Berkala Ilmiah Kimia Farmasi
Vol. 7 No. 1 (2020): JUNI

Validasi Metode Spektrofotometri UV Untuk Penetapan Kadar Kolkisin Dalam Infus Kembang Sungsang

Muhammad Muslich (Departemen Kimia Farmasi Fakultas Farmasi Universitas Airlangga)
Isnaeni Isnaeni (Departemen Kimia Farmasi Fakultas Farmasi Universitas Airlangga)
Sudjarwo Sudjarwo (Departemen Kimia Farmasi Fakultas Farmasi Universitas Airlangga)



Article Info

Publish Date
09 Aug 2021

Abstract

Validation of colchicine analysis methods in herbal infusions has been carried out to facilitate quality control of raw materials or simplicia. The validation parameters of the method set include selectivity, linearity, precision, and accuracy, which are included in category I. In this study, the raw material was prepared from the leaves of Gloriosa superba Linn. infusion made. The results of the selectivity test with colchicine as a standard provide uptake at a chosen wavelength of 340.2 nm. Linearity test results show the equation y = 0.0303x + 0.0131 with r = 0.9998> r Table for n = 6, ie 0.917 for p <0.01 and 0.811 for p = <0.05, the value of Vxo is 1 15%. In the precision test, the coefficient of variation is 0.54% and the accuracy test gives an average recovery of 92.81% ± 4.16%. Determination of colchicine in the infusion preparation was carried out as many as three replications. The results showed that the content of colchicine in raw materials was 0.3152% ± 1.99%, while the colchicine content in herbal infusion preparations was 0.483 ± 4.47%.

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Journal Info

Abbrev

BIKFAR

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Medicine & Pharmacology

Description

to facilitate interaction, discussion, and updating of research ideas in the fields of pharmacy, Pharmaceutical Chemistry, Medicinal Chemistry, Drug Development, Organic synthesis, Analytical chemistry, Pharmaceutical Technology, Pharmaceutical Microbiology and Biotechnology, Pharmacology and ...