Jurnal Natur Indonesia
Vol 13, No 2 (2011)

Isolasi Karakterisasi dan Penentuan Kadar Laktalbumin Susu Sapi Fries Holdstein dengan Metode Lowry

Copriady, Jimmi (Unknown)
Azmi, Johni (Unknown)
Maharani, Maharani (Unknown)

Article Info

Publish Date
21 Nov 2012

Abstract

Lactalbumin is a major protein found in milk which has great function to human body. It is a component of wheyprotein in milk. Lactalbumin was isolated from cow’s milk of Fries Holdstein breed by centrifugation at high speed9500 rpm and gel filtration chromatography using sephadex G-50 produced Lactalbumin dissolved in Tris buffersolvent. Characterization of Lactalbumin by measuring the UV absorbance spectrum at 240 – 320 nm comparedwith the spectrum of standard Lactalbumin showed maximum peak at the same wavelength, 280 nm. Lactalbumincontent in milk determined by Lowry method was about 0,66 mg/ml in milk or 9,2% of whey.

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Journal Info
Jurnal Natur Indonesia
Website

Abbrev

JN

Publisher

Universitas Riau

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

JURNAL NATUR INDONESIA terbit sejak tahun 1998, merupakan jurnal ilmu sains yang menyajikan artikel mengenai hasil penelitian, pemikiran dan pandangan dari peneliti dan pakar dalam bidang biosains (ilmu dasar), meliputi biologi, fisika, kimia dan matematika. Jurnal Natur Indonesia melibatkan mitra ...