International Journal of Biosciences and Biotechnology
Vol 1 No 2 (2013)

ZYMOGRAPHY OF EXTRACELLULAR PROTEASES IN Bacillus subtilis

Takeko Kodama (Unknown)
Keiji Endo (Unknown)
Katsutoshi Ara (Unknown)
Katsuya Ozaki (Unknown)
Junichi Sekiguchi (Unknown)



Article Info

Publish Date
30 Jul 2013

Abstract

In Bacillus subtilis, AprE, Bpr, Epr, Mpr, NprB, NprE, Vpr and WprA have been identifi ed as extracellularproteases. We determined protease activities from the culture supernatant of Bacillus subtilis using a 1Dzymogram gel containing gelatin. The highest protease activities were found at the late stationary phase ofa 75-h culture. To distinguish the proteases, the zymogram patt ern of the wild type was compared with theprotease-defi cient mutants (aprE, bpr, epr, mpr, nprB, nprE, vpr and wprA). Activities of AprE, Bpr, Mpr, andVpr were estimated by gelatin zymography. In addition, for the aprE mutant, the zymogram profi le of Bprwas very diff erent from that of the wild type. This suggested that AprE is involved in processing the Bprprotein.

Copyrights © 2013






Journal Info

Abbrev

jbb

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Environmental Science

Description

International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of ...