Majalah Biomorfologi (Biomorphology Journal)
Vol. 29 No. 2 (2019): Majalah Biomorfologi

EVALUASI JARINGAN DALAM STUDI IN VIVO PEMBERIAN EKSTRAK PROPOLIS PADA MODEL LUKA INFEKSI

Winona May Hendrata (Fakultas Kedokteran, Universitas Airlangga)
Willy Sandhika (Departemen Patologi Anatomi, Fakultas Kedokteran, Universitas Airlangga)
Agung Dwi Wahyu Widodo (Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Airlangga)



Article Info

Publish Date
15 Nov 2019

Abstract

Background: Infection in wound could lead to various consequences including scarring and occurrence of sepsis. With increasing prevalence of resistant strain bacteria, option for treating infected wound need to be constantly expanded. Propolis, a product of honey bee, is a potential treatment which has been studied to have various effects including antibacterial, anti-inflammatory, and antioxidant. Objective: The study aims to assess the effect of propolis toward wound healing, by evaluating wound macroscopic and microscopic appearance. Material and method: Propolis extract were made using maceration technique using ethanol. Thirty Rattus norvegicus male rats were divided into six groups: three groups for treatment and three groups for control. Incision wound were made in the back and was infected with Methicillin ResistantStaphylococcus aureus (MRSA). Propolis was administered orally on daily basis. Wound excision for observation were done on day3, 5, and 7. The tissue were processed using haematoxylin-eosin (HE) staining for microscopic observation. The distance of normal tissue surrounding the wound were measured using image analysis program. Result: Macroscopic observation showed fewer visible wound in the propolis extract treated group. Measurement by microscopic observation on day 3 also demonstrated more narrow wound width in propolis extract treated group (405.75  110.11 μ) compared to the control group (806.20 644.41μ). Conclusion: The result of this study suggests that propolis extract by oral administration promotes the healing of infected wound as shown within microscopic and microscopic observation.

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Journal Info

Abbrev

MBIO

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience Public Health

Description

Majalah Biomorfologi publishes articles in the form of original research report, excel-literature review, communication and special case report. The scope of this journal includes Medicine and Health sciences, focusing in basic and applied Anatomy and Histology (i.e., neuroscience, medical and ...