Lilis Nuraida
1) South East Asian Food And Agricultural Science And Technology Center, Institut Pertanian Bogor, Bogor Dan 2) Departemen Ilmu Dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor

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EFEKTIVITAS PROGRAM PENINGKATAN MUTU DAN KEAMANAN PANGAN INDUSTRI RUMAH TANGGA PANGAN (IRTP) DI KABUPATEN CIANJUR Purba, Dreitsohn Franklyn; Nuraida, Lilis; Koswara, Sutrisno
JURNAL STANDARDISASI Vol 16, No 2 (2014): Vol 16, No 2 (2014)
Publisher : Badan Standardisasi Nasional

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Abstract

Peningkatan mutu dan keamanan pangan produksi IRTP bergantung pada efektifitas pembinaan dan pengawasan mutu dan keamanan pangan IRTP oleh Pemerintah Kabupaten Cianjur. Kajian ini bertujuan 1) mengidentifikasi regulasi yang dirujuk, program dan kegiatan, serta anggaran yang disediakan, 2) mengevaluasi penerapan CPPB IRT oleh IRTP, dan 3) memberikan rekomendasi. Data diperoleh dari berbagai dokumen Pemerintah Kabupaten Cianjur dan dari survei. Jumlah sampel 10% (71 IRTP), diambil dengan cara purposive sampling. Regulasi yang dirujuk cukup memadai tetapi belum lengkap, karena regulasi pokok untuk pembinaan IRTP tidak disosialisasikan seluruhnya. Pemerintah Kabupaten Cianjur belum mengembangkan regulasi mutu dan keamanan pangan IRTP. Program menghasilkan 82% IRTP memenuhi prasyarat dasar operasional, 11% belum memiliki SPP-IRT dan SPKP, dan 7% memiliki SPP-IRT tetapi tidak dapat menunjukkan SPKP-nya. Anggaran program masih kecil dan hanya difokuskan pada kegiatan penyuluhan keamanan pangan dan sertifikasi produksi pangan IRT; belum mencakup seluruh IRTP yang terdaftar pada Dinas Kesehatan Kabupaten Cianjur. Hampir 58,94% responden IRTP telah menerapkan beberapa parameter CPPB IRT dengan nilai baik, antara lain lingkungan produksi, peralatan produksi, fasilitas dan kegiatan hygiene dan sanitasi, kebijakan terhadap kesehatan karyawan, praktik sanitasi dan hygiene, dan penyimpanan produk. Hampir 38,78% dinilai masih kurang pada parameter suplai air dan pengolahan, pengendalian hama, praktik sanitasi, serta kemasan dan pelabelan. Berdasarkan analisis, pembinaan dan pengawasan mutu dan keamanan pangan IRTP di Kabupaten Cianjur dinyatakan berada pada tingkat efektivitas sedang. Pemerintah Daerah Kabupaten Cianjur hendaknya menggalakkan sosialisasi regulasi, menajamkan outcome program, memperbesar anggaran, dan meningkatkan mutu dan frekuensi penyuluhan, serta mengadvokasi/mendampingi IRTP.
Potency of Bioactive Compound of Rice Bran for Colon Cancer Prevention Safrida, Safrida; Budijanto, Slamet; Nuraida, Lilis; Priosoeryanto, Bambang Pontjo
KEMAS: Jurnal Kesehatan Masyarakat Vol 16, No 2 (2020)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v16i2.21133

