Jurnal AgroBiogen
Vol 7, No 1 (2011): Jurnal AgroBiogen

Delivering of Over-Expression Construct OsWRKY76 Candidate Gene in Rice cv. Nipponbare through Agrobacterium tumefaciens

Apriana, Aniversari ( Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian)
Sisharmini, Atmitri ( Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian)
Enggarini, Wening ( Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian)
Sudarsono, Sudarsono ( Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor)
Khumaida, Nurul ( Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor)
Trijatmiko, Kurniawan R. ( Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian)



Article Info

Publish Date
30 Apr 2013

Abstract

Plant genetic improvement can be done through classical breeding or genetic engineering. WRKY is a transcription factor involved in regulating plant defense responses. OsWRKY76 gene is located in a narrow segment of chromosome 9 which is identified previously to be related to wide spectrum resistance in rice. A sequence of OsWRKY76 (+1.200 bp) has available in the gene bank and it makes possible to isolate, clone, and construct the gene into over-expression vector. The aim of this research was to assemble an over-expression construct of OsWRKY76 candidate gene and introduce it into rice through Agrobacterium-mediated transformation. A construct of pCAMBIA-1301::35S::OsWRKY76 has been successfully assembled and transformed into embryogenic calli of rice cv. Nipponbare using A. tumefaciens strain Agl-1 and EHA 105. A number of 126 independent lines has been produced, in which Agl-1 showed 3.8 times more efficient than EHA 105. PCR analysis of randomly selected 25 independent lines showed that all of them positively contained hptII gene, a selectable marker used in the over-expression construct of the OsWRKY76 candidate gene. Based on the result, it could be concluded that the over-expression construct of OsWRKY76 candidate gene have been successfully introduced into the tissue of Nipponbare.

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Journal Info

Abbrev

JAB

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya ...