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All Journal Jurnal Teknologi Laboratorium Jurnal Ilmu dan Teknologi Kesehatan JPP Jurnal Kesehatan Poltekkes Palembang International Journal of Advanced Health Science and Technology STRADA Jurnal Ilmiah Kesehatan Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat
Woelansari, Evy Diah
Poltekkes Kemenkes Surabaya
Chemistry Computer Science & IT Dentistry Electrical & Electronics Engineering Health Professions Medicine & Pharmacology Nursing Public Health Veterinary Other

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Imunomodulator Ekstrak Etanol Daun Mimba (Azadirachta indica) terhadap Jumlah Sel Makrofag Peritoneal pada Mencit yang Diinduksi Vaksin BCG Abror, Yogi Khoirul; Woelansari, Evy Diah; Suhariyadi, Suhariyadi
Jurnal Teknologi Laboratorium Vol 7 No 1 (2018): 2018 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (837.953 KB) | DOI: 10.29238/teknolabjournal.v7i1.110

Abstract

This research was conducted to determine the immunomodulatory effect of ethanol extract of neem leaves (Azadirachta indica) to the number of peritoneal macrophages in mice wich induced by BCG vaccine. Bacillus Calmette-Guerin (BCG) vaccine contained an attenuated Mycobacterium bovis. Mycobacterium bovis belongs to the Mycobacterium Tuberculosis Complex (MTC) group that has a similar phenotype characteristic with Mycobacterium tuberculosis and similar clinical manifestations of tuberculosis.The type of the research that used in this study is laboratory experimental research with Post Test Design Design Only Control Group Design. The research was conducted at the Faculty of Veterinary Medicine of Airlangga University in July 2017 using 25 male mice divided into five groups. The dosage of ethanol extract of the neem leaves given was 200 mg / Kg BW with variation for two days, four days, and six days are given.In the result of statistical data analysis using Kruskal-walis test, it is known that the significance value p = 0,03 (p <0,05), that means immunomodulatory of ethanol extract of neem leaves (Azadirachta indica) give an effect to peritoneal macrophage cell number in mice wich induced by BCG vaccine, so that neem leaves ethanol extract can be applied to tuberculosis patients.
POLA PERTUMBUHAN Staphylococcus aureus PADA MEDIA AGAR DARAH MANUSIA GOLONGAN O, AB, DAN DARAH DOMBA SEBAGAI KONTROL Evy Diah Woelansari
Jurnal Ilmu dan Teknologi Kesehatan Vol 3 No 2 (2016): Maret
Publisher : Poltekkes Kemenkes Jakarta III

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Abstract

Blood Agar Plate (BAP) with sheep blood is the medium used for the identification and isolation of the bacterium Staphylococcus aureus. Because to produce is difficult, but is needed in health education institutions, BAP then used media as a human blood substitute. This study was to observe the pattern of growth of Staphylococcus aureus in media BAP human blood group O, AB and the blood of the sheep as a controle. With the aim to test the feasibility of using human blood group O and AB as essential compounds in the manufacture of a blood substitute sheep BAP media. Staphylococcus aureus growth patterns observed macroscopically to colony morphology, the number of colonies, and the diameter of the haemolysis zone. The suspension of bacteria used were pure cultures of Staphylococcus aureus (ATCC 25923) which is synchronized with Mc Farland 0.5 Standart solution, then do thinning 10-2. The suspension of bacteria that are already experiencing thinning planted in the media BAP human blood group O, AB and sheep blood each at 9 petri dish.From the observation of colony morphology, the number of colonies, and the diameter of the zone of haemolysis performed statistical tests using Kruskal Wallis test values obtained Asymp. Sig of 0.352 for the number of colonies and haemolysis zone diameters greater than α (0.05), which means there is no significant difference between the growth pattern of Staphylococcus aureus in media BAP using human blood group O, AB and sheep blood as a medium BAP controle.
SEROPREVALENSI ANTIBODI IgGToxoplasma gondii PADA IBU DI RANGKAH 6 SURABAYA Dwi Krihariyani Krihariyani; Evy Diah Woelansari; Entuy Kurniawan Kurniawan
Jurnal Ilmu dan Teknologi Kesehatan Vol 3 No 1 (2015): September
Publisher : Poltekkes Kemenkes Jakarta III

