JURNAL PERIKANAN TROPIS
Vol 7, No 2 (2020)

AKTIVITAS ENZIM KATEPSIN PADA IKAN LELE (Clarias sp.) PADA PERLAKUAN SUHU DAN SUBSTRAT YANG BERBEDA

Anhar Rozi (Universitas Teuku Umar)
Ikhsanul Khairi (Universitas Teuku Umar)
Reni Tri Cahyani (Fakultas Perikanan dan Ilmu Kelautan, Universitas Borneo Tarakan, Kalimantan Utara)
Stephani Bija (Fakultas Perikanan dan Ilmu Kelautan, Universitas Borneo Tarakan, Kalimantan Utara)
Nurhikma Nurhikma (Fakultas Perikanan dan Ilmu Kelautan, Universitas Borneo Tarakan, Kalimantan Utara)
Nuring Wulansari (Pangkalan PSDKP Jakarta, Ditjen PSDKP)
Deden Yusman Maulid (Politeknik Kelautan dan Perikanan Pangandaran)
Siluh Putu Sri Dia Utari (Politeknik Kelautan dan Perikanan Jembrana)
Diah Anggraini Wulandari (Pusat Penelitian Bioteknologi, LIPI, Cibinong,Bogor)
Tati Nurhayati (Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor)



Article Info

Publish Date
05 Dec 2020

Abstract

Cathepsin was proteolytic enzyme which is found animal tissues included fish. Cathepsin found in fish muscle tissue, cathepsin and other hydrolyzing enzymes were place in subcellular organelles and divided into two places,  that was in muscle fibers and extracellular matrix. The aimed of this study was to the characterization of cathepsin enzyme especially catfish. So that in enviromental condition that can increased the activity of cathepsin enzyme an approtiate handling process can be carried out. The method of analyze enzyme activity, while the measurement of protein concentration refered to Bradford (1976). The treatments in this study was determination temperature (30oC, 40oC, 50oC, dan 60oC) and substrate (1.5% 2%, 2.5%). The result showed of enzyme activity with detrmination temperature very influential and fluctuating, the optimum temperature was 30oC where the activity reaches 0.38 U/ml. The best activity treatment of substrate was 1.5% where the activity reaches 0.42 U/ml. BSA Standard curve with regression equation y = 0.95x + 0.109.Keywords: Cathepsin, enzyme, substrate, temperature

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Journal Info

Abbrev

jptropis

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

Description

Jurnal Perikanan Tropis merupakan salah satu jurnal yang dikelola dan diterbitkan oleh Fakultas Perikanan dan Ilmu Kelautan, Universitas Teuku Umar. Jurnal Perikanan Tropis terbit pertama kali pada tahun 2015 dengan jumlah terbitan dua kali dalam setahun, yaitu pada bulan April dan ...