Abstract

Colon cancer is the second leading cause of death in the world. Bioactive compounds in rice bran have a very active role as antiproliferation of colon cancer cells such as ferulic acid, p-coumaric acid, caffeic acid, gallic acid, protocatechuic acid, sinapic acid, tricin, luteolin, apigenin, myrecitin, rutin, isorhamnetin, γ-oryzanol, γ-tocopherol, δ-tocopherol, γ-tocotrienol, β-sitosterol, phytic acid, and hemicellulose. Mechanism of the bioactive compounds in cells varied, including modulation of a cell cycle, activation of immune cells, damage of a lipid layer and mitochondrial membrane, activation of caspase proteins, inhibition of protein cell tumor invasion, metastasis, and angiogenesis, and also acts as an antioxidant. Therefore, the existence of the scientific studies results of this review with the potential availability of adequate rice bran in Indonesia is very potential to be developed.
Preliminary Characterization of Protease Inhibitor from Bacteria-Associated with Sponge from Panggang Island, Seribu Islands TATI NURHAYATI; MAGGY THENAWIDJAJA SUHARTONO; LILIS NURAIDA; SRI BUDIARTI POERWANTO
HAYATI Journal of Biosciences Vol. 13 No. 2 (2006): June 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.733 KB) | DOI: 10.4308/hjb.13.2.58

Abstract

Pathogenic bacteria produced protease that involved in molecular mechanism of foodborne disease. Produced protease involved in molecular mechanisms of foodborne diseases. The purpose of this research was to screen, identify and characterize the potential microorganisms associated with sponge as producer of protease inhibitor. Among 96 isolates examined, four isolates i.e 10A6, 6A3, 9A51, and 1A12 yielded protease inhibitors which were potential to inhibit protease substrates (40-90%). One of the most potential protease inhibitor producer, the bacteria isolate 6A3, was identified as Chromohalobacter sp. Chromohalobacter sp.6A3 produced protease inhibitor with optimum temperature and pH 300 C and 5, respectively. The inhibitor activity was stable when incubated at 400 C for ten minutes or at 300 C for 8 hours. Key words: Bacteria, Chromohalobacter sp., protease inhibitor, screen, sponge
Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production . EFRIWATI; ANTONIUS SUWANTO; GAYUH RAHAYU; LILIS NURAIDA
HAYATI Journal of Biosciences Vol. 20 No. 2 (2013): June 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.422 KB) | DOI: 10.4308/hjb.20.2.57

Abstract

Yeasts and lactic acid bacteria (LAB) are commonly found in tempeh and has been studied separately. However, comprehensive study on population dynamics of yeasts and LAB during tempeh production, including the effect of the difference tempeh production methods has not been reported. This research was aimed in studying the effect of different methods of tempeh production applied in tempeh home industry on the dynamics of yeast and LAB communities. Population dynamics was expressed as both changes of colony number and its phylotype. Samples were obtained from five stages and from two different methods of tempeh production. Observations were carried out employing colony counting on selective media followed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). The study indicated that the population of yeasts and LAB during tempeh production were dynamic and different between these methods. Tempeh production methods affected the presence of yeasts and LAB population as indicated by difference in colony number, the number and diversity of phylotype, as well as number of specific phylotypes grew on plates.
Kajian Risiko Aflatoksin M1 dalam Produk Formula untuk Bayi dan Anak Usia 0-36 Bulan Yeni Restiani; Lilis Nuraida; Hanifah Nuryani Lioe
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 1 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (698.954 KB) | DOI: 10.18343/jipi.25.1.160

