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ISOLASI BAKTERI PENGHASIL SELULOSA DARI BUAH-BUAHAN DIPASAR TRADISIONAL MAKASSAR Herwin, Herwin; Fitriana, Fitriana; Nurung, Ayyub Harly
Jurnal Ilmiah As-Syifaa Vol 12, No 1 (2020): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.237 KB)

Isolation of bacterial cellulose from fruits in traditional market of Makassar have been done. The objective of this research is to adding diversity of microorganism that can producing cellulose and observe it?s both optimum temperature and pH. First step is isolation of bacteria from Watermelon, Papaya, Cantaloupe, Mango, Dragon fruit, and Banana that found in traditional market in Makassar using Hestrin-Schramm (HS) agar. Each colony bacteria have been separated to making pure culture using HS agar then the colony was screening in HS broth to obtain colony bacteria that producing cellulose. Screening test found that 10 isolate that can produce cellulose which is MB-B02, MS-B01, MS-B03, NB-B03, PB-B01, PB-B02, PB-B03, PB-B04, PS-B01, and PS-B03. Then the colony that producing cellulose was optimize its temperature and pH. Cellulose obtained from optimization was measure it?s weight to determine optimum temperature and pH. Based from optimization found that 250C was optimum temperature for MS-B01, MS-B03, NB-B03, dan PB-B04, and 370 was optimum temperature for MB-B02, PB-B01, PB-B03, PS-B01, dan PS-B03, meanwhile for isolate PB-B02 optimum both in 250C dan 370C dan pH optimum for all isolate is pH 6

ANALISIS KOMPONEN KIMIA AKTIF ISOLAT DAUN Colocacia esculenta L. IDTK01 SECARA SPEKTROFOTOMETER INRA MERAH Herwin, Herwin; Baits, Muzakkir; Ririn, Ririn; Harly Nurung, Ayyub
Jurnal Ilmiah As-Syifaa Vol 13, No 1 (2021): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jifa.v13i1.746

The objective of this research is to analysis chemical coumpounds active isolate of Colocacia esculenta L.â€™s leaves IDTK01 using infra red spectrophotometer. The fractionation of the ethanolic extract of Colocasia esculenta Lâ€™s leaves using liquid vacuum chromatography obtained 7 fractions. Active fraction was B fraction isolated from the active coumpound by TLC-preparative from 3 different isolate, IDTK01, IDTK02 dan IDTK03. IDTK01 isolate was tested its antibacterial activity using TLC-bioautography with eluen n-hexane:ethyl acetate (10:1) and its active against Salmonella thypi with Rf value 0.98. Active isolate IDTK01 was analyze its active bacterial coumpound using infrared spectrophotometer and the results is hydrogen bond, hydroxyl functional group (OH), aryl/ aromatic (C=C), aliphatic (CH2), Ether aldehyde (C-O), and pyridine.

AKTIVITAS ANTIOKSIDAN EKSTRAK FERMENTAT ISOLAT FUNGI ENDOFIT BIJI BUAH KENARI (Canarium indicum L.) SECARA KLT-AUTOGRAFI Naim, Nurul Khatimah; Herwin, Herwin; Fitriana, Fitriana; Nurung, Ayyub Harly
As-Syifaa Jurnal Farmasi Vol 13, No 2 (2021): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jifa.v13i2.794

Walnut seeds (Canarium indicum L.) contain tannin, flavonoids, carotenoids which is potentially fungtioning as antioxidants. The research aimed to examine the antioxidant activity of fermentate extract of walnut seeds endophytic fungi by autography-TLC. The result of the endophytic fungi isolation in Walnut seeds (Canarium indicum L.) obtained 5 isolates with characteristics. The test result of screening free antiradical activity obtained that isolate 4th was active as an antioxidant indicated by the presence of brown spots with the addition of 1,1-diphenyl-2-picrylhydrazyl 0.004%. Isolate 4th was fermented using Maltosa Yeast Broth (MYB) medium to obtain fermentate supernatant and mycelia. The Fermentate of supernantant of isolate 4th was extracted to obtained supernantant of ethyl acetate extract. Based on the result of free antiradical activity test of ethyl acetate fermentate extract isolate 4th by autography-TLC using ethyl acetate: methanol: water eluent (6:2:1), obtained Rf 0.78 value active as free antiradical by spraying 1,1diphenyl-2-picrylhydrazil 0.004%.

