Berkala Ilmiah Mahasiswa Farmasi
Vol 6 No 1 (2019): Berkala Ilmiah Mahasiswa Farmasi Indonesia (BIMFI)

KIT DIAGNOSTIK DIFTERI BERBASIS HIBRIDISASI DNA

billgerd Tjengal (Institut Teknologi Bandung)
Almira Alifia (Institut Teknologi Bandung)
Novia Syari Intan (Institut Teknologi Bandung)



Article Info

Publish Date
30 Jun 2019

Abstract

Introduction: Diphtheria is an upper respiratory tract infection caused by Corynebacterium diphtheria bacterium. The recent diphtheria outbreak caused a large number of deaths in rapid period. This is due to the absence of a fast and precise method of diphtheria diagnosis. Current diagnosis practices aredone by cultivating bacteria which takes a relatively long time, which is 3 to 5 days, while the patients may already develop severe infection by the time the results are accessible. Therefore, a sensitive, easy and fast diphtheria diagnostic method is needed so that patients suspected of being infected can betreated quicker.Methods: This advertorial is written based on literature study method.Result: The diphtheria diagnostic kit was designed based on the DNA hybridization method by using DNA microarray. There are probes on the microarray plate in the form of a single stranded DNA fragment that is complement to the unique area of gene that encodes diphtheria toxin. The test sample wasobtained from the preparation of the patient's throat smear and labeled with a fluorescence marker before dropped onto the plate. Thus, if there is diphtheria DNA in the patient's sample, hybridization will occur between diphtheria DNA and the complementary DNA probe. The occurrence of hybridization will be characterized by fluorescence as an indicator of whether patients have diphtheria or not. The application of this diagnostic kit can shorten the diagnosis of C. diphtheria to less than 24 hours .Conclusion: Diphtheria diagnostic kit based on DNA hybridization can be made and applied.

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Journal Info

Abbrev

bimfi

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology

Description

BIMFI is a peer-reviewed and open access journal that prioritize the publication manuscript of pharmaceutical and health cluster students. This journal publishes editorial, research article, reviews, evidence-based case report, and also interesting case reports/case study. BIMFI receives manuscripts ...