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Sperm motility in chronic bacterial prostatitis Dicky Moch Rizal, Dicky Moch Rizal
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 35, No 2 (2003)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Background: Male infertility is 40% couples problem in conceiving a child. One of the causes of male infertility that has not studied yet is chronic bacterial prostatitis (CBP). CBP can decrease sperm motility that affects potency of sperm in fertilizing the oocyte. Another effect of CBP is leucospermia as the unique phenomenon in male accessory gland infection (MAGI).Objectives: To describe and to obtain the relation between sperm motility in CBP and the other prostate dysfunction that is not included to CBP (non CBP).Methods: This study was analytic descriptive non experimental study. The-subjects were person having suffered from prostate dysfunction, including CBP and non CBP, in Infertility Clinic Permata Hati of DR. Sardjito hospital. Data were taken from expressed prostatic secretion (EPS) analyses and seminal fluid analyses from May 2001 until June 2002 (56 data), Variable that observed from seminal fluid analyses were sperm motility and leucocyte count per milliliter. Determination of the CBP case was based on leucocyte count per high power field (HPF) and the other characteristics (i.e : clumping leucocytes and lipid laden macrophage) of EPS analyses. Data was analyzed by descriptive frequency to show the distribution of sperm motility and leucospermia. Beside to notice the relation between prostate dysfunction and sperm motility, Chi-Square test was used to notice the relation between prostate dysfunction and leucospermia as well.Results: As much as 76.7% patients with CBP had sperm motility disruption in progressive linear and linear movement. Fifty percents patients with prostate dysfunction had sperm motility disruption in progressive linear and linear movement. Mean of sperm motility With progressive linear movement in CBP and prostate dysfunction non CBP were 18.65% and 22.58%. Mean of sperm motility with linear movement in CBP and prostate dysfunction non CBP were 38.79% and 46.83%. From the Chi-Square test we obtained the significant difference between sperm motility with progressive linear and linear movement of the sperm and prostate dysfunction. -Conclusions: Over a half of patients (76.7%) with CBP suffered from decreased sperm motility. CBP affected sperm motility.Key words: male infertility, sperm motility, chronic bacterial prostatitis, semen analysis, expressed prostatic secretion (EPS)

Expression of receptor advanced glycosilation end product (RAGE) and active caspase-3 of the streptozotocin-induced chronic diabetes mellitus Sprague Dawley ratsâ sperm with soybean (Glycin max) powder suspension treatment Sri Kadarsih Soejono, Mustofa Dicky Moch Rizal
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 01 (2013)
Publisher : Universitas Gadjah Mada

Diabetes mellitus (DM) affects all the process of spermatogenesis. Chronic hyperglycemia in DM increases the expression of receptor for advanced glycosilation end products (RAGE) that is responsible for the activation of signal production of reactive oxygen species (ROS) and caspase 3. Active caspase 3 plays an important role in cell apoptosis. Soybean (Glycin max) is reported to have antihyperglycemic and antiadvanced glycosilation end products (antiAGE) and antioxidants activities. The aim of this study was to evaluate the effect of soybean powder suspension on the expression of RAGE and active caspase 3 of diabetic ratsâ sperm. This was an experimental study with post test only control group design using 30 male Sprague Dawley rats, aged 11-12 weeks old and weighed 200-250g. The rats were divided into five groups with six rats in each group. Group 1 was non diabetic rats andÂ Group 2 was diabetic rats that were given aquadest. Group 3-5 were diabetic rats that were given a soybean powder suspension at dose of 400; 800 and 1600 mg/kg body weight (BW)/day, respectively. Diabetic rats were made by induction of a single intraperitoneal injection of streptozotocin (STZ) at a dose of 60 mg/kg BW. Soybean powder suspension was ingested for four weeks after 14 days STZ induction. Blood glucose levels were monitored before and three days after STZ induction and four weeks after suspension ingestion. The expression of RAGE and active caspase-3 were analyzed using immunohistochemistry method four weeks after suspension ingestion. The results showed that soybean powder suspension ingestion significantly decreased blood glucose level of diabetic rats toward normality (p<0.05). However, the expression of RAGE and active caspase-3 in diabetic ratsâ sperm were not significantly lower than those after suspension ingestion. In conclusion, soybean powder suspension does not significantly affect the expression of RAGE and active caspase-3 in diabetic ratsâ sperm.