Abstract

Aflatoxin M1 (AFM1) is a carcinogenic compound found in milk-based products including infant and young children formula. The aim of this study was to determine the risk characterization of infants and young children to AFM1 through the consumption of formula products. For this purpose, the Estimated Daily Intake (EDI) for infants and young children was calculated by multiplying AFM1 concentration in 44 infant formulas, 53 advanced infant formulas, and 16 growing up formulas with consumption data. The concentration of AFM1 in these formula products were retrieved from food registration in Indonesian FDA from January 2015-June 2018. The average concentration of AFM1 in infant formula, advanced infant formulas, and growing up formula (lower bound-upper bound) were 0.0226-0.0335; 0.0418-0.0510; and 0.0038-0.0123 ng/g, respectively. The average EDI of AFM1 for infants and young children aged 0-6 months, 6-12 months, and 1-3 years based on individual consumption were 0.260-0.386, 0.282-0.343, 0.029-0.092 ng/kg body weight (BW)/day. The average EDI of AFM1 for infants and young children aged 0-6 months, 6-12 months, and 1-3 years based on the recommended consumption by food producer (lower bound-upper bound) were 0.403-0.598; 0.663-0.809; 0.031-0.098 ng/kgBW/day. The average hazard index (HI) values for infants aged 0-6 months and 6-12 months (lower bound-upper bound) were greater than 1 (one), i.e., 1.94-2.88 and 3.20-3.90, which indicates there is a health risk. However hazard index value for young children aged 1-3 years (lower bound-upper bound) were less than 1 (one), i.e., 0.15-0.47, which indicates a lower health risk. Keywords: advanced infant formula, aflatoxin M1, growing up formula, hazard index, infant formula
Karakteristik dan Evaluasi Prebiotik Tepung Biji Cempedak (Artocarpus champeden) Ummul Khayrah; Endang Prangdimurti; Lilis Nuraida
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 2 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.2.248

