International Journal of Agricultural Sciences
Vol 1, No 1 (2015)

Specific RAPD frafments related with sex type in Salacca (Salacca edulis L.)

Ediwirman, Irfan Suliansyah, Gustian and Jamsari (Unknown)

Article Info

Publish Date
15 Aug 2017

Abstract

Salacca (Salacca edulis L.) is a tropical plant with a high economic value. The sexes of salacca can be grouped as dioecious, monoecious, dan hermaphrodite. Farmer's success in cultivation is especially determined by the male to female ratio. The present ratio of male and female plants is 1:4. Determining sex based on morphological characteristics is not effective and difficult for young plants (seedlings), also to lysozim marker and chromosome. Marker Assisted Selection is a strategy to determine sex using a marker. Random Amplifed Polymorfism DNA (RAPD) is an amplification technique that uses markers. Of 305 primers tested, 4 primers, OPO-17 produced 297 bp product, OPAP-20 produced 554 bp as the specific fragment for the female and UBC-454 produced 934 bp as the specific fragment for the female and UBC-454 produced 946 bp as male, primer UBC-78 with the size of fragment 562 bp as the specific fragment for the female.

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Journal Info
International Journal of Agricultural Sciences
Website

Abbrev

ijac

Publisher

Universitas Andalas

Subject

Agriculture, Biological Sciences & Forestry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Environmental Science Social Sciences

Description

International Journal of Agricultural Sciences is an open access journal published by the Graduate Program of Andalas University. IJASC publishes original research findings from throughout the world related to strategic and applied studies in all aspects of agricultural science, as well as reviews ...