Vol 10 No 1 (2009)

Characterization of Edamame Indigenous Rhizobia as a Candidate of Biofertilizer

Arimurti, Sattya (Unknown)

Article Info

Publish Date
03 Jan 2009


Five isolates, named R1, R3, R4, R6 and R7, were successfully isolated from leguminous edamame nodules, and characterized as indigenous rhizobia bacteria. All isolates were grown in a YEMA medium containing antibiotics ampicillin, streptomycin, rifampicin, tetracycline, chloramphenicol or penicillin. Cultivation revealed that R3 can grow in a medium containing all antibiotics, but not for R1 when they grow in a medium containing rifampicin. R7 could not grow when the medium contain streptomycin and rifampicin. Furthermore, R4 and R6 only grow at medium containing tetracycline. it seemed that R1 and R3 are more resistant against some antibiotics comparing with others. When YEMA containing bromthymol blue 1% medium was used, R1 produced the yellowish acid and R3 produced blue alkali. R1 also utilized dulcitol and Lhistidin as carbon and nitrogen source. R3 utilize the carbon source from dulcitol but cannot utilize the nitrogen source from L-histidin. Base on these results above, it can be suggested that R1 and R3 identified as Rhizobium leguminosarum and Bradyrhizobium japonicum.

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Journal Info





Biochemistry, Genetics & Molecular Biology Chemistry Mathematics Physics


Jurnal ILMU DASAR (JID) adalah jurnal ilmiah yang melingkupi bidang matematika, fisika, kimia dan biologi. Naskah yang diusulkan untuk diterbitkan Jurnal Ilmu Dasar adalah naskah yang belum pernah diterbitkan dan atau tidak sedang dipertimbangkan penerbitannya di majalah lain. Jurnal Ilmu Dasar ...