Serum testosterone level and active caspase-3 of Leydig cells of diabetic Sprague-Dawley male rats after administration of soybean (Glycin max) powder suspension Sri Kadarsih Soejono, Wiyadi Dicky Moch. Rizal
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.014 KB)

Diabetes mellitus (DM) is known as the main death cause in the world. This disease causes acuteand chronic complication. The common chronic complication on male reproduction system isthe decrease of testosterone level. This hormone is produced in the Leydig cells. Soybeans(Glycin max) has been used in the management of DM to maintain blood glucose level. However,the effect of soybean on serum testosterone level is still unclear. The aim of this study was toevaluate serum testosterone level and caspase-3 active of Leydig cells of diabetic Sprague-Dawley (SD) male rats model induced by streptozotocine (STZ) after oral ingestion of soybeanpowder suspension. This was an experimental study with pre and post test control group design.Thirty SD male rats, aged 11-12 weeks with body weight (BW) of 200-250 g were divided into 5groups with 6 rats in each group. The first group (G1) was normal rat control and the secondgroup (G2) was diabetic rat control. The third to fifth group (G3, G4 and G5) were diabetic rattreatment with oral ingestion of soybean powder suspension that was given once a day during 4weeks with dose of 400; 800 and 1600 mg/kg BW, respectively. The serum testosterone levelwas measured by an ELISA and active caspase-3 of Leydig cells were measured by animmunohistochemistry method. The result showed that the serum testosterone level and activecasapse-3 Leydig cells of diabetic rats model induced by STZ were not significantly differentafter oral ingestion of soybean powder suspension with dose of 400, 800 and 1600 mg/kg BW(p>0.05). In conclusion, soybean powder suspension did not affect the serum testosteronelevel and active caspase-3 Leydig cells in diabetic rats.Keywords: diabetes - soybean - phytoestrogen - testosterone - active caspase-3

Relationship between hyperuricemia and erectile dysfunction on hypertension patients Vera Ticoalu, Jansje Henny; Kandarina, Istiti; Rizal, Dicky Moch.; Pramantara Setiabudi, I Dewa Putu
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 2 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Hypertension is a major non-communicable disease worldwide including in Indonesia. It can cuase erectile dysfunction through vasculogenic pathway. Uric acid level could be a promising biomarker to predict erectile dysfunction due to it is related to endothelial dysfunction, microvascular disease, and hypertension. The aim of this study was to evaluate the relationship between hyperuricemia and erectile dysfunction on hypertension patients. This was an observational study with cross-sectional design involving 88 male hypertension patients aged more than 18 years who registered as outpatient in the Bethesda Lempuyangwangi Public Hospital, Yogyakarta. Hyperuricemia was measured using uricase method and erectile dysfunction was measured using IIEF-5 form. The results showed that hyperuricemia (OR=3.89; CI 95% 1.08-15.70; p=0.017), blood pressure (OR=6.84; CI 95% 2.35-20.6; p<0.001), and age (p<0.001) are related with erectile dysfunction on the hypertension patients. Furthermore, logistic regression analysis showed that hyperuricemia, age, and blood pressure simultaneously affect the erectile dysfunction occurance, with good calibration (p=0.167) and discriminative level (0.8604). In conclusion, there is significantly relationship between hyperuricemia and erectile dysfunction on hypertension patients.

HUBUNGAN DESIRE DALAM AKTIVITAS SEKSUAL DENGAN HORMON PROGESTERON WANITA HAMIL TRIMESTER I, II DAN III Arif Tirtana; Ova Emilia; Dicky Moch. Rizal
Jurnal Kesehatan Madani Medika (JKMM) Vol 9 No 1 (2018): Jurnal Kesehatan Madani Medika
Publisher : Sekolah Tinggi Ilmu Kesehatan Madani Yogyakarta

Pregnant women will produce higher hormone progesteron in each trimester (TM), this is intended to maintain pregnancy. One function of the progesterone hormone in maintaining pregnancy is the progesterone hormone as immunomodulator. The hormone progesterone can suppress the activation of NK cells in pregnant women. The hormone progesterone in women serves as a sexual drive that in stimulating the existence of sexual activity (Desire). The purpose of this study was to determine the relationship of desire with progesterone hormone in pregnant women TM I, TM II and TM III. The research design was cross sectional. Sample calculation on research based on correlation coefficient (r) using total sample size formula. Evaluation of desire using Female Sexual Function Index (FSFI) questionnaire. Different tests use Kruskal Wallis. Correlation test using pearson. The results of different desire test in pregnant women TM I, TM II and TM III were 0,250 (P> 0,005). Different test results of progesterone hormone in pregnant women of trimester I, II and III with result 0.000 (p

