Journal of Tropical Horticulture
Vol 3, No 2 (2020): October 2020

The Success Rate of Two Explant Types of Stevia (Stevia rebaudiana Bertoni) in Various Sterilant Formulas

Riedha Kariena (Agroecotechnology Department, Faculty of Agriculture, Universitas Lambung Mangkurat, Jl. Jend. A. Yani KM 36, Banjar Baru, Kalimantan Selatan, 70714,)
Nofia Hardarani (Agroecotechnology Department, Faculty of Agriculture, Universitas Lambung Mangkurat, Jl. Jend. A. Yani KM 36, Banjar Baru, Kalimantan Selatan, 70714,)
Hilda Susanti (Agronomy Department, Faculty of Agriculture, Universitas Lambung Mangkurat Jl. Jend. A. Yani KM 36, Banjar Baru, Kalimantan Selatan, 70714,)



Article Info

Publish Date
23 Nov 2020

Abstract

This study aims to determine the effect of interaction between several sterilants and types of explants and determine the best interaction with stevia culture's success rate. This study was designed using a Factorial Complete Randomized Design with two factors and three replications. The first factor is sterilant formulas i.e: fungicide 3% + alcohol 70% + Bayclin 5%; fungicide 3% + bactericide 6% + 70% alcohol + Bayclin 5%; sublimate 0.1% + 70% alcohol + Bayclin 5%; and fungicide 3% + bactericide 6% + sublimate 0.1% + alcohol 70% + Bayclin 5%. The second factor is explant types, i.e., stevia nodes and leaves. The variables observed are the percentage of contamination (%), percentage of alive explants (%), and browning percentage.  The interaction between sterilants and explant types only had a significant effect on the percentage of alive explants. The best interactions of sterilant formulas and explant types on the percentage of alive explants are 0.1% sublimate + 70% alcohol + 5% Bayclin and leaf explants.

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Journal Info

Abbrev

jthort

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

Journal of Tropical Horticulture (JTHort) ISSN 2622-8432 (online) is a peer-reviewed open-access international journal contains primary articles of research and review in horticulture such as fruits, vegetable, and ornamental plants also tropical biopharmaca plants. This journal is under the ...