journal of Basic Medical Veterinary
Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021

Effect of Vaccination Outer Membrane Protein 52 kDa on Changes in Erythrocyte Index of Tilapia (Oreochromis niloticus) Infected by Aeromonas hydrophila

Nanda Rino Nurrahmad (Mahasiswa, Fakultas Kedokteran Hewan, Universitas Airlangga)
M. Gandul Atik Yuliani (Departemen Ilmu Kedokteran Dasar Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga)
Rahaju Ernawati (Departemen Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Airlangga)
Sri Chusniati (Departemen Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Airlangga)
Eduardus Bimo Aksono Herupradoto (Departemen Ilmu Kedokteran Dasar Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga)
Retno Sri Wahyuni (Departemen Ilmu Kedokteran Dasar Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga)

Article Info

Publish Date
26 Jul 2021

Abstract

The purpose of this study was to determine the effect after being vaccinated by OMP 52 kDa Aeromonas hydrophila for 1 week and then infected with Aeromonas hydrophila 10 CFU/mL for 4 days on changes in erythrocyte index. Tilapia (Oreochromis niloticus) used in this study was 10-12 cm long. There were 20 tilapia (Oreochromis niloticus) which were divided into 4 groups, consisting of 2 control groups and 2 treatment groups which were given various types of vaccine formulations. Group P0 (-) (unvaccinated and infected), Group P0 (+) (unvaccinated and infected), group P1 (vaccinated with the whole cell protein "HydroVac®" and infected), and P2 (vaccinated with Outer Membrane Protein 52 kDa and infected) by intramuscular injection. Post-treatment blood samples were collected on day 5 post-infection, collected through a caudal punctie and then analyzed using a hematology analyzer. Post-treatment outcomes led to statistically significant changes (p < 0.05). Therefore, the vaccine caused a significant change in the erythrocyte index.

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Journal Info
journal of Basic Medical Veterinary
Website

Abbrev

JBMV

Publisher

Universitas Airlangga

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Description

This journal published original articles, review articles, and case studies in Indonesian or English, in the scope of JBMV has a broad coverage of relevant topics across veterinary basic medical sciences which includes: preclinical and paraclinical disciplines like Anatomy, Physiology, Biochemistry, ...