P. capsici in vitro. Rhizobacteria exploration was conducted in the chili production center in East Java (Malang, Batu, and Kediri) and West Java (Bogor). In one location, chili plant that had symptoms of phytophthora blight disease and a healthy plant next to it were chosen as samples to isolate P. capsici and the rhizobacteria. The rhizobacteria were isolated on NA, TSA, and TSAP (TSA with heated sample). Samples of diseased plants were used in isolation of P. capsici on V8 agar. The inhibition and compatibility of the rhizobacteria to inhibit P. capsici in vitro were tested by dual culture method. In this experiment, it was obtained 252 isolates of rhizobacteria and one isolate of P. capsici. Isolates of rhizobacteria with high to medium inhibition were E1, E3C2, and F2B1 respectively. All three isolates were then combined and tested against P. capsici in vitro. The highest inhibition was indicated by four isolate and combination of isolates, which were E1 isolate (58%), the combination of E1 + E3C2 isolates (58%), E1 + F2B1 (60%) and E1 + E3C2 + F2B1 (58 %).

Efektivitas Frekuensi dan Volume Penyemprotan Daun dengan Agens Hayati Filosfer dalam Meningkatkan Pertumbuhan Tanaman dan Hasil Padi Samsi Abdul Khodar; Satriyas Ilyas; dan Candra Budiman
Indonesian Journal of Agronomy Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTIncreased of plant productivity in field determined by the use of quality seed from improved variety. The purpose of this research was to study the effect of frequency and volume of phyllosphere biological agents by foliar spray on rice plant infected by Xanthomonas oryzae pv. oryzae (Xoo) in improving plant growth, production of rice seed, and controlling bacterial leaf blight (BLB). This research was conducted at Seed Health Laboratory, Department of Agronomy and Horticulture, Bogor Agricultural University, and Muara Experimental Field, Rice Research Institute, March through September 2014. The experiment was conducted using a randomized complete block design with two factors, and replicates as blocks. The first factor was five levels of frequency of spraying with biological agents: P0 = control, P1 = Xoo seed inoculated then invigorated with biomatriconditioning, P2 = Xoo inoculated seed, then biomatriconditioned, and the plants were sprayed with phyllosphere biological agents F112 once, P3 = as P2, and the plants were sprayed with F112 twice, and P4 = as P2, and the plants were sprayed with F112 three times. The second factor was three levels of spray volume: i.e., 300, 400, and 500 L ha-1. Result of this experiment revealed that biomatriconditioning with rhizobacteria P. diminuta A6 and B. subtilis 5/B improved field emergence and seedling dry weight. Seeds applied with biomatriconditioning then followed by foliar spray with phyllosphere bacteria F112 at 40, 60, and 80 days after transplanting effectively reduced severity of BLB, increased plant dry weight and seed yield. All treatments increased yield as compared to untreated control. Volume of foliar application showed no significant difference, therefore, the lowest volume of 300 L ha-1 was recommended. Keywords: Bacillus subtilis 5/B, biomatriconditioning, Pseudomonas diminuta A6, Xanthomonas oryzae pv. oryzae

