BERITA BIOLOGI
Vol 4, No 4 (1998)

KULTUR JARINGAN KUNIR PUTIH (KAEMPFERIA ROTUNDA L.)

Hoesen, Djadja Siti Hazar (Unknown)



Article Info

Publish Date
25 Feb 2016

Abstract

Shoot cultures of Kaempferia rotunda L.were established from rhizome segment.In the 1st experimen these explants were planted on Gamborg/B5 medium that supplemented with BA concentrations were (0, 0.5, 1 and 2) mg/l and/or kinetine were (0, 2 and 4) mg/l.In 2nd experiment these explants planted in Murashige and Skoog medium (MS).This medium supplemented with BA concentrations (0, 2 and 4) mg/l and/or NAA (0 and 1) mg/l.The result in the 1st experiment showed that the best proliferated shoots was from the culture that supplemented with BA 1 mg/l and kinetine 4 mg/l, while in the 2nd experiment the best proliferated shoots was from the culture that supplemented with BA 4 mg/l and NAA 1 mg/l.These shoots were then subcultured on MS liquid medium supplemented with BA (5 mg/l) and MS agar medium was supplemented with BA (2 mg/l) + 2iP (0.5 mg/l) + thidiazuron (0.01 mg/l) + NAA (0.5 mg/l).The cultures could induce the shoots number and produce the morphological plantlets for acclimatization,and acclimatization successed on soil and compost mixed medium in ratio 1:1.

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