Unesa Journal of Chemistry
Volume 2, Number 1, 2013

UJI AKTIVITAS BAKTERI KITINOLITIK DARI TAMBAK UDANG DI LAMONGAN DAN SIDOARJO (ACTIVITY ASSAY OF CHITINOLYTIC BACTERIA FROM SHRIMP PONDS IN LAMONGAN AND SIDOARJO)

FAUZIAH, (Jurusan Kimia FMIPA Unesa)



Article Info

Publish Date
29 Jan 2013

Abstract

Abstrak. Bakteri kitinolitik telah berhasil diisolasi dari tambak udang di Lamongan dan Sidoarjo denganmenggunakan media yang mengandung 0.3% kitin koloidal. Diperoleh 54 isolat dari Lamongan yang diberi kode LA 1sampai LA 54 dan 78 isolat dari Sidoarjo yang diberi kode SDA 1 sampai SDA 78. 36 isolat dari Lamongan dan 67isolat dari Sidoarjo menunjukkan aktivitas kitinase. Uji aktivitas kitinase secara kualitatif ditentukan berdasarkan zonabening yang ditunjukkan disekitar koloni. Uji aktivitas kitinase secara kuantitatif ditentukan berdasarkan Nasetilglukosaminyang dilepaskan menggunakan metode Monreal and Reese. Aktivitas kitinase tertinggi ditunjukkanoleh isolat LA 21 dan LA 41 masing-masing sebesar 0.731 dan 0.615 U/mL.Kata Kunci: bakteri kitinolitik, kitin koloidal, zona bening.Abstract. Chitinolytic bacteria had been isolated from shrimp pond in Lamongan and Sidoarjo by using media withcontaining 0.3% chitin colloidal. Produced 54 isolates from Lamongan labelled LA 1 until LA 54 and 78 isolates fromSidoarjo labelled SDA 1 until SDA 78. 36 isolates from Lamongan and 67 isolates from Sidoarjo showed chitinaseactivity. Qualitatively assay of chitinase activity determined by clear zone around the colony. By using the method ofMonreal and Reese, quantitative assay of chitinase activity can be determined by the release of N-acetylglucosamine.The highest chitinase activity was shown by LA 21 dan LA 41 isolates are 0.731 and 0.615 U/mL respectively.Keywords: chitinolytic bacteria, colloidal chitin, clear zone.

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Journal Info

Abbrev

unesa-journal-of-chemistry

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

UNESA Journal of Chemistry is published online by the Department of Chemistry, Faculty of Mathematics and Natural Sciences, Surabaya State University, located at Jl Ketintang, Surabaya, East Java, Indonesia. 60231. Published 3 times a year (January, May and September) Aims and Scope: UNESA Journal ...