Abstract

This study aims to carry out chemical characterization and evaluate the prebiotic potential of cempedak (Arthocarpun champeden) seed flour. Cempedak seeds were processed by boiling and roasting, then dried and grinded. Boiled cempedak seed flour (BCF), roasted cempedak flour (RCF), and untreated cempedak seed flour (UCF) were analyzed proximately and measured the profiles of total starch and undigestable carbohydrates, which included resistant starch, total dietary fiber, insoluble dietary fiber, and soluble dietary fiber. Resistant starch content increased significantly (p<0.05) from 8% in UCF to 19% in BCF and RCF. Boiling and roasting treatments caused a decrease in SDF levels and an increase in IDF levels. The resistance of starch to hydrolysis by artificial gastric acid decreased after boiling. The value of prebiotic effect (PE) and prebiotic index (PI) showed a positive value of UCF on the growth of L. plantarum 1-S27202 (PE 0.27; PI 0.68) and the BCF on the growth of L. rhamnosus R23 (PE 0.18; PI 0.44). Keywords: Artocarpus champeden, chempedak seeds, resistant starch, dietary fiber, prebiotic
In Vivo Evaluation of Prebiotic and Synbiotic Properties of Processed Sweet Potato Products Lilis Nuraida; Hana .; Sri Rini Dwiari; Didah Nur Faridah
Jurnal Teknologi dan Industri Pangan Vol. 19 No. 2 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The aims of this research were to investigate the prebiotic and synbiotic properties of sweet potato products (combined with L. casei subsp Rhamnosus for probiotic) in increasing the number of Lactic Acid Bacteria (LAB) and suppressing the number of E. coli and the occurrence of Salmonella in vivo. Some previous study showed that sweet potato is a potent source of prebiotic. The sweet potato products evaluated were sweet potato flakes (SPF) and sweet potato ice cream mix. The in vivo assay used male rat strain Sprague-Dawley. Total microbes, LAB, E. coli and Salmonella in fecal were analyzed before, during and after feeding period. Feeding with SPF as prebiotic, L. casei subsp Rhamnosus as probiotic, and combination of both as synbiotic for ten days were able to increase the number of LAB (0.4-1.1 log CFU/g) and suppress the number of E. coli in rat feces (1.5-1.7 log CFU/g). All of the treatment did not affect the occurrence of Salmonella in rat feces. The treatment of sweet potato ice cream mix as prebiotics and the combination of sweet potato ice cream mix and L. casei subsp. Rhamnosus as synbiotic for ten days did not effect the number of LAB and E. coli in rat feces.Key words: sweet potato, prebiotic, oligosaccharide, probiotic, synbiotic
Antimicrobial Activity of Beluntas (Plucea indica L.) Leaves Extract and Stability of the Activityat Different Salt Concentrations and pHs Ardiansyah .; Lilis Nuraida; Nuri Andarwulan
Jurnal Teknologi dan Industri Pangan Vol. 14 No. 2 (2003): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Beluntas leaves are commonly used as traditional medical herb and fresh vegetables. The aim of the research was to examine the antimicrobial activity of beluntas leaves extracts and the stability of activity in salt and pH condition. Non defatted plar extract had phenol hidroquinon, tanin, alkoloid, and steroid as bioactive compound. The most sensitive bacteria was B. cereus and the most resistant was S. typhi. The MICs of polar extract against, S. typhi, S. aureus, E. coli, P. Fluorescens, B. subtillis, and B. cereus were 3.19, 2.94, 2.66, 2.64, 2.40, and 2.26% respectively. Antimicrobial activity was greater on protoplast of S. typhi sphaeroplast of S. aureus and Bacillus cereus than toward their whole cells, with the highest inhibitionobserved B. cereus protoplast. Addition of salt into the extract increased it's the antimicrobial activity. There was synergism between low pH with bioactive compound from non defatted polar extract. Key words : Beluntas, bioactive compound, MIC, protoplast, sphaeroplast, salt, pH.
PENGGUNAAN BERBAGAI JENIS BAHAN PELINDUNG UNTUK MEMPERTAHANKAN VIABILITAS BAKTERI ASAM LAKTAT YANG DI ISOLASI DARI AIR SUSU IBU PADA PROSES PENGERINGAN BEKU [Utilization of various cryogenic agents during freeze drying to Maintain the viability of Lactic Ni Nyoman Puspawati; Lilis Nuraida; Dede Robiatul Adawiyah
