Unesa Journal of Chemistry
Vol 8, No 1 (2019)

PENGARUH PENAMBAHAN NANOGOLD TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK GAMBIR (UNCARIA GAMBIR ROXB.) EFFECT OF NANOGOLD ADDITION TOWARD ANTIOXIDANT ACTIVITY OF EXTRACT GAMBIR (UNCARIA GAMBIR ROXB.)

NOVITA SARI, DIA (Unknown)
TAUFIKUROHMAH, TITIK (Unknown)



Article Info

Publish Date
13 May 2019

Abstract

Abstrak. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan nanogold pada berbagai konsentrasi terhadap aktivitas antioksidan ektrak gambir dan mengetahui konsentrasi nanogold yang terbaik untuk mendukung aktivitas antioksidan ekstrak gambir. Konsentrasi ekstrak gambir yang digunakan adalah 100 ppm dan nanogold konsentrasi 5, 10, 15, 20 dan 25 ppm. Pada penelitian ini dilakukan uji fitokimia senyawa fenolik terhadap gambir dan uji aktivitas antioksidan menggunakan metode DPPH dengan Spektrofotometer UV-Vis. Pada uji fitokimia menghasilkan bahwa ekstrak gambir positif mengandung senyawa fenolik ditunjukkan dengan terbentuknya warna coklat kehitaman. Pada penelitian ini aktivitas antioksidan ditentukan dengan persentase peredaman radikal bebas. Hasil menunjukkan bahwa penambahan nanogold pada ekstrak gambir memberi pengaruh dalam meningkatkan aktivitas antioksidan. Konsentrasi nanogold terbaik yang mendukung aktivitas antioksidan ekstrak gambir yaitu 25 ppm dan memiliki hasil peredaman radikal bebas terbaik yaitu sebesar 89,89 %. Hasil menunjukkan semakin besar konsentrasi nanogold, semakin besar pula persen peredamannya. Kata Kunci : Ekstrak Gambir, Nanogold, Aktivitas Antioksidan, DPPH, %Peredaman Abstract. The research aims to was to determine the effect of adding nanogold at various concentrations to the antioxidant activity of gambir extract and to know the best concentration for the addition of nanogold which supports gambir extract antioxidant activity. The gambir extrct concentration used was 100 ppm and nanogold concentration 5,10,15,20 and 25 ppm. In this research phytochemical test of phenolic compounds were performed and antioxidant activity test using DPPH method with using UV-Vis spectrophotometer. In phytochemical test resulted gambir extract that the positive extract contain phenolic compounds, shown by the formation of brown black. Antioxidant activity in this research was determined from decrease percentage of free radical. The results showed that the addition of nanogold to gambir extract gave influence in increasing the antioxidant activity. The best nanogold concentration that supports antioxidant gambir extract activity is 25 ppm and has the best result to support that is 89,89 %. This result showed that nanogold concentration, was higher then percentage reduction. Keywords : Gambir extract, Nanogold, Antioxidant activity, DPPH, % Reduction

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Journal Info

Abbrev

unesa-journal-of-chemistry

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

UNESA Journal of Chemistry is published online by the Department of Chemistry, Faculty of Mathematics and Natural Sciences, Surabaya State University, located at Jl Ketintang, Surabaya, East Java, Indonesia. 60231. Published 3 times a year (January, May and September) Aims and Scope: UNESA Journal ...