Claim Missing Document
Check
Articles

Found 6 Documents
Search

Pengaruh Senyawa Kimia Dalam Limbah Penyulingan Minyak Atsiri Terhadap Aktivitas E.coli Anugrahwati, Mai; Wicaksono, Wiyogo Prio; Nurlestari, Rizqy
Journal of Aquaculture Science Vol 2, No 1 (2017): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.255 KB) | DOI: 10.31093/joas.v2i1.17

Abstract

Effect of chemical compounds in the wastes of essential oil distillation including lime leaves, lemongrass leaves, ylang-ylang flower, and coriander seed on the activity of Escherichia coli has been performed. In this research, observation was done by using microbial fuel cell (using E.coli) with two compartments were connected by nafion membrane and zinc plats were used as electrodes which were connected by copper wire and multimeter. Energy production from the cell was shown from the generated current due to the E.coli’s oxidation activity to the substrates (distillation wastes). Results from the research exhibited that chemical compounds in the lime leaves positively affected to the bacterial activity with the generated current present up to 20 µA. It is also observed that mass variation of the substrates significantly affected the current generation. Chemical compounds in the three other wastes were found to be not as effective as lime leaves because from the analysis of their methanol extracts by GC-MS indicated that these contained antibacterial compounds such as octadecanoic acid and rosifoliol. Conclusion from the research was not all of wastes from essential oil distillation have a potency to be used as substrates in the microbial fuel cell as some of these chemicals had an antibacterial property which negatively affected the E.coli bacterial activity in the cell. Keywords : microbial fuel cell, Escherichia coli, waste of essential oil distillation.
Co-Solvent Free Electrochemical Synthesis of Biodiesel Using Graphite Electrode and Waste Concrete Heterogeneous Catalyst: Optimization of Biodiesel Yield Wiyogo Prio Wicaksono; Sekar Asmara Jati; Ika Yanti; Prastika Krisma Jiwanti
Bulletin of Chemical Reaction Engineering & Catalysis 2021: BCREC Volume 16 Issue 1 Year 2021 (March 2021)
Publisher : Department of Chemical Engineering - Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.9767/bcrec.16.1.10310.179-187

Abstract

This study optimized a co-solvent free electrochemical method for biodiesel synthesis using graphite electrode and waste concrete heterogeneous catalyst. Various parameters were evaluated, including: applied voltage (9.6, 14.4, 19.2 V), catalyst particle size uniformity (unfiltered and filtered with 150 mesh), and reaction time (15, 30, 120, 240 min). The results obtained 100% FAME content and 78.51% of biodiesel yield that were achieved at 14.4 V within 30 min using filtered catalyst and cooking oil feedstock. However, a slight decline was observed with the use of waste cooking oil. This optimized method offers a reliable and simple condition for mass biodiesel production. Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0). 
Effect of Chemical Compounds in Wastes from Essential Oil Distillation on the Activity of E.coli Mai Anugrahwati; Wiyogo Prio Wicaksono; Rizqy Nurlestari
Journal of Aquaculture Science Vol 2 No 1 (2017): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v2i1.17

Abstract

Effect of chemical compounds in the wastes of essential oil distillation including lime leaves, lemongrass leaves, ylang-ylang flower, and coriander seed on the activity of Escherichia coli has been performed. In this research, observation was done by using microbial fuel cell (using E.coli) with two compartments were connected by nafion membrane and zinc plats were used as electrodes which were connected by copper wire and multimeter. Energy production from the cell was shown from the generated current due to the E.coli's oxidation activity to the substrates (distillation wastes). Results from the research exhibited that chemical compounds in the lime leaves positively affected to the bacterial activity with the generated current present up to 20 µA. It is also observed that mass variation of the substrates significantly affected the current generation. Chemical compounds in the three other wastes were found to be not as effective as lime leaves because from the analysis of their methanol extracts by GC-MS indicated that these contained antibacterial compounds such as octadecanoic acid and rosifoliol. Conclusion from the research was not all of wastes from essential oil distillation have a potency to be used as substrates in the microbial fuel cell as some of these chemicals had an antibacterial property which negatively affected the E.coli bacterial activity in the cell. Keywords : microbial fuel cell, Escherichia coli, waste of essential oil distillation.
Virtual Laboratory in Chemistry: Recent Information and Communication Technologies (ICT) in Chemistry Practical Course Imam Sahroni; Wiyogo Prio Wicaksono; Maisari Utami; Lutfia Isna Ardhayanti
IJCER (International Journal of Chemistry Education Research) VOLUME 4, ISSUE 2, October 2020
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/ijcer.vol4.iss2.art4

