Unesa Journal of Chemistry
Volume 2, Number 2, 2013

PENGARUH PENAMBAHAN ION LOGAM K + TERHADAP AKTIVITAS ENZIM PAPAIN (THE ADDITION EFFECT OF THE METAL ION K+ ON THE PAPAIN ENZYME ACTIVITIES)

Soda, Fransiska Nay (Departmen of Chemistry, Surabaya State University Jl. Ketintang Surabaya (6023)telp. 031-8298761)
Agustini, Rudiana (Departmen of Chemistry, Surabaya State University Jl. Ketintang Surabaya (6023)telp. 031-8298761)



Article Info

Publish Date
24 May 2013

Abstract

Abstrak. Penelitian ini bertujuan untuk mendeskripsikan pengaruh penambahan ion logam K+terhadap aktivitas enzim papain. Penentuan pengaruh penambahan ion logam dilakukan denganmengukur aktivitas enzim tanpa penambahan ion logam K+ (0 mM) dan aktivitas enzim setelahditambah ion logam K+ dengan variasi konsentrasi 0,5 mM, 1 mM, 1,5 mM dan 2 mM. Hasilpenelitian menunjukkan bahwa ion logam K+ dapat meningkatkan aktivitas enzim papain dimanakonsentrasi optimum pada konsentrasi 1 mM dengan aktivitas sebesar 1,977 unit/mL. Padakonsentrasi 0, 5 mM, 1,5 dan 2 mM masing-masing dapat meningkatkan aktivitas enzim papainsebesar 1,131unit/mL, 1,552 unit/mL dan 1,043 unit/mL.Kata Kunci : Aktivator, Enzim papain, ion logam K+Abstract. This study aimed to describe the effect of the addition of metal ion K+ to papain enzymeactivity. Determination of the effect of the addition of metal ion is done by measuring the enzymeactivity without the addition of metal ion K+ (0 mM) and enzyme activity after addition of metalion with a variety of K+concentration of 0.5 mM, 1 mM, 1.5 mM and 2 mM. The results showedthat the metal ions K+ can increase the activity of the enzyme papain which the optimumconcentration at a concentration of 1 mM to the activity of 1,977 units /mL. At concentrations of0, 5 mM, 1.5 and 2 mM each of which can increase the activity of the enzyme papain 1,131 units/ mL, 1,552units /mL and 1,043 units /mL.Keywords : Activators, papain enzyme,, metal ion K+

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Journal Info

Abbrev

unesa-journal-of-chemistry

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

UNESA Journal of Chemistry is published online by the Department of Chemistry, Faculty of Mathematics and Natural Sciences, Surabaya State University, located at Jl Ketintang, Surabaya, East Java, Indonesia. 60231. Published 3 times a year (January, May and September) Aims and Scope: UNESA Journal ...