Pengembangan Metode Uji Cepat Vigor Benih Kedelai dengan Pemunculan Radikula Feny Astuti; Candra Budiman; Satriyas Ilyas
Indonesian Journal of Agronomy Vol. 48 No. 2 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Pengujian vigor untuk mengevaluasi mutu benih memerlukan metode yang lebih cepat, tepat, dan mudah. Uji pemunculan radikula merupakan metode pengujian vigor yang telah divalidasi ISTA sejak tahun 2014 pada benih jagung. Penelitian ini bertujuan untuk mengembangkan metode uji RE pada benih kedelai dengan menentukan waktu pengamatan yang tepat serta mengkorelasikan uji RE dengan vigor benih lainnya, viabilitas, dan vigor bibit di lapangan. Penelitian dilaksanakan di Laboratorium Penyimpanan dan Pengujian Mutu Benih serta Kebun Pengujian vigor untuk mengevaluasi mutu benih memerlukan metode yang lebih cepat, tepat, dan mudah. Uji pemunculan radikula atau radicle emergence (RE) merupakan metode pengujian vigor benih yang cepat dan telah divalidasi International Rules for Seed Testing sejak tahun 2014 pada benih jagung. Penelitian ini bertujuan untuk mengembangkan metode uji RE sebagai uji cepat vigor pada benih kedelai. Penelitian dilaksanakan di Laboratorium Penyimpanan dan Pengujian Mutu Benih serta Kebun Percobaan Sawah Baru IPB pada bulan November 2018 - Maret 2019. Rancangan kelompok lengkap teracak (RKLT) dengan pengamatan berulang pola split-plot digunakan pada percobaan uji RE, dengan sepuluh varietas sebagai petak utama dan tujuh waktu pengamatan sebagai anak petak. Rancangan percobaan RKLT dengan satu faktor yaitu varietas digunakan untuk pengujian vigor benih dan daya berkecambah di laboratorium, dan vigor bibit di lapangan. Penentuan waktu pengamatan RE yang tepat dilakukan dengan interval 2 jam mulai dari 36 jam setelah pengecambahan (JSP) hingga 48 JSP. Hasil uji RE dikorelasikan dengan hasil uji beberapa tolok ukur vigor lainnya, daya berkecambah, dan vigor bibit di lapangan. Hasil penelitian menunjukkan bahwa uji RE pada benih kedelai dari sepuluh varietas yang berbeda tingkat vigor (pemilahan berdasarkan nilai indeks vigor) dengan metode uji diatas kertas pada suhu 25 ± 2 °C dan diamati pada 42 jam ± 15 menit setelah pengecambahan berkorelasi erat dan dapat memprediksi daya berkecambah, indeks vigor, kecepatan tumbuh, rataan waktu perkecambahan, daya tumbuh di lapangan, dan rataan waktu pemunculan bibit. Oleh karena itu, RE dapat digunakan sebagai uji cepat vigor benih kedelai. Kata kunci: daya berkecambah, daya tumbuh, rataan waktu perkecambahan, rataan waktu pemunculan bibit

Efektivitas Seed Coating dan Biopriming dengan Rizobakteri dalam Mempertahankan Viabilitas Benih Cabai dan Rizobakteri selama Penyimpanan Ita Madyasari; Candra Budiman; Syamsuddin ,; Dyah Manohara; Satriyas Ilyas
Jurnal Hortikultura Indonesia Vol. 8 No. 3 (2017): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

ABSTRACTThe objective of the study was to obtain the best coating formula for hot pepper seeds, and evaluate the effect of seed coating and biopriming with rhizobacteria on viability of hot pepper seeds and rhizobacteria during storage. Experiment 1 was arranged in a completely randomized design with one factor i.e. 11 coating formula. Experiment 2 was arranged in a nested plot design with two factors, storage period (0, 4, 8, 12, 16, 20, and 24 weeks) as main factor and seed treatment consisted of 11 treatments (control, seed coating with E1+F2B1, ST116B, CM8; biopriming 24 h with E1+F2B1, ST116B, CM8; biopriming 48 h with E1+F2B1, ST116B, and CM8; priming metalaxyl) as nested factor. Result of experiment 1 indicated that the best coating formula for hot pepper seed was sodium alginate 2.5% and was used in experiment 2. Experiment 2 showed that seed coating and biopriming with rhizobacteria were able to maintain seed viability (79-89%) for 24 weeks of storage at 27-30 0C as compared to priming metalaxyl (54%). Biopriming E1+F2B1 24 h or CM8 48 h resulted in the highest index of seed vigor after 24 weeks of storage. Population of rhizobacteria in seed tissue decreased in bioprimed seeds from 105-107 cfu g-1 to 104 cfu g-1 after being stored for 24 weeks. Keywords: rhizobacteria isolates, seed treatment, seed vigor, sodium alginate ABSTRAK Penelitian ini bertujuan mendapatkan formula coating terbaik pada benih cabai dan mengevaluasi pengaruh seed coating dan biopriming dengan rizobakteri dalam mempertahankan viabilitas benih cabai dan rizobakteri selama penyimpanan. Percobaan 1 menggunakan rancangan acak lengkap satu faktor yang terdiri atas 11 formula coating. Percobaan 2 menggunakan rancangan petak tersarang dua faktor, periode simpan (0, 4, 8, 12, 16, 20, dan 24 minggu) sebagai faktor utama dan perlakuaan benih yang terdiri atas 11 perlakuan (kontrol, seed coating dengan E1+F2B1, ST116B, CM8; biopriming 24 jam dengan E1+F2B1, ST116B, CM8; biopriming 48 jam dengan E1+F2B1, ST116B, dan CM8; priming metalaksil) sebagai faktor tersarang. Hasil Percobaan 1 menunjukkan bahwa formula coating terbaik untuk benih cabai ialah natrium alginat 2.5% dan digunakan pada percobaan 2. Percobaan 2 menunjukkan bahwa seed coating dan biopriming dengan rizobakteri mampu mempertahankan viabilitas benih (78-89%) selama 24 minggu penyimpanan pada suhu 27-30 0C dibandingkan priming metalaksil (54%). Biopriming E1+F2B1 24 jam atau biopriming CM8 48 jam menghasilkan indeks vigor paling tinggi setelah disimpan selama 24 minggu. Populasi rizobakteri di dalam jaringan benih menurun pada benih yang diberi perlakuan biopriming dari 105-107 cfu g-1 menjadi 104 cfu g-1 setelah disimpan selama 24 minggu.Kata kunci: isolat rizobakteri, natrium alginat, perlakuan benih, vigor