AKTIVITAS ANTIOKSIDAN EKSTRAK FERMENTAT ISOLAT FUNGI ENDOFIT BIJI BUAH KENARI (Canarium indicum L.) SECARA KLT-AUTOGRAFI Nurul Khatimah Naim; Herwin Herwin; Fitriana Fitriana; Ayyub Harly Nurung
As-Syifaa Jurnal Farmasi Vol 13, No 2 (2021): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jifa.v13i2.780

Walnut seeds (Canarium indicum L.) contain tannin, flavonoids, carotenoids which is potentially fungtioning as antioxidants. The research aimed to examine the antioxidant activity of fermentate extract of walnut seeds endophytic fungi by autography-TLC. The result of the endophytic fungi isolation in Walnut seeds (Canarium indicum L.) obtained 5 isolates with characteristics. The test result of screening free antiradical activity obtained that isolate 4th was active as an antioxidant indicated by the presence of brown spots with the addition of 1,1-diphenyl-2-picrylhydrazyl 0.004%. Isolate 4th was fermented using Maltosa Yeast Broth (MYB) medium to obtain fermentate supernatant and mycelia. The Fermentate of supernantant of isolate 4th was extracted to obtained supernantant of ethyl acetate extract. Based on the result of free antiradical activity test of ethyl acetate fermentate extract isolate 4th by autography-TLC using ethyl acetate: methanol: water eluent (6:2:1), obtained Rf 0.78 value active as free antiradical by spraying 1,1diphenyl-2-picrylhydrazil 0.004%.

ISOLASI BAKTERI PENGHASIL SELULOSA DARI BUAH-BUAHAN DIPASAR TRADISIONAL MAKASSAR Herwin Herwin; Fitriana Fitriana; Ayyub Harly Nurung
As-Syifaa Jurnal Farmasi Vol 12, No 1 (2020): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Isolation of bacterial cellulose from fruits in traditional market of Makassar have been done. The objective of this research is to adding diversity of microorganism that can producing cellulose and observe it’s both optimum temperature and pH. First step is isolation of bacteria from Watermelon, Papaya, Cantaloupe, Mango, Dragon fruit, and Banana that found in traditional market in Makassar using Hestrin-Schramm (HS) agar. Each colony bacteria have been separated to making pure culture using HS agar then the colony was screening in HS broth to obtain colony bacteria that producing cellulose. Screening test found that 10 isolate that can produce cellulose which is MB-B02, MS-B01, MS-B03, NB-B03, PB-B01, PB-B02, PB-B03, PB-B04, PS-B01, and PS-B03. Then the colony that producing cellulose was optimize its temperature and pH. Cellulose obtained from optimization was measure it’s weight to determine optimum temperature and pH. Based from optimization found that 250C was optimum temperature for MS-B01, MS-B03, NB-B03, dan PB-B04, and 370 was optimum temperature for MB-B02, PB-B01, PB-B03, PS-B01, dan PS-B03, meanwhile for isolate PB-B02 optimum both in 250C dan 370C dan pH optimum for all isolate is pH 6

ISOLASI DAN IDENTIFIKASI FUNGI ENDOFIT BATANG SESURU (Euphorbia antiquorum L.) SEBAGAI PENGHASIL ANTIBAKTERI DENGAN METODE KLT-BIOAUTOGRAFI Asnita Asnita; Herwin Herwin; Rachmat Kosman; Ayyub Harly Nurung
As-Syifaa Jurnal Farmasi Vol 12, No 2 (2020): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jifa.v12i2.718

The research aimed to obtain antibacterial activity of isolate fermentate of endophytic fungi of antique spurge stem (Euphorbia antiquorum L.) on Staphylococcus aureus and Escherichia coli by TLC-Bioautography. The methods used in this research were isolation, purification and isolate screening. The screening results of endophytic fungi isolate of antique spurge stem showed the largest inhibitory zone in preventing both bacteria growth using code IFBS-03 and IFBS-06. The chromatogram profile of antibacterial activity isolate fermentate of the codes IFBS-03 and IFBS-06 showed active spots using TLC-Bioautographic method showed that code IFBS-03 isolate obtained Rf 0.87 and 0.27 values, indicating active spots on Staphylococcus aureus and Escherichia coli. The result of code IFBS-06 isolate obtained Rf 0.83 value active spots against Staphylococcus aureus and values Rf 0.83 and 0.27 value on Escherichia coli. Based on isolation and identification some chemical components obtained that dietyl ether ekstract of thin layer cromatography obtained chemicals components flavanoid at Rf 0.83 and 0.27 value with reagent Antimo (III) chloride.