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Abstract

Toxoplasmosis is zoonotic diseases caused Toxoplasma gondii and can infected women. Risk factor Toxoplasmosis infection in women are habits of washing hand after contact wih animal. Thepurpose of the study was to research seroprevalence antibody IgG Toxoplasma gondii among women in Rangkah 6 Surabaya. This research is observational purposive sampling study.Total sample amount 45 sample from women and analyzed antibody IgG Toxoplasma gondii using ELISA technique. The result showed 57,5% (26/40) seropositive antibody IgG Toxoplasma gondii whereas42,5% (14/40) seronegative antibody IgG Toxoplasma gondii. Seropositive 6 of 26 women (23,1%) is pregnant women. Seronegative IgG T.gondii 5 from 14 (35,7%) is pregnant women.These research with Chi-Square test was level value p=0.976that meansno significantrelations between antibody IgG Toxoplasma gondii with habits of washing hand.The prevalence of Toxoplasma gondii antibody among women is high in Rangkah 6 Surabaya. these research will be neededAppropriated to screen women before and during pregnancy, should be dealt in order to minimize of this infection.
Korelasi antara Trombositopenia Imunoglobulin M dan Imunoglobulin G pada Anak yang Menderita Dengue Haemorrhagic Fever Hariyanto Hariyanto; Evy Diah Woelansari; Suliati Suliati
STRADA Jurnal Ilmiah Kesehatan Vol. 7 No. 1 (2018): May
Publisher : Institut Ilmu Kesehatan STRADA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.077 KB) | DOI: 10.30994/sjik.v7i1.142

Abstract

DHF merupakan demam berdarah dengue yang disebabkan oleh virus dengue yang ditularkan oleh nyamuk Aedes aegypti. Demam Berdarah Dengue dengan gejala umum demam, nyeri sendi, lekopenia dengan salah satunya trombositopeni. Demam akibat infeksi virus menyebabkan respon imun yaitu IgM dan IgG yang berasal dari limfosit B. Penelitian ini bertujuan untuk membuktikan adanya hubungan antara trombositopeni dengan imunoglobulin M dan imunoglobulin G pada anak yang menderita DHF. Penelitian ini bersifat deskriptif analitik yang dilakukan antara bulan Januari sampai Juni 2013 di laboratorim RSU Dr. Iskak Tulungagung. Dari pemeriksaan terhadap 60 kasus yang mengalami penurunan trombosit < 100.000/mm3 atau trombositopeni terdapat hasil IgG yang reaktif 23 (38,3%) IgG yang nonreaktif 14 (23,3%) IgM yang reaktif 26 (43,3%) IgM yang non reaktif 11 (18,3%) yang negatif IgG dan IgMnya walaupun trombositopeni sebanyak 23 (28,3%) dari hasil penelitian lanjutan dengan statistik dilakukan uji non parametrik corelation didapatkan p = 0,000 pada IgG dan p = 0,000 pada IgM artinya p < α. Artinya ada hubungan antara trombositopenia dengan imunoglobulin M dan imunoglobulin G pada anak yang menderita dengue haemorhagic fever. Korelasi yang terjadi antara yang terjadi adalah korelasi yang kuat dan searah artinya apabila trombosit naik maka IgM dan IgG naik atau sebaliknya. Untuk hubungan IgG dan IgM didapat nilai r = 0,590 pada α = 0,01. Artinya hubungan IgG dan IgM mempunyai kuat hubungan sedang dan searah.
KORELASI NILAI LAJU ENDAP DARAH (LED) DENGAN HIGH SENSITIVITY C-REACTIVE PROTEIN (hs-CRP) PADA PEROKOK AKTIF DI WARUNG KOPI WILAYAH SURABAYA TIMUR Rieke Dwi Ramadhany; Evy Diah Woelansari; Christ Kartika Rahayuningsih; Nadi Aprilyadi
JPP JURNAL KESEHATAN POLTEKKES PALEMBANG Vol 17 No 2 (2022): JPP (Jurnal Kesehatan Poltekkes Palembang)
Publisher : POLITEKNIK KESEHATAN KEMENKES PALEMBANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/jpp.v17i2.1297

