Jurnal Pengolahan Hasil Perikanan Indonesia
Vol 23 No 3 (2020): Jurnal Pengolahan Hasil Perikanan Indonesia 23(3)

Perbandingan Metode Isolasi DNA pada Produk Perikanan Segar dan Olahan: Comparison of DNA Isolation Methods for Fresh and Processed Seafood

Aninditya Artina Setiaputri (Unknown)
Giri Rohmad Barokah (Departemen Teknologi Hasil Perairan, FPIK IPB University)
Muh. Alsere Bardian Sahaba (Departemen Teknologi Hasil Perairan, FPIK IPB University)
Rahma Dini Arbajayanti (Departemen Teknologi Hasil Perairan, FPIK IPB University)
Nadia Fabella (Departemen Teknologi Hasil Perairan, FPIK IPB University)
Rizsa Mustika Pertiwi (Departemen Teknologi Hasil Perairan, FPIK IPB University)
Mala Nurilmala (Departemen Teknologi Hasil Perairan, FPIK IPB University)
Roni Nugraha (Departemen Teknologi Hasil Perairan, FPIK IPB University)
Asadatun Abdullah (Departemen Teknologi Hasil Perairan, FPIK IPB University)



Article Info

Publish Date
22 Dec 2020

Abstract

Seafood product is an important commodity in the global trade market, however, seafood fraud has been increasingly reported. Therefore, a method to specifically identify species of seafood products is needed. DNA methods gain many interests for detection of seafood fraud due to its robustness. This study was aimed to determine the effectiveness of several methods for isolation of DNA from fresh and processed seafood. The DNA isolation methods used in this study were the CTAB DNAzol, TianGen, Qiagen, and Fasmac.. DNA concentration and purity were determinedusing nanodrop spectrofotometry and electrophoresis. DNA isolation showed that the DNA concentration of fresh seafood products was higher than the processed seafood products. DNA isolation using CTAB method have higher levels of DNA purity (1.60-2.00) than using commercial kits in all samples. The commercial kits offer a simple procedure and minimize the possibility of environmental contaminants.

Copyrights © 2020






Journal Info

Abbrev

jphpi

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

JPHPI publishes manuscripts in the field of marine post-harvest, aquatic biotechnology, aquatic biochemistry, aquatic product diversification, and characteristic of aquatic raw materials. In addition, JPHPI also publishes research about aquatic product quality, standardization, and other researches ...