EFFECT OF 1,2-EPOXY-3[3-3[3,4-DIMETOXYI-PHENIL]-4H-1-BENZOPIRAN-4-ON] PROPANE (EPI) ON SIRTUIN-1 AND NUCLEAR FACTOR-κB EXPRESSION OF MAMMARY TUMORS INDUCED IN SPRAGUE DAWLEY RATS BY DMBA Ayyub Harly Nurung; Sri Herwiyanti; Dewi Kartikawati Paramita
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 3 (2016)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

ABSTRACT The main factors contribute to breast cancer development is the combination of exogenous and endogenous factors. Endogenous factors include both SIRT1 and NF-kB. Exogenous factor used in this research is 7.12 dimethylbenz(a)anthracene (DMBA). 1,2-epoxy-3[3- 3[3,4-dimetoxy-phenil]-4h-1-benzophiran-4-on] propane (EPI) is a derivative of isoflavone generate from clove leaf oil. To examine the effect of EPI on SIRT1 and NF-kB expression in DMBA-induced Sprague Dawley (SD) rats, and the correlation between SIRT1 and NFkB expressions. Tissue generated form 35 Sprague Dawley female rats aged 2 weeks old were used in this study. Those rats were divided into 7 groups (5 rats/group), namely normal control group; corn oil group; DMBA group; EPI treated groups with 1 mg/kgBW (EPI I), 2 mg/kgBW (EPI II), and 4 mg/kgBW (EPI III), respectively; and doxorubicin group. EPI and doxorubicin were administered from 1st until 15th week, while DMBA were administered from 3rd until 9th week. The paraffin block was prepared from all breast organ of the rats that terminated at the end of week 15th. Examination of SIRT1 and NF-kB expression was performed using light microscope at 400x magnifications, after immunohistochemistry (IHC) staining. Expression level of SIRT1 and NF-kB were analyzed using IHC-profiler plug-in in ImageJ software. SIRT1 and NF-kB expression in EPI treated groups were not significantly different with the one in Doxorubicin group, but lower than DMBA group (p=0.000). There was a positive correlation between SIRT1 and NF-kB expression (p=0.001; r=0.773) in EPI-treated group. EPI was able to prevent an increasing of SIRT1 and NF-kB expression in SD model breast cancer that induced with DMBA. There is a positive correlation between SIRT1 and NF-kB expression in EPI-treated SD rats that were induced by DMBA

PENENTUAN AKTIVITAS ANTIBAKTERI EKSTRAK ISOLAT FUNGI ENDOFIT DARI DAUN GALING-GALING (Cayratia trifolia L) Ayyub Harly Nurung; Fitriana Fitriana; Herwin Herwin
As-Syifaa Jurnal Farmasi Vol 14, No 1 (2022): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jifa.v14i1.785

Utilization of wild plants around us needs to be done to expand the chances of wild plants being used as traditional medicine. These plants have the main content of the active compounds in the form of steroids, terpenoids, flavonoids, tannins and is reported to have antibacterial activity. This research aims to determine the concentration of endophytic fungi isolates IDGG 02, IDGG 03 and IDGG 06 from the leaves of galing-galing (Cayratia trifolia L). The first stage purification isolates of endophytic fungi in order to obtain single isolates. Then fermented using a shaker for 21 days at room temperature. Fermented extracted with ethyl acetate solvent using a liquid-liquid extraction methods to obtain a dry extract. Testing the minimum inhibitory concentration (MIC) with concentrations of 2048, 1024, 512, 256, 128, 64, 32, 16, 8 and 4 µg / mL and testing for antibacterial activity using agar diffusion methods against bacteria Staphylococcus aureus, Staphlococcus epidermidis, Escherichia coli and Vibrio cholerae.The results obtained MIC 2048 MIC ug / mL to extract isolates IDGG 02 and IDGG 06, while the extract obtained 03 isolates IDGG 1024 MIC ug / ml against all bacteria. Results of testing the antibacterial activity of endophytic fungi isolated extract IDGG 02, 03 IDGG produce diameter zone of inhibition at a concentration of 2048 mg / mL against Staphylococcus aureus, Staphlococcus epidermidis, Escherichia coli and Vibrio cholerae.

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