Jurnal Ilmu Dasar
Vol 8 No 2 (2007)

Production and Characterization of Enzyme β-Endoxylanase from Bacteria of Termite-intestinal System

A. A. Istri Ratnawati (Unknown)
Wuryanti Handayani (Unknown)
Ni Nyoman Tri Puspaningsih (Unknown)

Article Info

Publish Date
05 Jul 2007

Abstract

A mesophile bacteria isolated from intestinal systems of local-soil termite, produced an extracellular β-endoxylanase upon growth on xylan. Optimum production of the enzyme was found in media containing oatspelt xylan at 37oC after sixth hours. The activities of its β-endoxylanase on oat-spelt xylan was investigated. It had an optimum pH and temperature, 5.0 and 40o C, respectively. However, pH stability occurred between 5.08.0. The enzyme was stable at 40o C for four hours and possessed a half life of four hours. β-endoxylanase had an apparent molecular mass of 45.000 to 66.200 Dalton as determined by SDS-PAGE. Analysis of zymogram using SDS-Xylan-PAGE indicated that enzymes could degrade oat-spelt xylan as substrates.

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Journal Info
Jurnal Ilmu Dasar
Website

Abbrev

JID

Publisher

Universitas Jember

Subject

Control & Systems Engineering Mathematics

Description

Jurnal ILMU DASAR (JID) is a national peer-reviewed and open access journal that publishes research papers encompasses all aspects of natural sciences including Mathematics, Physics, Chemistry and Biology. JID publishes 2 issues in 1 volume per year. First published, volume 1 issue 1, in January ...