Peningkatan Pertumbuhan Tanaman Cabai dan Pengendalian Busuk Phytophthora melalui Biopriming Benih dengan Rizobakteri Asal Pertanaman Cabai Jawa Timur Aulia Zakia; Satriyas Ilyas; Candra Budiman; Syamsuddin ,; Dyah Manohara
Jurnal Hortikultura Indonesia Vol. 8 No. 3 (2017): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

ABSTRACT The objectives of this study was to evaluate biopriming of chili seed with rhizobacteria to improve plant growth and control Phytophthora blight disease in a greenhouse. This experiment used three isolates of rhizobacteria, i.e. E1, E3C2 and F2B1, and isolate Phytophthora capsici (Cb6) isolated from the production center of chili in East Jawa. Laris variety from PT. East West was used in this experiment. This experiment used randomized block design with one factor, i.e. 11 levels of seed treatment (E1 rhizobacteria, E3C2 rhizobacteria, F2B1 rhizobacteria, E1+E3C2 rhizobacteria, E1+F2B1 rhizobacteria, E1+E3C2+F2B1 rhizobacteria, seed soaking in water, without soaking, metalaxyl, positive control and negative control). The result showed that seed treatment with combination of E1+F2B1 isolates when grown in nursery, significantly increased the height and number of leaves in chilli. Besides, seed treatment with F2B1 isolate and combination of E1+F2B1 isolates after transplanting were capable to improve plant growth and control Phytophthora blight disease in greenhouse.Keywords: greenhouse, isolate rhizobacteria, Phytophthora capsici ABSTRAK Tujuan penelitian ini ialah mengevaluasi perlakuan biopriming benih cabai dengan rizobakteri dalam meningkatkan pertumbuhan bibit dan mengendalikan kejadian busuk Phytophthora di rumah kaca. Perlakuan biopriming benih dengan rizobakteri menggunakan tiga isolat rizobakteri E1, E3C2 dan F2B1 dan isolat Phytophthora capsici Cb6 hasil eksplorasi pertanaman cabai Jawa Timur. Benih yang digunakan dalam percobaan merupakan benih varietas Laris produksi PT. East West. Percobaan menggunakan rancangan acak kelompok satu faktor, masing-masing perlakuan diulang empat kali, dengan 11 taraf perlakuan, antara lain R0+ (kontrol positif, benih direndam dalam PDB tanpa perlakuan rizobakteri dengan inokulasi P. capsici), R0- (kontrol negatif, benih direndam dalam PDB tanpa perlakuan rizobakteri dan tanpa inokulasi P. capsici), R1 (perlakuan benih dengan isolat E1), R2 (isolat E3C2), R3 (isolat F2B1), R4 (kombinasi isolat E1+E3C2), R5 (kombinasi isolat E1+F2B1), R6 (kombinasi isolat E1+E3C2+F2B1), R0RA (benih direndam dalam air 24 jam), R0TR (benih tanpa rendam), R0M (benih direndam dalam metalaksil). Tanah inokulum P. capsici diberikan 28 hari setelah pindah-tanam di sekitar pangkal batang tanaman cabai di bawah permukaan tanah. Hasil percobaan menunjukkan, perlakuan dengan kombinasi isolat E1+F2B1 saat persemaian di rumah kaca nyata meningkatkan tinggi dan jumlah daun tanaman cabai. Perlakuan benih dengan isolat F2B1 maupun kombinasi isolat E1+F2B1 setelah pindah-tanam di rumah kaca memiliki kemampuan meningkatkan pertumbuhan tanaman serta mengendalikan penyakit busuk Phytophthora. Kata kunci: isolat rizobakteri, Phytophthora capsici, rumah kaca

