Looking for alternative material from other resource to substitution of flour in feed of fish have done in early time. This research was conducted on June - August 2016. Aimed of ths study was to determine the amount of fermented flour from Morinda citrifolia L in the feed which affect the growth, feed efficiency, feed digestibility and protein retention in tambaqui (Colossoma macropomum). This research used a completely randomized design (CRD) with one factor, 5 treatments and 3 replications. The treatment with substitution of soybean flour (SF) with fermented Morinda citrifolia L leaf flour (MF) were Controls (100% SF: 0% MF), P1 (90% SF: 10% MF), P2 (85% SF : 15% MF), P3 (80% SF : 20% MF), P4 (75% SF : 25% MF), contain of protein in the feed about 30%. Results this study showed that the best result was treatment P3 (80: 20%)with a specific growth rate of 3.03% / day, feed efficiency rate of 30.6%, 78.86% of feed digestibility and protein retention 24.99%.
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