DALIMARTHA, LENGGA NURULLAH
Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

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Pengaruh Teknik Sterilisasi dan Komposisi Medium terhadap Pertumbuhan Tunas Eksplan Sirsak Ratu Sulistiyo, Rico Hutama; Luthfiyyah, Zayyan; Susilo, Buana; Dalimartha, Lengga Nurullah; Wiguna, Eko Chandra; Yuliana, Nuniek; Prasetyo, Endry Nugroho
Bioedukasi: Jurnal Pendidikan Biologi Vol 11, No 1 (2018): BIOEDUKASI: Jurnal Pendidikan Biologi
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.619 KB) | DOI: 10.20961/bioedukasi-uns.v11i1.19726

Abstract

Successful tissue culture of soursop plants requires proper sterilization techniques and hormone levels to produce good shoot growth. This study aims to determine the effect of sterilization techniques and medium composition of soursop explants, using factorial completely randomized design with 2 factors: sterilizing agents and medium composition, repeated 3 times. Hydrogen Peroxide, Mercury and Sodium Hypoclorite used as sterilizing agents. The medium composition used in this study consisted of 3 levels: MS 0, MS 0.5 ppm BAP + 0.05 ppm NAA and MS 1 ppm BAP + 0.1 ppm NAA. The best treatment is MS medium 0.5 ppm BAP + 0.05 ppm NAA with hydrogen peroxide sterilizing agent which produces the highest propagation level and lowest contamination level.