Bioma : Jurnal Biologi dan Pembelajaran Biologi
Vol 6, No 1 (2021): BIOMA:JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI

AKTIVITAS ENZIM HIDROLASE PADA PENAPISAN ISOLAT ACTINOMYCETES TOLERAN SALINITAS

Sumardi, Sumardi (Unknown)
Qatrunada, Vanya (Unknown)
Farisi, Salman (Unknown)
Arifiyanto, Achmad (Unknown)
Ekowati, Christina Nugroho (Unknown)
Farisi, Salman (Unknown)



Article Info

Publish Date
31 Mar 2021

Abstract

Actinomycetesis one of the microorganisms which have the ability of enzymes hydrolases such as cellulose, amylase, protease, and manannase. Manufacturer of enzymes of the hydrolase can be used as a candidate probiotic. A candidate probiotic should have a good resistance in different environmental conditions including pH and salinity.  This research is conducted to filter the candidate’s probiotics from bacteria Actinomycetes that tolerance salinity and has the activity of the enzyme hydrolase. Testing the activity of the enzyme was preformed qualitatively on the variation of pH 4, 7, and 9.8.  Theability of Atinomycetesproduce enzymes hydrolases is indicated by the colony on media containing the substrate.From the test results, cellulase enzyme activity with the largest enzymatic index value is 3.5 AF2 isolates pH 9.8. Amylase enzyme activity with the largest enzymatic index value is 3.66 RH1 isolates pH 7. Protease enzyme activity with the largest enzyme index value is 0.66, AF2 isolates at pH 7. Mananase enzyme activity with the largest enzymatic index value is 0.85, namely AF2 isolates pH 4. In testing the effect of salinity test isolates were able to grow at variations in the concentration of NaCl 0, 3, and 6%.

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Journal Info

Abbrev

BIOMA

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

BIOMA : JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI adalah pupblikasi yang memuat artikel Pendidikan Biologi, Penelitian Murni Biologi, sumber, bahan, atau media pembelajaran Biologi / Sains (bahan pembelajaran Biologi di semua tingkat pendidikan dan aplikasi dalam masyarakat), Kurikulum Pendidikan ...