Omni-Akuatika
Vol 15, No 2 (2019): Omni-Akuatika November

Study of Symbiont Bacteria of Acropora digitifera Coral From Ciletuh Bay, Sukabumi by Using Culture and Molecular Approach

Nurfitri Nurfitri (Study Programme of Marine Science, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran Jl. Raya Bandung-Sumedang KM 21 Jatinangor, Sumedang, West Java 45363 Indonesia)
Fiddy Semba Prasetiya (Universitas Padjadjaran Department of Marine Sciences Faculty of Fisheries and Marine Sciences)
Indah Riyantini (Department of Marine Science, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran Jl. Raya Bandung-Sumedang KM 21 Jatinangor, Sumedang, West Java 45363 Indonesia)
Yuniar Mulyani (Department of Fisheries, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran Jl. Raya Bandung-Sumedang KM 21 Jatinangor, Sumedang, West Java 45363 Indonesia)
Sulastri Arsad (Research Center of Molecular Biotechnology and Bioinformatics, Universitas Padjadjaran, Jl. Singaperbangsa No. 2, Bandung, West Java 40132 Indonesia)
Mochamad Untung Kurnia Agung (Department of Marine Science, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran Jl. Raya Bandung-Sumedang KM 21 Jatinangor, Sumedang, West Java 45363 Indonesia)



Article Info

Publish Date
26 Dec 2019

Abstract

Bacteria are one of the prokaryotic microorganisms that are symbiotic with coral reefs. These microorganisms help corals in secreting mucus layers which are used as a place to live for bacteria and control the presence of pathogenic bacteria in corals. The purpose of this study was to determine the diversity of bacterial communities associated with Acropora digitifera corals in Ciletuh bay, West Java, by culture approach. Sampling was carried out at a depth of 4 meters. Morphology-based identification and molecular approach based on sequence of the small ribosomal unit (16S) rRNA gene were used to determine the bacterial species in the samples. A total of six pure isolates were identified based on morphological observation and the molecular DNA characteristics from three of them were identified with 16S rRNA gene sequences. The identification using 16S rRNA gene showed that the isolate ACD.P4.PH7.P had a close relationship with the BF strain and zb strain of Bacillus flexus (acc number MH569560.1) with a similarity of 85.44 %. While ACD.P4.PH9.P isolate has a close relationship with Bacillus sp. c234 (acc number FJ950647.1) with a similarity of 98.50 %. Additionally, ACD.P4.PH9.K isolates closely related to Bacillus sp. of strain 6RM1 (acc number MK134607.1) with a similarity of 94.78 %. This study also revealed that both microscopic identifications by morphological traits and molecular approach using 16S rRNA gene can be used in bacterial diversity assessment.

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Journal Info

Abbrev

joa

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

Description

OmniAquatika is a scholarly refereed research journal which aims to promote the theory and practice, innovation, engineering and management as well as social-economic relevant in fisheries and marine sciences fields. The main aspects of research areas include, but are not exclusive to the current ...