The Effect of Ethanol Extract of Piper nigrum L. Fruit on Reproductive System in Adult Male Wistar Rats: A Study of FSH, LH, Testosterone Level and Spermatogenic Cells Ekaputri, Tia Wida; Sari, Ika Puspita; Rizal, Dicky Moch.
Indonesian Journal of Pharmacy Vol 29 No 3, 2018
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Exploration to find out new natural contraceptive agent for male is still being developed. Black pepper (Piper nigrum L.) and its main alkaloid piperine have potential antifertility because of sitotoxic and hormonal effects. The aim of this study is to find out the effect of ethanolic extract of black pepper (Piper nigrum L.) fruit of on reproductive hormone serum level, sperm quality, and spermatogenic cell populations in adult male Wistar rat. Twenty five male rats were divided into five groups consisting of two control group, i.e. K(-) (Na-CMC 0.5%), K(+) (finasteride 0.45mg/kg BW), and three groups received different doses of black pepper fruit ethanolic extract, i.e. D(1) (3.33mg/kg BW), D(2) (6.66mg/kg BW) and D(3) (13.32mg/kg BW) respectively. The treatment were given to each group for 55 days. Reproductive parameters were measured, including serum level of reproductive hormones (FSH, LH, dan testosteron), quality of cauda epididymal sperm (spermatozoa concentration, motility and morphological abnormality), spermatogenic cell populations (primary spermato-cyte and spermatid count) and seminiferous tubules diameter. Ethanol extract of black pepper fruit at doses of 3.33mg/kg BW, 6.66mg/kg BW, and 13.32mg/kg BW increased serum FSH level. Extract at dose of 13.32mg/kg BW decreased serum LH level, while extract at doses of 6.66mg/kg BW and 13.32mg/kg BW decreased serum testosterone level. The number of primary spermatocytes, spermatozoa concentration, and spermatozoa motility were decreased by administration of ethanol extract of black pepper fruit with dose of 6.66mg/kg BW and 13.32mg/kg BW. Ethanol extract of black pepper fruit at dose of 6.66mg/kg BW and 13.32mg/kg BW had a negative impact on the male reproductive system and showing potential antifertility in maleÂ rat.

Serum testosterone level and active caspase-3 of Leydig cells of diabetic Sprague-Dawley male rats after administration of soybean (Glycin max) powder suspension Wiyadi Dicky Moch. Rizal Sri Kadarsih Soejono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.014 KB)

Diabetes mellitus (DM) is known as the main death cause in the world. This disease causes acuteand chronic complication. The common chronic complication on male reproduction system isthe decrease of testosterone level. This hormone is produced in the Leydig cells. Soybeans(Glycin max) has been used in the management of DM to maintain blood glucose level. However,the effect of soybean on serum testosterone level is still unclear. The aim of this study was toevaluate serum testosterone level and caspase-3 active of Leydig cells of diabetic Sprague-Dawley (SD) male rats model induced by streptozotocine (STZ) after oral ingestion of soybeanpowder suspension. This was an experimental study with pre and post test control group design.Thirty SD male rats, aged 11-12 weeks with body weight (BW) of 200-250 g were divided into 5groups with 6 rats in each group. The first group (G1) was normal rat control and the secondgroup (G2) was diabetic rat control. The third to fifth group (G3, G4 and G5) were diabetic rattreatment with oral ingestion of soybean powder suspension that was given once a day during 4weeks with dose of 400; 800 and 1600 mg/kg BW, respectively. The serum testosterone levelwas measured by an ELISA and active caspase-3 of Leydig cells were measured by animmunohistochemistry method. The result showed that the serum testosterone level and activecasapse-3 Leydig cells of diabetic rats model induced by STZ were not significantly differentafter oral ingestion of soybean powder suspension with dose of 400, 800 and 1600 mg/kg BW(p>0.05). In conclusion, soybean powder suspension did not affect the serum testosteronelevel and active caspase-3 Leydig cells in diabetic rats.Keywords: diabetes - soybean - phytoestrogen - testosterone - active caspase-3