Pembelajaran Partisipatif Secara Daring bagi Petani Sorgum di Kabupaten Ende, Nusa Tenggara Timur Ridwan Diaguna; Okti Syah Isyani Permatasari; Candra Budiman; Ahmad Zamzami; Vincencius Arman; Joni Aba; Flora Ifoni Naomi
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 8 No. 1 (2022): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.8.1.113-119

Dryland is a potential food production in the future and sorghum is one of the potential commodities to be developed. Sorghum has been cultivated by a small number of people in Ende Regency, such as in Kotabaru and Nangapanda sub-districts. However, the cultivation has not yet applied cultivation techniques to achieve production optimization. In addition, there are still many limitations in knowledge of processing the production of sorghum seeds, leaves, and stems. Farmers have not enjoyed and received the benefits of cultivation so far. The purpose of this study is to increase awareness, understanding, and knowledge about the importance of sorghum to support food security and farmer welfare in Ende Regency, and to map the potential of online learning for farmers. The online learning was carried out in Kotabaru Village with the target being the Kema Sa Ate Women Farmers Group (KWT), which are sorghum cultivators. Learning is carried out using the lecture plus method (lecture-discussion) using a zoom meeting. Learning materials about harvesting and post-harvesting sorghum. The obstacle faced in this online learning is a device that does not support it. This problem was solved by involving a learning facilitator played by Field Agricultural Extension (PPL). Participants' initial knowledge before the training program was 3.8 and after training the final knowledge was 7.2. Based on the initial and final knowledge, it can be concluded that there is an increase in knowledge of 89.5%. The level of participant satisfaction with the 5 indicators proposed in the evaluation of the learning implementation process is very high, more than 80%. The level of participants' satisfaction with the five indicators in the evaluation of the training process also increased. More than 90% for problem solving in the field, speaker competence, and the level of urgency of information, while for media innovation and training methods more than 80%.

PENGARUH PERLAKUAN BENIH PADI (Oryza sativa L.) YANG TERINFEKSI Xanthomonas oryzae PV. Oryze TERHADAP PERTUMBUHAN TANAMAN DAN HASIL DI RUMAH KACA Candra Budiman; Satriyas Ilyas
Hexagro Journal Vol. 2 No. 1 (2018)
Publisher : Universitas Perjuangan Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/hexagro.v2i1.117

Penggunaan benih bermutu dari varietas unggul merupakan faktor utama dalampeningkatan produksi tanaman di lapangan. Penelitian bertujuan untuk menemukanperlakuan benih yang dapat meningkatkan mutu pertumbuhan dan produksi padi dirumah kaca. Percobaan menggunakan Rancangan Petak Terbagi dengan petak utama,yaitu varietas (IR64 dan Ciherang), dan anak petak perlakuan benih : kontrol, bakterisidasintetik (Agrept 0.2% b/v), minyak serai wangi (1% v/v), agens hayati (Pseudomonasdimunuta skala 4 Mc Farland), matriconditioning + bakterisida sintetik (streptomisin sulfat0.2% b/v), matriconditioning + minyak serai wangi (1% v/v), matriconditioning + agenshayati (Pseudomonas dimunuta skala IV Mc Farland). Dari penelitian ini didapatkan bahwapengaruh perlakuan benih meningkatkan petumbuhan tanaman pada fase awalpertumbuhan. Pada fase lebih lanjut, pengaruh varietas lebih berpengaruh. Varietasciherang secara umum lebih produktif dan tahan terhadap HDB dibanding varietas IR64.Perlakuan matriconditioning + streptomisin sulfat 0.2% dan matriconditioning P. dimunutameningkatkan daya berkecambah, tinggi tanaman dan jumlah anakan.Kata kunci : Ciherang, IR6, matriconditioning, perlakuan benih

