Complication in Plasmodium falciparum malaria is associated with endothelial damage and overproductionof free radicalsÂ (oxidative stress) by activated neutrophils and endothelial cells. Red fruit (Pandanus conoideus) oil Â has a very highÂ antioxidant content, therefore it may neutralize free radicals and prevent endothelial damage. The aim of this research isÂ to prove that red fruit (Pandanus conoideus) oil reduce Reactive Oxygen Intermediate (ROI) production Â of endothelialÂ cells exposed to severe malaria patient serum and neutrophils from healthy donor. Endothelial cells from human umbilicalÂ veins were coincubated with serum from severe malaria patient and with neutrophils from healthy donor Â (positive controlÂ group). HUVECâ€™s normal was used as negative control, Â where as Â the experimental groups were given with red fruit oil inÂ different concentrations (2.8%, 5.7% dan 11.3% ). The Reactive Oxygen Intermediate (ROI) production of Â endothelialÂ cells was semiquantitatively measured by using NBT-reduction assay and the score of ROI was counted. The resultsÂ were statistically analyzed with ANNOVA (p<0.01). The rate of ROI production of endothelial cells was markedlyÂ increased after incubation with patient serum and neutrophils.
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