Natural Sciences Engineering and Technology Journal
Vol. 1 No. 2 (2021): Natural Sciences Engineering and Technology Journal

Enzyme-Linked Immunosorbent Assay (ELISA): A Narrative Literature Review

Maya Chandra Dita (Department of Obstetrics and Gynecology, Dr. Mohammad Hoesin General Hospital, Palembang, Indonesia)

Article Info

Publish Date
19 Aug 2021

Abstract

Enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) are both widely used as diagnostic tools in medicine and as quality control measures in many industries. This literature review was aimed to describe enzyme linked immunosorbent assay in practical. ELISA has been the system of choice when testing soluble antigens and antibodies. EIA/ELISA uses the basic immunological concept of antigen binding to specific antibodies, which allows the detection of small amounts of antigens such as proteins, peptides, hormones or antibodies in fluid samples. In all protocols, solid-phase reagents are incubated with secondary or tertiary reactants covalently coupled with the enzyme. The unbound conjugate is washed and a chromogenic or fluorogenic substrate is added. In conclusion, EIA / ELISA is a powerful method not only for general biomedial research but also as a diagnostic tool. This procedure allows the detection of all types of biological molecules at very low concentrations and quantities. Despite their limitations, EIA/ELISA remains an important tool in both clinical and basic research, and in clinical diagnosis.

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Journal Info
Natural Sciences Engineering and Technology Journal
Website

Abbrev

nasetjournal

Publisher

HM Publisher

Subject

Aerospace Engineering Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Electrical & Electronics Engineering Energy Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Social Sciences Transportation

Description

Natural Sciences Engineering and Technology Journal (NASET Journal) concern with publishing the original research articles, review articles from contributors, and the current issues related to engineering, technology, and natural sciences. The main objective of NASET Journal is to provide a platform ...