Abstract

Background Smoking has become one of the leading causes of death worldwide. The negative impact of smoking usually occurs after a few years when a person starts actively smoking can harm the body's organs and become a major risk factor for diseases with inflammatory components. Systemic inflammatory reactions are characterized by an increase in inflammatory cytokines, blood cell counts and blood viscosity. Examination CRP and ESR are the two most frequently measured laboratory tests in assessing the response of a systemic inflammatory process. This study aims to determine and analyze the correlation between ESR values ​​and hs-CRP in active smokers at coffee shops, East Surabaya. Methods: The type of research used is correlation research with cross sectional. The sample used is 30 active smokers who meet the inclusion criteria. This research was carried out on February 21 – April 11, 2022. The examination of ESR values ​​and hs-CRP levels was carried out at Balai Besar Laboratorium Kesehatan (BBLK) Surabaya. Results: It was found that 30 respondents had been actively smoking >5 years with a percentage of 100%. as many as 8 respondents consumed 1-10 cigarettes/day with a percentage of 27%, 19 respondents consumed 10-20 cigarettes per/with a percentage of 63%, and 3 respondents consumed >20 cigarettes/day with a percentage of 10%. The average value of ESR is 8.37 mm/Hr and The The average value of the of hs-CRP 1.11 mg/L. Conclusion: Based on statistical tests, There is no correlation between the value of ESR and hs-CRP in active smokers in a coffee shop in the East Surabaya area (p value = 0.099) with a sufficient relationship and a positive direction of relationship (r = 0.307).
Expression of Immunoglobulin G / Immunoglobulin M Typhoid On Neutrophil-Lymphocyte Ratio In Patients At Haji Hospital, Surabaya Nur Zerlinda; Evy Diah Woelansari; Anita Dwi Anggraini
International Journal of Advanced Health Science and Technology Vol. 3 No. 4 (2023): August
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v3i4.289

Abstract

Typhoid fever is one of the major bacterial infections worldwide caused by the bacterium Salmonella enterica serovars typhi in humans. The examination used is the rapid Typhoid test. This rapid typhoid test is used to detect antibodies to Salmonella typhi. The purpose of the study was to determine the results of the Neutrophil - Lymphocyte Ratio with various criteria results from the Immunoglobulin G / Immunoglobulin M Test in typhoid fever patients. This type of study is descriptive observational with selective sampling taken. The samples in this study were 28 samples conducted in April 2023 on typhoid fever patients at the Hajj Hospital in East Java Province. This study used the Rapid Typhoid Test Immunochromatography method with positive Widal criteria and supporting examination using Neutrophil–Lymphocyte Ratio (NLR) with Hematology Analyzer tool Fluorescent Flow Cytometry method. Typhoid and NLR rapid test results are processed descriptively in tables and based on percentages. The results obtained from the rapid test were positive IgM of 29%, positive IgG of 11%, positive IgG and IgM of 3%, and negative IgG and IgM of 57%. The average NLR was 4,43% with a median of 2,85. IgG / IgM examination of NLR showed no significant association in typhoid fever patients. It is hoped that this research will be further developed using Salmonella culture examination.
Correlation Between HIV Rapid Test and HIV Viral Load in HIV Patients at Haji Hospital in Surabaya Resty Cahya Pertiwi; Evy Diah Woelansari; Ayu Puspitasari
International Journal of Advanced Health Science and Technology Vol. 3 No. 4 (2023): August
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v3i4.290

