Unesa Journal of Chemistry
Vol 8, No 1 (2019)

PENGARUH EDTA TERHADAP AKTIVITAS LISOZIM SEBAGAI ANTIBAKTERI GRAM NEGATIF EFFECT OF EDTA ON LISOZIM ACTIVITY AS A NEGATIVE GRAM ANTIBACTERIAL

PERMATA SARI, DESI (Unknown)
HERDYASTUTI, NUNIEK (Unknown)



Article Info

Publish Date
13 May 2019

Abstract

Abstrak. Aktivitas antibakteri lisozim terbatas terhadap bakteri strain Gram positif. Untuk meningkatkan pemanfaatan lisozim yang dapat bekerja pada bakteri Gram negatif, dapat dilakukan dengan penambahan EDTA. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan larutan EDTA untuk mengubah aktivitas lisozim putih telur bebek menjadi antibakteri pada bakteri Gram negatif. Lisozim diperoleh dari ekstraksi menggunakan silika (SiO2). Penentuan profil protein putih telur bebek yang dilakukan dengan SDS-PAGE menunjukkan adanya pita protein disekitar 10-16 kDa yang diduga terdapat lisozim. Penambahan larutan EDTA dengan konsentrasi 1 mg/mL menunjukkan adanya pengaruh terhadap aktivitas antibakteri ekstrak lisozim. Kata Kunci : Aktivitas antibakteri, EDTA, Lisozim, Putih telur bebek. Abstract. Lysozyme antibacterial activity is limited to Gram positive bacterial strains. To increase lysozyme utilization that can work on Gram negative bacteria, it can be done by adding EDTA. This study aims to determine the effect of the addition of EDTA solution to change the lysozyme activity of duck egg white to antibacterial in Gram negative bacteria. Lysozyme is obtained from extraction using silica (SiO2). Determination of protein profiles of duck egg white carried out with SDS-PAGE showed the presence of protein bands around 10-16 kDa which were thought to be lysozyme. The addition of EDTA solution with a concentration of 1 mg / mL showed an effect on the antibacterial activity of lysozyme extract. Keywords : Antibacterial activity, EDTA, Lysozyme Duck egg white.

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Journal Info

Abbrev

unesa-journal-of-chemistry

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

UNESA Journal of Chemistry is published online by the Department of Chemistry, Faculty of Mathematics and Natural Sciences, Surabaya State University, located at Jl Ketintang, Surabaya, East Java, Indonesia. 60231. Published 3 times a year (January, May and September) Aims and Scope: UNESA Journal ...