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Antioxidant activity of methanolic extract from yellow paprika (Capsicum annuum, L.) by DPPH radical scavenging method Warsi, Warsi; Guntarti, Any
Pharmaciana Vol 7, No 2 (2017): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (549.708 KB) | DOI: 10.12928/pharmaciana.v7i2.3905

Abstract

Yellow paprika (Capsicum annuumL.) is one of fruit that contains of carotenoid compounds as an antioxidant. This reasearch was aimed to know an antioxidant capacity of methanolic extract from yellow paprika with 2,2–diphenyl–1–picrylhydrazyl (DPPH) radical scavenging method. The fresh yellow paprika was determined of its loss of drying by gravimetric. The yellow paprika extract was obtained by maceration using methanol as solvent. The content of β–carotene from this extract was analyzed qualitively by thin layer chromatography and UV-Vis spectrophotometer. The antioxidant activity of methanolic extract from yellow paprika was determined base on measurement of residual DPPH by UV–Vis spectrophothometric at wavelength of 515.6 nm. The data was used to calculate % inhibition. Furthermore, IC50 value was determined to know potency of its antioxidant. The result of loss of drying yellow paprika fruit was obtained 6.33 ± 0.02 %. The analysis with thin layer chromatography and UV-Vis spectrophotometer showed that this fruit was contain of β–carotene. The result of antioxidant activity analysis from yellow paprika fruit was obtained IC50 value of 0.3028 ± 0.0093 mg/mL. The result of the research showed that the antioxidant potency of yellow paprika was less than β–carotene (IC50 0.0852 ± 0.0009 mg/mL).
Aktivitas Penangkapan Radikal 2,2–Difenil–1–Pikrilhidrazil (DPPH) Oleh Ekstrak Metanol Paprika Merah (Capsicum annuum, L.) Warsi, Warsi; Guntarti, Any
Media Farmasi: Jurnal Ilmu Farmasi Vol 13, No 1: Maret 2016
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (555.865 KB) | DOI: 10.12928/mf.v13i1.5740

Abstract

Paprika merah (Capsicum annum L.) banyak mengandung β–karoten  (provitamin A), likopen, vitamin E serta vitamin C telah diuji aktivitasnya sebagai penangkap radikal 2,2–difenil–1–pikrilhidrazil (DPPH). Sampel diekstraksi dengan cara maserasi menggunakan metanol. Ekstrak yang diperoleh dilakukan analisis aktivitas antioksidannya secara kualitatif dengan larutan DPPH 0,4 mM dan reagen ferri tiosianat. Identifikasi terhadap senyawa yang berpotensi sebagai antioksidan dilakukan dengan spektrofotometer UV–Vis. Aktivitas penangkapan radikal DPPH oleh ekstrak metanol paprika merah ditetapkan secara spektrofotometri pada panjang gelombang 517 nm. Dari hasil analisis diperoleh nilai IC50 paprika merahsebesar0,299 ± 4,98x10-3 mg/mL. Aktivitas paprika merah sebagai penangkap radikal DPPH  kurang poten dibandingkan dengan β–karoten (IC50 0,066 ± 3,24x10-3 mg/mL). Kata kunci : antioksidan, paprika merah, Capsicum annuum, DPPH