Expression of receptor advanced glycosilation end product (RAGE) and active caspase-3 of the streptozotocin-induced chronic diabetes mellitus Sprague Dawley rats’ sperm with soybean (Glycin max) powder suspension treatment . Mustofa; Dicky Moch Rizal; Sri Kadarsih Soejono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 01 (2013)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Diabetes mellitus (DM) affects all the process of spermatogenesis. Chronic hyperglycemia in DM increases the expression of receptor for advanced glycosilation end products (RAGE) that is responsible for the activation of signal production of reactive oxygen species (ROS) and caspase 3. Active caspase 3 plays an important role in cell apoptosis. Soybean (Glycin max) is reported to have antihyperglycemic and antiadvanced glycosilation end products (antiAGE) and antioxidants activities. The aim of this study was to evaluate the effect of soybean powder suspension on the expression of RAGE and active caspase 3 of diabetic rats’ sperm. This was an experimental study with post test only control group design using 30 male Sprague Dawley rats, aged 11-12 weeks old and weighed 200-250g. The rats were divided into five groups with six rats in each group. Group 1 was non diabetic rats and Group 2 was diabetic rats that were given aquadest. Group 3-5 were diabetic rats that were given a soybean powder suspension at dose of 400; 800 and 1600 mg/kg body weight (BW)/day, respectively. Diabetic rats were made by induction of a single intraperitoneal injection of streptozotocin (STZ) at a dose of 60 mg/kg BW. Soybean powder suspension was ingested for four weeks after 14 days STZ induction. Blood glucose levels were monitored before and three days after STZ induction and four weeks after suspension ingestion. The expression of RAGE and active caspase-3 were analyzed using immunohistochemistry method four weeks after suspension ingestion. The results showed that soybean powder suspension ingestion significantly decreased blood glucose level of diabetic rats toward normality (p<0.05). However, the expression of RAGE and active caspase-3 in diabetic rats’ sperm were not significantly lower than those after suspension ingestion. In conclusion, soybean powder suspension does not significantly affect the expression of RAGE and active caspase-3 in diabetic rats’ sperm.

Sperm motility in chronic bacterial prostatitis Dicky Moch Rizal Dicky Moch Rizal
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 35, No 2 (2003)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Background: Male infertility is 40% couples' problem in conceiving a child. One of the causes of male infertility that has not studied yet is chronic bacterial prostatitis (CBP). CBP can decrease sperm motility that affects potency of sperm in fertilizing the oocyte. Another effect of CBP is leucospermia as the unique phenomenon in male accessory gland infection (MAGI).Objectives: To describe and to obtain the relation between sperm motility in CBP and the other prostate dysfunction that is not included to CBP (non CBP).Methods: This study was analytic descriptive non experimental study. The-subjects were person having suffered from prostate dysfunction, including CBP and non CBP, in Infertility Clinic Permata Hati of DR. Sardjito hospital. Data were taken from expressed prostatic secretion (EPS) analyses and seminal fluid analyses from May 2001 until June 2002 (56 data), Variable that observed from seminal fluid analyses were sperm motility and leucocyte count per milliliter. Determination of the CBP case was based on leucocyte count per high power field (HPF) and the other characteristics (i.e : clumping leucocytes and lipid laden macrophage) of EPS analyses. Data was analyzed by descriptive frequency to show the distribution of sperm motility and leucospermia. Beside to notice the relation between prostate dysfunction and sperm motility, Chi-Square test was used to notice the relation between prostate dysfunction and leucospermia as well.Results: As much as 76.7% patients with CBP had sperm motility disruption in progressive linear and linear movement. Fifty percents patients with prostate dysfunction had sperm motility disruption in progressive linear and linear movement. Mean of sperm motility With progressive linear movement in CBP and prostate dysfunction non CBP were 18.65% and 22.58%. Mean of sperm motility with linear movement in CBP and prostate dysfunction non CBP were 38.79% and 46.83%. From the Chi-Square test we obtained the significant difference between sperm motility with progressive linear and linear movement of the sperm and prostate dysfunction. -Conclusions: Over a half of patients (76.7%) with CBP suffered from decreased sperm motility. CBP affected sperm motility.Key words: male infertility, sperm motility, chronic bacterial prostatitis, semen analysis, expressed prostatic secretion (EPS)

Adjustment of sperm cryopreservation laboratory management during the COVID-19 pandemic Dicky Moch Rizal
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 53, No 1 (2021)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

The COVID-19 pandemic has changed the order of life in all fields, particularlyin medical services. Laboratory services in sperm cryopreservation that partof andrology or in vitro fertilization (IVF) laboratory cannot be separated withhandling sperm from patients. Although the presence of viruses in semenhas been known through various studies, there is scarce evidence regardingwhether sperm fluid can be a medium of COVID-19 transmission. This raisedthe question of whether some adjustments to manage patient sperm shouldbe made. This review described an overview of the various efforts that can bemade to reduce the risk of disease transmission to health workers who handlepatient sperm fluid.

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