Pemanfaatan Larva Black Soldier Fly (Hermatia illucens) sebagai Penanggulangan Sampah Organik melalui Budidaya Magot Fifi Fata'tiatul Hidayah; Destya Nurfrida Rahayu; Candra Budiman
Jurnal Pusat Inovasi Masyarakat (PIM) Vol. 2 No. 4 (2020): Juni 2020
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (452.916 KB)

Tuwel Village is the village with the most population and automatically the amount of organic waste donated by the community also increases. Organic waste is exacerbated by the absence of a Temporary Disposal Site in the village. Environmental pollution that occurs in the form of an unpleasant odor arises as a result of spoilage of organic material from waste. Maggot is one of the potential organisms that can break down organic waste. The village community does not have knowledge about maggot cultivation which can provide benefits. The purpose of this program is to inform the public about organic waste management and increase the economic value of the community through waste management activities. The method used is the socialization and designation of maggot metamorphosis. Both activities were carried out on two different days with 3 different places. The result of this program is that the community understands the life cycle of maggot, the procedures for cultivating maggot, the benefits in cultivating maggot, a type of organic waste that can be processed by maggot. Organic waste is useful as a medium for maggot cultivation. During the activity, participants actively listened and asked questions in the question and answer session. Keywords: cultivation, maggot, organic waste, socialization

Sosialisasi Pemilahan Sampah dan Pelatihan Pembuatan Kompos di Desa Luwijawa, Kecamatan Jatinegara, Kabupaten Tegal Rifqie Mardiansyah Purmadi; Rahmi Jamza; Sugeng Santoso; Fitrianingrum Kurniawati; Budi Nugroho; Candra Budiman
Jurnal Pusat Inovasi Masyarakat (PIM) Vol. 2 No. 4 (2020): Juni 2020
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (227.686 KB)

Luwijawa Village is a village located in Jatinegara District, Tegal Regency, Central Java Province. The village which has an area of 257,285 ha, the majority of its residents work as farmers. Agriculture carried out is rice farming with rice and corn as the main crops, as well as plantation crops such as coffee and durian. Villagers still use chemical fertilizer as a nutrient provider for plants, as well as the problem of rubbish that is still burned or thrown away by residents. The activity of sorting waste and training on composting was carried out to educate the public, especially PKK women, on how to manage waste to compost fertilizer. The socialization is done by the lecture method. The speaker explained the material using a power point and continued with video playback. Participants can ask questions after the socialization activities have been completed. Socialization activities and compost making training were carried out on different days. Compost making training is conducted with residents from organic waste. Organic waste obtained from residents' kitchen waste is cut to pieces and mixed with bioactivators (EM- 4). The ingredients that have been mixed are then put into a bucket and tightly closed so that no air enters, this is done to maximize the work of the bioactivator in the fermentation process. Compost making training activities took place well, there were quite a lot of participants, with relatively high enthusiasm. This is evidenced by the many questions asked by participants in the series of activities. Participants have been able to distinguish organic and inorganic waste, and have the desire to make their own compost with materials available in the surrounding environment. Keywords: compost, garbage, socialization

PEMUPUKAN BAHAN ORGANIK UNTUK PERTUMBUHAN DAN HASIL UMBI TALAS SUTRA Ridwan Diaguna; Edi Santosa; Candra Budiman; Ahmad Zamzami; Okti Syah Isyani Permatasari; Aldi Kamal Wijaya
Jurnal AGROSAINS dan TEKNOLOGI Vol 7, No 1 (2022)
Publisher : Fakultas Pertanian - UMJ

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jat.7.1.35-42

Indonesia has a great taro diversity and had been utilized for long time. Taro has more competitiveness including high nutrition, wide adaptation, and ease for cultivation, further, it’s very potential to be the future food and create new economical value. The objective of the research was to obtain the organic manure fertilizer effect on sutra taro variety growth and yield. The research was conducted for eight months from January till August 2021 at Leuwikopo Farm, Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University. A Completely Randomized Block Design (CRBD) with five replications and four-level organic manure treatments (0.25 kg, 0.5 kg, 0.75 kg, and 1.00 kg per plant) was applied. Taro growth, corm characters, and yields were affected by organic manure fertilizer dose. The dose of 0.75-1.00 kg/plant (equivalent to 15-20 ton/ha) showed the highest growth, corm characters and yields more than the lower doses. Taro “Sutra variety” cultivation by fully using organic manure fertilizers is very potential to be encouraged.

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