Journal of Aquatropica Asia
Vol 6 No 1 (2021): Journal of Aquatropica Asia

SKRINING BAKTERI TERMOFILIK POTENSIAL AMILOLITIK DARI SUMBER AIR PANAS WAY BELERANG KALIANDA LAMPUNG SELATAN

Sefi Desfeni Mawati (Unknown)
Esti Harpen (Unknown)
Hilma Putri Fidyandini (Unknown)

Article Info

Publish Date
06 Jul 2021

Abstract

Thermophilic bacteria that produced amylase and protease have been isolated from Way Belerang hot spring, Kalianda, South Lampung. This research aims to screen and identify thermophilic bacteria that have the potential to produce thermostable amylase and protease enzymes.The research procedures included sampling, isolation of enzyme-producing thermophilic bacteria, a series of phenotypic and biochemical tests, and molecular identification by 16s rRNA. This study used 2 treatments, namely incubation temperature 37 and 50 ºC with 3 repetitions. The results showed that the optimum temperature for growth of thermophilic bacterial isolates and thermophilic bacterial isolates producing amylase enzymes was 50ºC. The bacteria isolate that had the best amylolytic enzyme activity was Isolate A.WB.50.1 with a diameter of the inhibitory zone was 15.44 mm. Isolate A.WB.50.1 has been identified by the species Pseudomonas stutzeri.

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Journal Info
Journal of Aquatropica Asia
Website

Abbrev

aquatropica

Publisher

Universitas Bangka Belitung

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Journal of Aquatropica Asia (JoAA) is an open access scientific periodical managed by the Department of Aquaculture, Bangka Belitung University. JoAA Journal involvement is carried out 2 (two) times a year, namely in July and December in the form of the main manuscript is an article that contains ...