Indonesian Journal of Dental Medicine
Vol. 1 No. 1 (2018): January - June

Toxicity Test on Taro Leaf Extract (Colocasia Esculenta L. Schoot) as Mouthwash to BHK-21 Fibroblast Cell Culture in Denture Users

Tiara Meilena (Faculty of Dental Medicine Universitas Airlangga)
Januar Cidie Fabiansyah (Faculty of Dental Medicine Universitas Airlangga)
Eha Djulaeha (Faculty of Dental Medicine Universitas Airlangga)
Hanoem Eka Hidayati (Faculty of Dental Medicine Universitas Airlangga)



Article Info

Publish Date
31 Mar 2018

Abstract

Background: One of requirements for a material to be used in dentistry is that the material must be non toxic and non irritant, as well as have biocompatibility properties, not triggering a detrimental effect on the biological environment, both locally and systemically. The first-line tests that must be conducted is in vitro tests, one of which is MTT Assay. Samples used in this research were fibroblast cells (cultured BHK-21 cells) since fibroblasts are the most important and largest components in pulp, periodontal ligament, and gingiva. Purpose: This research aimed to determine toxicity effect of taro leaf extracts (Colocasia esculenta L. Schoot) on BHK-21 fibroblast cells. Methods: This research is an experimental laboratory research using Post Test Only Control Group design.  The research treatment are the administration of Taro leaf extract (Colocasia esculenta L. Schoot)  with 100%, 80%, 60%, and 40% concentration on BHK-21 fibroblast cell. Results: The fibroblast cell life percentage in 100%, 80%, 60%, and 40% concentration respectively are 106,3%, 50,9%, 36,2%, and 30,1%. The toxicity results are obtained using MTT assay technique after 24 hours. The optical density absorbency values are read by ELISA reader and represent life cell viability. Conclusion: Taro leaf extract (Colocasia esculenta L. Schoot) at a concentration above 80% is considered as non-toxic material to BHK-21 fibroblast cells

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Journal Info

Abbrev

IJDM

Publisher

Subject

Dentistry

Description

Indonesian Journal of Dental Medicine accepts original manuscripts in the many fields of dentistry, including research reports and literature reviews. The spread of fields include: Oral epidemiology, Oral health services research, Preventive dentistry, Oral health education and promotion, Clinical ...