Abstract

HIV (Human Immunodeficiency Virus) is a virus that causes decreased immunity. Examinations that can be done for HIV testing include a rapid HIV test and HIV viral load. The rapid HIV test is a test that is used to find out the antibodies in the sample, while the viral load test is to find out the amount of HIV. The purpose of this study was to compare HIV using a rapid test with HIV viral load in people with HIV. This type of research is observational with a cross-sectional approach. The sample in this study were 30 samples from HIV sufferers who carried out an HIV viral load examination using the Molecular Rapid Test (TCM) method at the RSUD Haji Provinsi Jawa Timur, then a rapid HIV test was carried out using the immunochromatography method. The results of the HIV rapid test and HIV viral load were processed in the form of a bar chart and tested with the McNemar statistical test. The results of the McNemar test can be concluded that there is a significant difference between the results of the HIV viral load examination of HIV viral load by the Molecular Rapid Test (TCM) method is superior to the HIV rapid test. However, rapid tests can be used in diagnosing HIV infection.
Kolesterol Dan Tekanan Darah Lansia Di Posyandu Abiyoso Polkesbaya Juliana Christyaningsih; Luthfi Rusyadi; Minarti; Mamik; Pestariati; Diah Titik Mutiarawati; Suliati; Retno Sasongkowati; Evy Diah Woelansari; Suhariyadi; Wisnu Istanto; Lully Hanni Endarini; Museyaroh
JURNAL RAKAT SEHAT (JRS) : Pengabdian Kepada Masyarakat Vol 2 No 2 (2023): Jurnal Rakat Sehat: Pengabdian Kepada Masyarakat
Publisher : UPPM Poltekkes Kemenkes Banjarmasin

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Abstract

As people age, the physical state of the human body will gradually deteriorate due to a decrease in the quantity of cells present, leading to a gradual reduction in functionality referred to as the aging process. In elderly humans (aged more than 60 years) excessive cholesterol levels and high blood pressure are one of the causes of diseases such as heart disease which has been recognized by WHO as the number one cause of death in the world. The buildup of fatty deposits on the linings of blood vessels has the potential to lead to obstructions within the blood vessels. The objective of this initiative is to enhance understanding regarding the hazards associated with cholesterol. This outreach effort took place in July 2023, conducted at Posyandu Abiyoso, Surabaya Health Polytechnic. The activity consists of several processes 1). conducting a survey of the location of the Abiyoso posyandu of the Surabaya Health Polytechnic, 2). Opening registration and taking participants' personal data, 3) conducting participant health interviews, 4). Checking cholesterol levels and blood pressure of participants. From the community service activities carried out, it was found that 42 of the 65 (64.6%) participants who took part in the examination at the integrated service post had high cholesterol levels, and 60 of the 65 (92.3%) participants had high blood pressure or hypertension. By conducting health checks on cholesterol levels and blood pressure, there is an expectation that the society will come to realize and comprehend. the dangers of hypertension and hypercholesterolemia so that the community can prevent the occurrence of sustainable diseases.
Co-Authors A.A. Ketut Agung Cahyawan W Abror, Yogi Khoirul Anita Dwi Anggraini Aprilyadi, Nadi Ayu Puspitasari Christ Kartika Rahayuningsih Diah Titik Mutiarawati Dwi Krihariyani Entuy Kurniawan Kurniawan Hariyanto Hariyanto Juliana Christyaningsih Lully Hanni Endarini Luthfi Rusyadi Mamik MINARTI Museyaroh Nur Zerlinda Pestariati Pestariati Resty Cahya Pertiwi Retno Sasongkowati Rieke Dwi Ramadhany Suhariyadi Suhariyadi Suhariyadi Suliati Suliati Suliati Wisnu Istanto