Unesa Journal of Chemistry
Vol 3, No 3 (2014): Volume 3, Number 3, 2014

AKTIVITAS BAKTERI AMILOLITIK YANG DIISOLASI DARI SUMBER AIRPANAS SINGGAHAN, TUBANACTIVITY OF AMILOLYTIC BACTERIA ISOLATED FROM HOT SPRING OFSINGGAHAN, TUBAN

PRATAMI PUTRI, UNIQUE (Unknown)



Article Info

Publish Date
01 Jan 1970

Abstract

Abstrak. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak kasar amilase dan mengetahui spesiesisolat aktivitas amilase tertinggiyang telah diisolasi dari Sumber Air Panas Singgahan, Tuban. Berdasarkanpenelitian sebelumnya, diperoleh 51 isolat yang telah positif menghasilkan amilase ditunjukkan denganclear zone. Lima puluh satu isolat tersebut diuji aktivitasnya menggunakan metode Miller (1959) denganreagen 3,5-Dinitrocalycilic acid (DNS). Diperoleh hasil isolat ST-21 memiliki aktivitas amilase tertinggiyaitu sebesar 1,4413 U/mL. Berdasarkan uji morfologi dan fisiologi isolat ST-21 menunjukkan spesiesBacillus amylolifaciens.Kata kunci:bakteri amilolitik termofilik, pati, aktivitas amilaseAbstract. This research aims tofound out amylase crude extract activity and found out species of isolates thathave highest activityisolated from hot spring Singgahan, Tuban. Building on last research, obtained 51isolates that positive produced amylase based on clear zone. One and fifty isolates tested their activity usedMiller metode (1959) with 3,5-Dinitrocalycilic acid (DNS) reagent. Obatained that isolate ST-21 that havethe highest amylaseactivityis 1,4413 U/mL. Based on morphology and phisiology ST-21 isolate shownspeciesBacillus amylolifaciens.Keywords:amylolytic thermophilic bacteria, starch, amylase activity

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Journal Info

Abbrev

unesa-journal-of-chemistry

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

UNESA Journal of Chemistry is published online by the Department of Chemistry, Faculty of Mathematics and Natural Sciences, Surabaya State University, located at Jl Ketintang, Surabaya, East Java, Indonesia. 60231. Published 3 times a year (January, May and September) Aims and Scope: UNESA Journal ...