Abstract

The covid-19 has impacted many sectors to change the way the run their activity, including the learning and teaching activity at a higher education. Many departments at the educational institution have been affected seriously due to the physical laboratory closing, especially for the activity at chemistry laboratory. The physical laboratory has been transformed to the virtual laboratory in order to accommodate the student to study the experimental course. By using the internet and communication technology (ICT) innovation in teaching and learning method, the student can still have the experiment and continue the lecture through online system. This effort may help the student to still play an active role while participating in the learning process.
Green synthesis, characterization, and electrochemical behavior of gold nanoparticles on boron-doped diamond electrode Wiyogo Prio Wicaksono; Aushofin Hamidah
Acta Chimica Asiana Vol. 1 No. 1 (2018)
Publisher : Chemistry Education Program, the University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (550.057 KB) | DOI: 10.29303/aca.v1i1.1

Abstract

Gold nanoparticles (AuNPs) have been successfully synthesized through a single-step bioreduction method using Piper betle leaf extract (PBLE) as a green reducing and stabilizing agent. Their formation was confirmed by UV-Visible spectroscopy, resulted in a characteristic Surface Plasmon Resonance (SPR) peak at 545 nm, whereas there is no peak observed in the extract. By using Particle Size Analyzer (PSA), it has been characterized that they have a ~100 nm diameter. For characterizing electrochemical behavior, the cyclic voltammetry technique was applied in a various range of electrolyte condition (HCl 0.1M, NaCl 0.1M, phosphate buffer 0.1M pH 7, and NaOH 0.1M). The best oxidation and reduction peak of the AuNPs were obtained in HCl at 0.93 V and 0.68 V, respectively. This green synthesized AuNPs could be applied as a label in immunochromatographic strip tests for various sensing applications.
Synthesis of Polyclonal Antibodies against Aflatoxin B1 Wicaksono, Wiyogo Prio; Permana, Deni Samsudin; Maryam, Romsyah; Einaga, Yasuaki
Makara Journal of Science
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Polyclonal antibodies of aflatoxin B1 were successfully produced from New Zealand White female rabbits after immunization by the hapten of aflatoxin B1-carboxymethyl hydroxylamine hemihydrochloride (AFB1-CMO) conjugated with bovine serum albumin (BSA) as the antigen. The hapten was synthesized using the carbodiimide method with CMO as a linker. Absorption peaks at 362, 264, and 218 nm were observed as a result of characterization with UV-Vis spectroscopy, while IR spectroscopy showed peaks at 3448 cm-1 and 1642 cm-1 attributable to the hydroxyl and nitrile groups, respectively. Furthermore, mass spectrometry showed fragmentation at the m/z of 386, 368.2, and 310, which confirms that the hapten of AFB1-CMO was successfully synthesized. The hapten was then conjugated with BSA to serve as an antigen of AFB1 when it was injected into the rabbits. The specificity of the antigen towards its antibody and the confirmation of hapten-BSA conjugation were characterized using the dot blot immunoassay, which showed a BSA concentration of 1.74 mg/mL. Two weeks after the primary immunization by its antigen, agar gel precipitation testing showed that the rabbit blood serum had positive results for polyclonal antibodiest against AFB1 with the highest concentration of antibodiest of 2.19 mg/mL.