Bioscientia Medicina : Journal of Biomedicine and Translational Research
Vol. 5 No. 4 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research

Western Blotting (WB) Technique Guideline for Separation and Isolation of Protein

Rachmat Hidayat (Department of Biology, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia)
Patricia Wulandari (Cattleya Mental Health Center, Palembang, Indonesia)

Article Info

Publish Date
29 Jan 2021

Abstract

Western blotting is an important technique used in cell and molecular biology. Using the western blot, researchers can identify specific proteins from the complex mixture of proteins extracted from cells. This technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target proteins using appropriate primary and secondary antibodies to visualize. This paper will attempt to explain the techniques and theory behind western blot, and offer several ways to solve the problem

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Journal Info
Bioscientia Medicina : Journal of Biomedicine and Translational Research
Website

Abbrev

bsm

Publisher

Universitas Sriwijaya

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

Description

BioScientia Medicina is an open access international scholarly journal in the field of biomedicine and translational research aimed to publish a high-quality scientific paper including original research papers, reviews, short communication, and technical notes. This journal welcomes the submission ...