Jurnal Teknologi dan Industri Pangan Vol. 21 No. 1 (2010): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Lactic acid bacteria are the  most important bacteria having potential as probiotic. The objectives of the present study were to examine the growth of Lactic Acid Bacteria,  identify the Lactic Acid Bacteria capable of surviving and  evaluate the best cryogenic agents that  protect the viability of  Lactic Acid Bacteria during freeze drying. Four cryogenic agents, i.e. sucrose, lactose, skim milk and maltodextrin, were used in freeze drying of three species of Lactic Acid Bacteria, i.e. Pediococcus pentosaceus A16, Lactobacillus brevis A17 and Lactobacillus rhamnosus R21 isolated from breast milk.  Evaluation included viability before and after freeze drying, survival of freeze dried culture in 0.5 % bile salt and low pH for 5 hours. The result showed that three of cryogenics, i.e. sucrose, lactose and skim milk improved the viability of freeze dried of all lactobacilli, except maltodextrin that did not give protection to L. rhamnosus R21. Evaluation on the survival  of LAB in 0.5 % bile salt showed that cryogenic agents improved the survival rate of all Lactic Acid Bacteria during freeze drying. The cryogenic  also improved the survival rate of LAB at low pH, with the best protection given by skim milk on L. rhamnosus R21.
EVALUASI IN VITRO TERHADAP KEMAMPUAN ISOLAT BAKTERI ASAM LAKTAT ASAL AIR SUSU IBU UNTUK MENGASIMILASI KOLESTEROL DAN MENDEKONJUGASI GARAM EMPEDU [In Vitro Evaluation of Cholesterol Assimilation and Bile Salt Deconjugation by Lactic Acid Bacteria Isolated Lilis - Nuraida; Siti - Winarti; Hana - -; Endang - Prangdimurti
Jurnal Teknologi dan Industri Pangan Vol. 22 No. 1 (2011): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Hypercholesterolemia is a risk factor for cardiovascular disease, the leading cause of death in many countries. Several studies have shown that reduction of excessive levels of cholesterol in the blood decreases the risk of cardiovascular disease. It is therefore important to develop ways of reducing serum cholesterol. Based on in vitro and in vivo studies, some of lactic acid bacteria (LAB) having potential probiotic properties can reduce total cholesterol and low-density lipoprotein cholesterol levels. The aim of this study was to evaluate the ability of LAB isolated from breast milk in reducing cholesterol by assimilation and by bile salt deconjugation activity in vitro.Thirteen strains of LABs were evaluated for their acid and bile salt resistance and selected to test their ability to assimilate cholesterol and to deconjugate bile salt (natrium taurocholate) in vitro. Cholesterol assimilation activity was determined by measuring the difference between the remaining cholesterol in broth medium inoculated with LAB with cholesterol in control after incubation. Bile salt deconjugation activity was determined by measuring free cholic acid released in broth medium after incubation with LAB. The results shows that most of the isolates was susceptible to low pH and all isolates used were able to survive in the presence of 0.5% bile salt. The LAB were also able to assimilate cholesterol at varying levels ranging from 0.86-14.97 µg/ml, with the highest activity showed by Pediococcus pentosaceus 1-A38, Pediococcus pentosaceus 2-B2 and Pediococcus pentosaceus 2-A16. Taurocholate deconjugation assay showed that the isolates have weak bile salts deconjugation activity as indicated by free cholic acid released ranging from 0.06-0.25 µmol/ml, with the highest release in Pediococcus pentosaceus 1-A38 and Pediococcus pentosaceus 1-A22. The present study suggest that Pediococcus pentosaceus 1-A38 was potential for the development of probiotic products with specific benefit to reduce cholesterol through cholesterol assimilation and deconjugation of bile salt.
Co-Authors . EFRIWATI . Hana Afifah Zahra Agista Amor Tresna Karyawati, Amor Tresna Anja Meryandini Anja Meryandini Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R Antung Sima Firlieyanti Antung Sima Firlieyanti Apriliana W. Hartanti Ardiansyah . Bambang Pontjo Priosoeryanto Betty Jenie Sri Laksmi Betty Sri Laksmi Betty Sri Laksmi Jenie Betty Sri Laksmi Jenie Budi Nurtama Cece Sumantri Cynthia Rizka Riani Dahrul Syah Dede R. Adawiyah Dede Robiatul Adawiyah Denny Agustin Desty Gitapratiwi Desty Gitapratiwi Dewi, Fitriya Nur Annisa Dian Herawati Didah Nur Faridah Dodik Briawan Donna Fujie Rahaditha Utami Dreitsohn Franklyn Purba Eko Hari Purnomo Emmawati, Aswita Endang Prangdimurti Epi Taufik Fadlillah, Hendry Noer Faleh Setia Budi Farida, Eko Fenny Larasati Firat Meiyasa Gayuh Rahayu Hana - - Hana . Hana . Hanifah Nuryani Lioe Haqqifizta Ratihwulan Hendry Noer Fadlillah Irma Isnafia Arief Jamal Zamrudi Madaniyah, Siti Maggy T. Suhartono Maggy Thenawidjaja Suhartono Mochammad Sriduresta Soenarno Ni Nyoman Puspawati Nur Rita Mardiana Nurdin, Qamariyah Nuri Andarwulan Nurwulan Purnasari Pika Mustika Purwiyatno Hariyadi Puspo Edi Giriwono Qamariyah Nurdin Raini Panjaitan Ratih Dewanti -Hariyadi Ria Noviar Triana Rina Ningtyas Safrida Safrida Siti - Winarti Siti Madanijah Siti Madaniyah Slamet Budijanto Sri Budiarti Poerwanto Sri Rini Dwiari Suci Apsari Pebrianti Sutrisno Koswara TATI NURHAYATI Tita Rialita Tjahja Muhandri Ummul Khayrah Uswatun Hasanah Widya Aprilita Minamilail Winiati P Rahayu Winiati P. Rahayu Winiati Pudji Rahayu Yati Maryati Yati Maryati Yeni Kurniati Yeni Restiani Yuli Sukmawati YULIN LESTARI Zamrudi, Jamal Zulaikhah Zulaikhah Zulaikhah Zulaikhah Zulaikhah Zulaikhah, Zulaikhah