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All Journal Biosaintifika: Journal of Biology & Biology Education
Wahidah, Etty Nurul
Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro
Agriculture, Biological Sciences & Forestry

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Expression of Mycobacterium tuberculosis Protein Tyrosine Phosphatase B in Escherichia coli and Its Recovery from Inclusion Body Savalas, Lalu Rudyat Telly; Sedijani, Prapti; Hadisaputra, Saprizal; Ardhuha, Jannatin; Lestari, Chomsa Asih; Wahidah, Etty Nurul
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.12384

Abstract

The present study aims at expressing and partially purifying PtpB in active form. To achieve this, Mtb PtpB gene has been cloned into pET30a vector and overexpressed in Escherichia coli BL 21(DE3) under IPTG induction in the form of an inclusion body. Following resolubilization by urea and dialysis, the resulted PtpB has been shown to be active against para-Nitrophenyl phosphate. It is concluded that the resulted PtpB has had been recovered from inclusion body to give the active form of the enzyme, and thus the success in overexpressing PtpB provides the required material to investigate the biochemical properties of the pathogen virulence factor further.
Co-Authors JANNATIN ARDHUHA, JANNATIN LALU RUDYAT TELLY SAVALAS Lestari, Chomsa Asih PRAPTI SEDIJANI, PRAPTI Saprizal Hadisaputra